Bypassing the Pentose Phosphate Pathway: Towards Modular Utilization of Xylose

Chomvong, Kulika; Bauer, Stefan; Benjamin, Daniel I.; Li, Xin; Nomura, Daniel K.; Cate, Jamie H.  D.

doi:10.1371/journal.pone.0158111

Bypassing the Pentose Phosphate Pathway: Towards Modular Utilization of Xylose

Journal Article · Thu Jun 23 00:00:00 EDT 2016 · PLoS ONE

DOI:https://doi.org/10.1371/journal.pone.0158111· OSTI ID:1627794

Chomvong, Kulika ^[1]; Bauer, Stefan ^[2]; Benjamin, Daniel I. ^[3]; Li, Xin ^[4]; Nomura, Daniel K. ^[3]; Cate, Jamie H. D. ^[5]

Univ. of California, Berkeley, CA (United States). Dept. of Plant and Microbial Biology; DOE/OSTI
Energy Biosciences Inst., Berkeley, CA (United States)
Univ. of California, Berkeley, CA (United States). Dept. of Nutritional Sciences and Toxicology. Program in Metabolic Biology
Univ. of California, Berkeley, CA (United States). Dept. of Molecular and Cell Biology
Univ. of California, Berkeley, CA (United States). Dept. of Molecular and Cell Biology; Univ. of California, Berkeley, CA (United States). Dept. of Chemistry; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Physical Biosciences Division

The efficient use of hemicellulose in the plant cell wall is critical for the economic conversion of plant biomass to renewable fuels and chemicals. Previously, the yeast Saccharomyces cerevisiae has been engineered to convert the hemicellulose-derived pentose sugars xylose and arabinose to D-xylulose-5-phosphate for conversion via the pentose phosphate pathway (PPP). However, efficient pentose utilization requires PPP optimization and may interfere with its roles in NADPH and pentose production. Here, we developed an alternative xylose utilization pathway that largely bypasses the PPP. In the new pathway, D-xylulose is converted to D-xylulose-1-phosphate, a novel metabolite to S. cerevisiae, which is then cleaved to glycolaldehyde and dihydroxyacetone phosphate. This synthetic pathway served as a platform for the biosynthesis of ethanol and ethylene glycol. The use of D-xylulose-1-phosphate as an entry point for xylose metabolism opens the way for optimizing chemical conversion of pentose sugars in S. cerevisiae in a modular fashion.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1627794

Journal Information:: PLoS ONE, Journal Name: PLoS ONE Journal Issue: 6 Vol. 11; ISSN 1932-6203

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

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