Inter-Species Cross-Seeding: Stability and Assembly of Rat–Human Amylin Aggregates

Berhanu, Workalemahu M.; Hansmann, Ulrich H.  E.

doi:10.1371/journal.pone.0097051

Inter-Species Cross-Seeding: Stability and Assembly of Rat–Human Amylin Aggregates

Journal Article · Thu May 08 00:00:00 EDT 2014 · PLoS ONE

DOI:https://doi.org/10.1371/journal.pone.0097051· OSTI ID:1627699

Berhanu, Workalemahu M. ^[1]; Hansmann, Ulrich H. E. ^[2]

University of Oklahoma, Norman, OK (United States); DOE/OSTI
University of Oklahoma, Norman, OK (United States)

Diseases such as type 2 diabetes, Alzheimer’s and Parkinson’s share as common feature the accumulation of mis-folded disease-specific protein aggregates into fibrillar structures, or plaques. These fibrils may either be toxic by themselves, or act as reservoirs for smaller cytotoxic oligomers. This suggests to investigate molecules as potential therapeutics that either reduce fibril formation or increase fibril stability. One example is rat amylin, which can inhibit aggregation of human amylin, a hallmark of type 2 diabetes. In the present paper, we use molecular dynamics to compare the stability of various preformed aggregates, built out of either human amylin, rat amylin, or mixtures of both. We considered two types of fibril-like oligomers: a single-layer in-register conformation, and a double-layer conformation in which the first U-shaped layer consists of rat amylin and the second layer of human amylin. Our results explain the weak amyloid-inhibiting properties of rat amylin and suggest that membrane leakage due to pore formation is responsible for the toxicity of rat amylin observed in a recent experiment. Together, our results put in question the use of rat amylin or the similar FDA approved drug pramlintide as an inhibitor of human amylin aggregation. They also point to mixed human-rat amylin fibril-like oligomers as possible model-systems for studies of amyloid formation that involve cross-species transmission.

View Accepted Manuscript (DOE)

Research Organization:: University of Oklahoma, Norman, OK (United States)

Sponsoring Organization:: National Institutes of Health (NIH); USDOE Office of Science (SC)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1627699

Journal Information:: PLoS ONE, Journal Name: PLoS ONE Journal Issue: 5 Vol. 9; ISSN 1932-6203

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

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