Flow Measurements in a Blood-Perfused Collagen Vessel Using X-Ray Micro-Particle Image Velocimetry

Antoine, Elizabeth; Buchanan, Cara; Fezzaa, Kamel; Lee, Wah-Keat; Rylander, M. Nichole; Vlachos, Pavlos

doi:10.1371/journal.pone.0081198

Flow Measurements in a Blood-Perfused Collagen Vessel Using X-Ray Micro-Particle Image Velocimetry

Journal Article · Sun Nov 17 23:00:00 EST 2013 · PLoS ONE

DOI:https://doi.org/10.1371/journal.pone.0081198· OSTI ID:1627663

Antoine, Elizabeth ^[1]; Buchanan, Cara ^[2]; Fezzaa, Kamel ^[3]; Lee, Wah-Keat ^[3]; Rylander, M. Nichole ^[4]; Vlachos, Pavlos ^[5]

Virginia Polytechnic Inst. and State Univ. (Virginia Tech), Blacksburg, VA (United States). Dept. of Mechanical Engineering; DOE/OSTI
Virginia Polytechnic Inst. and State Univ. (Virginia Tech), Blacksburg, VA (United States). WFU School of Biomedical Engineering and Sciences
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Virginia Polytechnic Inst. and State Univ. (Virginia Tech), Blacksburg, VA (United States). Dept. of Mechanical Engineering; Virginia Polytechnic Inst. and State Univ. (Virginia Tech), Blacksburg, VA (United States). WFU School of Biomedical Engineering and Sciences
Purdue Univ., West Lafayette, IN (United States). School of Mechanical Engineering

Blood-perfused tissue models are joining the emerging field of tumor engineering because they provide new avenues for modulation of the tumor microenvironment and preclinical evaluation of the therapeutic potential of new treatments. The characterization of fluid flow parameters in such in-vitro perfused tissue models is a critical step towards better understanding and manipulating the tumor microenvironment. However, traditional optical flow measurement methods are inapplicable because of the opacity of blood and the thickness of the tissue sample. In order to overcome the limitations of optical method we demonstrate the feasibility of using phase-contrast x-ray imaging to perform microscale particle image velocimetry (PIV) measurements of flow in blood perfused hydrated tissue-representative microvessels. However, phase contrast x-ray images significantly depart from the traditional PIV image paradigm, as they have high intensity background, very low signal-to-noise ratio, and volume integration effects. Hence, in order to achieve accurate measurements special attention must be paid to the image processing and PIV cross-correlation methodologies. Therefore we develop and demonstrate a methodology that incorporates image preprocessing as well as advanced PIV crosscorrelation methods to result in measured velocities within experimental uncertainty.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Lab. (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1627663

Journal Information:: PLoS ONE, Journal Name: PLoS ONE Journal Issue: 11 Vol. 8; ISSN 1932-6203

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

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