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Tetraspanins CD81 and CD82 Facilitate α4β1-Mediated Adhesion of Human Erythroblasts to Vascular Cell Adhesion Molecule-1

Journal Article · · PLoS ONE
 [1];  [2];  [2];  [3];  [2];  [4];  [2]
  1. Bristol Institute for Transfusion Sciences, Bristol (United Kingdom); DOE/OSTI
  2. Bristol Institute for Transfusion Sciences, Bristol (United Kingdom)
  3. Bristol University (United Kingdom)
  4. Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
+ Show Author Affiliations

The proliferation and terminal differentiation of erythroid progenitors occurs in human bone marrow within erythroblastic islands, specialised structures consisting of a central macrophage surrounded by developing erythroid cells. Many cell-cell and cell-matrix adhesive interactions maintain and regulate the co-ordinated daily production of reticulocytes. Erythroid cells express only one integrin, α4β1, throughout differentiation, and its interactions with both macrophage Vascular Cell Adhesion Molecule-1 and with extracellular matrix fibronectin are critical for erythropoiesis. We observed that proerythroblasts expressed a broad tetraspanin phenotype, and investigated whether any tetraspanin could modulate integrin function. A specific association between α4βb1 and CD81, CD82 and CD151 was demonstrated by confocal microscopy and co-immune precipitation. We observed that antibodies to CD81 and CD82 augmented adhesion of proerythroblasts to Vascular Cell Adhesion Molecule-1 but not to the fibronectin spliceoforms FnIII12-IIICS-15 and FnIII12–15. In contrast, different anti-CD151 antibodies augmented or inhibited adhesion of proerythroblasts to Vascular Cell Adhesion Molecule-1 and the fibronectin spliceoform FnIII12-IIICS-15 but not to FnIII12–15. These results strongly suggest that tetraspanins have a functional role in terminal erythropoiesis by modulating interactions of erythroblast α4β1 with both macrophages and extracellular matrix.

Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER); National Institute for Health Research; Department of Health (England); Biological and Biotechnology Research Council; National Institutes of Health (NIH)
Grant/Contract Number:
AC02-05CH11231
OSTI ID:
1627607
Journal Information:
PLoS ONE, Journal Name: PLoS ONE Journal Issue: 5 Vol. 8; ISSN 1932-6203
Publisher:
Public Library of ScienceCopyright Statement
Country of Publication:
United States
Language:
English

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Cited By (6)

Macrophage heterogeneity in tissues: phenotypic diversity and functions journal October 2014
Tspan8 and Tspan8/CD151 knockout mice unravel the contribution of tumor and host exosomes to tumor progression journal December 2018
The Activation and Signaling Mechanisms of GPR56/ADGRG1 in Melanoma Cell journal August 2018
Beyond the Matrix: The Many Non-ECM Ligands for Integrins journal February 2018
Cellular dynamics of mammalian red blood cell production in the erythroblastic island niche journal August 2019
HIV-1 Nef and Vpu Are Functionally Redundant Broad-Spectrum Modulators of Cell Surface Receptors, Including Tetraspanins journal December 2014

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
cell adhesion
cell binding
cloning
erythroblasts
erythropoiesis
integrins
macrophages
reticulocytes