Tetramerization Reinforces the Dimer Interface of MnSOD

Sheng, Yuewei; Durazo, Armando; Schumacher, Mikhail; Gralla, Edith Butler; Cascio, Duilio; Cabelli, Diane E.; Valentine, Joan Selverstone

doi:10.1371/journal.pone.0062446

Tetramerization Reinforces the Dimer Interface of MnSOD

Journal Article · Tue May 07 00:00:00 EDT 2013 · PLoS ONE

DOI:https://doi.org/10.1371/journal.pone.0062446· OSTI ID:1627605

Sheng, Yuewei ^[1]; Durazo, Armando ^[2]; Schumacher, Mikhail ^[3]; Gralla, Edith Butler ^[3]; Cascio, Duilio ^[4]; Cabelli, Diane E. ^[5]; Valentine, Joan Selverstone ^[6]

Univ. of California, Los Angeles, CA (United States). Dept. of Chemistry and Biochemistry; DOE/OSTI
Univ. of California, Los Angeles, CA (United States). Dept. of Chemistry and Biochemistry; Univ. of Arizona, Tucson, AZ (United States). Dept. of Chemical and Environmental Engineering
Univ. of California, Los Angeles, CA (United States). Dept. of Chemistry and Biochemistry
Univ. of California, Los Angeles, CA (United States). Dept. of Energy-Inst. for Genomics and Proteomics
Brookhaven National Lab. (BNL), Upton, NY (United States)
Univ. of California, Los Angeles, CA (United States). Dept. of Chemistry and Biochemistry; Ewha Womans Univ., Seoul (Korea). Dept. of Bioinspired Science

Two yeast manganese superoxide dismutases (MnSOD), one from Saccharomyces cerevisiae mitochondria (ScMnSOD) and the other from Candida albicans cytosol (CaMnSODc), have most biochemical and biophysical properties in common, yet ScMnSOD is a tetramer and CaMnSODc is a dimer or ‘‘loose tetramer’’ in solution. Although CaMnSODc was found to crystallize as a tetramer, there is no indication from the solution properties that the functionality of CaMnSODc in vivo depends upon the formation of the tetrameric structure. To elucidate further the functional significance of MnSOD quaternary structure, wild-type and mutant forms of ScMnSOD (K182R, A183P mutant) and CaMnSODc (K184R, L185P mutant) with the substitutions at dimer interfaces were analyzed with respect to their oligomeric states and resistance to pH, heat, and denaturant. Dimeric CaMnSODc was found to be significantly more subject to thermal or denaturant-induced unfolding than tetrameric ScMnSOD. The residue substitutions at dimer interfaces caused dimeric CaMnSODc but not tetrameric ScMnSOD to dissociate into monomers. We conclude that the tetrameric assembly strongly reinforces the dimer interface, which is critical for MnSOD activity.

View Accepted Manuscript (DOE)

Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division

Grant/Contract Number:: AC02-98CH10886

OSTI ID:: 1627605

Journal Information:: PLoS ONE, Journal Name: PLoS ONE Journal Issue: 5 Vol. 8; ISSN 1932-6203

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

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