A New Genetic Linkage Map of the Zygomycete Fungus Phycomyces blakesleeanus

Chaudhary, Suman; Polaino, Silvia; Shakya, Viplendra P.  S.; Idnurm, Alexander

doi:10.1371/journal.pone.0058931

A New Genetic Linkage Map of the Zygomycete Fungus Phycomyces blakesleeanus

Journal Article · Thu Mar 14 00:00:00 EDT 2013 · PLoS ONE

DOI:https://doi.org/10.1371/journal.pone.0058931· OSTI ID:1627590

Chaudhary, Suman ^[1]; Polaino, Silvia ^[2]; Shakya, Viplendra P. S. ^[2]; Idnurm, Alexander ^[2]

University of Missouri, Kansas City, MO (United States); DOE/OSTI
University of Missouri, Kansas City, MO (United States)

Phycomyces blakesleeanus is a member of the subphylum Mucoromycotina. A genetic map was constructed from 121 progeny of a cross between two wild type isolates of P. blakesleeanus with 134 markers. The markers were mostly PCRRFLPs. Markers were located on 46 scaffolds of the genome sequence, covering more than 97% of the genome. Analysis of the alleles in the progeny revealed nine or 12 linkage groups, depending on the log of the odds (LOD) score, across 1583.4 cM at LOD 5. The linkage groups were overlaid on previous mapping data from crosses between mutants, aided by new identification of the mutations in primary metabolism mutant strains. The molecular marker map, the phenotype map and the genome sequence are overall congruent, with some exceptions. The new genetic map provides a genome-wide estimate for recombination, with the average of 33.2 kb per cM. This frequency is one piece of evidence for meiosis during zygospore development in Mucoromycotina species. At the same time as meiosis, transmission of non-recombinant chromosomes is also evident in the mating process in Phycomyces. The new map provides scaffold ordering for the genome sequence and a platform upon which to identify the genes in mutants that are affected in traits of interest, such as carotene biosynthesis, phototropism or gravitropism, using positional cloning.

View Accepted Manuscript (DOE)

Research Organization:: Joint Genome Institute (JGI), Berkeley, CA (United States)

Sponsoring Organization:: National Science Foundation (NSF); USDOE; University of Missouri

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1627590

Journal Information:: PLoS ONE, Journal Name: PLoS ONE Journal Issue: 3 Vol. 8; ISSN 1932-6203

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

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A New Genetic Linkage Map of the Zygomycete Fungus Phycomyces blakesleeanus

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