Univ. of California, Berkeley, CA (United States). Biophysics Graduate Group; DOE/OSTI
Univ. of Alberta, Edmonton, AB (Canada). Dept. of Chemistry
Max Planck Inst. for Biophysical Chemistry, Gottingen (Germany). Dept. of NanoBiophotonics
Eidgenossische Technische Hochschule (ETH) Zurich, (Switzerland). Inst. of Biochemistry
Florida State Univ., Tallahassee, FL (United States). National High Magnetic Field Lab. (MagLab); Univ. of California, Berkeley, CA (United States). Dept. of Physics
Univ. of California, Berkeley, CA (United States). Biophysics Graduate Group; Univ. of California, Berkeley, CA (United States). California Inst. for Quantitative Biosciences (QB3); Univ. of California, Berkeley, CA (United States). Bay Area Physical Sciences. Oncology Center; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Physical Biosciences Division
Recent advances in fluorescence microscopy have extended the spatial resolution to the nanometer scale. Here, we report an engineered photoconvertible fluorescent protein (pcFP) variant, designated as mMaple, that is suited for use in multiple conventional and super-resolution imaging modalities, specifically, widefield and confocal microscopy, structured illumination microscopy (SIM), and single-molecule localization microscopy. We demonstrate the versatility of mMaple by obtaining super-resolution images of protein organization in Escherichia coli and conventional fluorescence images of mammalian cells. Beneficial features of mMaple include high photostability of the green state when expressed in mammalian cells and high steady state intracellular protein concentration of functional protein when expressed in E. coli. mMaple thus enables both fast live-cell ensemble imaging and high precision single molecule localization for a single pcFPcontaining construct.
McEvoy, Ann L., et al. "mMaple: A Photoconvertible Fluorescent Protein for Use in Multiple Imaging Modalities." PLoS ONE, vol. 7, no. 12, Dec. 2012. https://doi.org/10.1371/journal.pone.0051314
McEvoy, Ann L., Hoi, Hiofan, Bates, Mark, Platonova, Evgenia, Cranfill, Paula J., Baird, Michelle A., Davidson, Michael W., Ewers, Helge, Liphardt, Jan, Campbell, Robert E., & Jones, D. Dafydd (2012). mMaple: A Photoconvertible Fluorescent Protein for Use in Multiple Imaging Modalities. PLoS ONE, 7(12). https://doi.org/10.1371/journal.pone.0051314
McEvoy, Ann L., Hoi, Hiofan, Bates, Mark, et al., "mMaple: A Photoconvertible Fluorescent Protein for Use in Multiple Imaging Modalities," PLoS ONE 7, no. 12 (2012), https://doi.org/10.1371/journal.pone.0051314
@article{osti_1627567,
author = {McEvoy, Ann L. and Hoi, Hiofan and Bates, Mark and Platonova, Evgenia and Cranfill, Paula J. and Baird, Michelle A. and Davidson, Michael W. and Ewers, Helge and Liphardt, Jan and Campbell, Robert E. and others},
title = {mMaple: A Photoconvertible Fluorescent Protein for Use in Multiple Imaging Modalities},
annote = {Recent advances in fluorescence microscopy have extended the spatial resolution to the nanometer scale. Here, we report an engineered photoconvertible fluorescent protein (pcFP) variant, designated as mMaple, that is suited for use in multiple conventional and super-resolution imaging modalities, specifically, widefield and confocal microscopy, structured illumination microscopy (SIM), and single-molecule localization microscopy. We demonstrate the versatility of mMaple by obtaining super-resolution images of protein organization in Escherichia coli and conventional fluorescence images of mammalian cells. Beneficial features of mMaple include high photostability of the green state when expressed in mammalian cells and high steady state intracellular protein concentration of functional protein when expressed in E. coli. mMaple thus enables both fast live-cell ensemble imaging and high precision single molecule localization for a single pcFPcontaining construct.},
doi = {10.1371/journal.pone.0051314},
url = {https://www.osti.gov/biblio/1627567},
journal = {PLoS ONE},
issn = {ISSN 1932-6203},
number = {12},
volume = {7},
place = {United States},
publisher = {Public Library of Science},
year = {2012},
month = {12}}
11th European Quantum Electronics Conference (CLEO/EQEC), CLEO/Europe - EQEC 2009 - European Conference on Lasers and Electro-Optics and the European Quantum Electronics Conferencehttps://doi.org/10.1109/cleoe-eqec.2009.5191624