Sequence Variants of the Phytophthora sojae RXLR Effector Avr3a/5 Are Differentially Recognized by Rps3a and Rps5 in Soybean

Dong, Suomeng; Yu, Dan; Cui, Linkai; Qutob, Dinah; Tedman-Jones, Jennifer; Kale, Shiv D.; Tyler, Brett M.; Wang, Yuanchao; Gijzen, Mark

doi:10.1371/journal.pone.0020172

Sequence Variants of the Phytophthora sojae RXLR Effector Avr3a/5 Are Differentially Recognized by Rps3a and Rps5 in Soybean

Journal Article · Thu Jul 14 00:00:00 EDT 2011 · PLoS ONE

DOI:https://doi.org/10.1371/journal.pone.0020172· OSTI ID:1627461

Dong, Suomeng ^[1]; Yu, Dan ^[2]; Cui, Linkai ^[3]; Qutob, Dinah ^[4]; Tedman-Jones, Jennifer ^[4]; Kale, Shiv D. ^[5]; Tyler, Brett M. ^[5]; Wang, Yuanchao ^[3]; Gijzen, Mark ^[4]

Agriculture and Agri-Food Canada, London, ON (Canada); Nanjing Agricultural Univ., Nanjing (China). Dept. of Plant Pathology; DOE/OSTI
Agriculture and Agri-Food Canada, London, ON (Canada); Nanjing Agricultural Univ., Nanjing (China). Dept. of Plant Pathology
Nanjing Agricultural Univ., Nanjing (China). Dept. of Plant Pathology
Agriculture and Agri-Food Canada, London, ON (Canada)
Virginia Polytechnic Inst. and State Univ. (Virginia Tech), Blacksburg, VA (United States). Virginia Bioinformatics Inst.

The perception of Phytophthora sojae avirulence (Avr) gene products by corresponding soybean resistance (Rps) gene products causes effector triggered immunity. Past studies have shown that the Avr3a and Avr5 genes of P. sojae are genetically linked, and the Avr3a gene encoding a secreted RXLR effector protein was recently identified. We now provide evidence that Avr3a and Avr5 are allelic. Genetic mapping data from F2 progeny indicates that Avr3a and Avr5 co-segregate, and haplotype analysis of P. sojae strain collections reveal sequence and transcriptional polymorphisms that are consistent with a single genetic locus encoding Avr3a/5. Transformation of P. sojae and transient expression in soybean were performed to test how Avr3a/5 alleles interact with soybean Rps3a and Rps5. Over-expression of Avr3a/5 in a P. sojae strain that is normally virulent on Rps3a and Rps5 results in avirulence to Rps3a and Rps5; whereas silencing of Avr3a/5 causes gain of virulence in a P. sojae strain that is normally avirulent on Rps3a and Rps5 soybean lines. Transient expression and cobombardment with a reporter gene confirms that Avr3a/5 triggers cell death in Rps5 soybean leaves in an appropriate allelespecific manner. Sequence analysis of the Avr3a/5 gene identifies crucial residues in the effector domain that distinguish recognition by Rps3a and Rps5.

View Accepted Manuscript (DOE)

Research Organization:: Agriculture and Agri-Food Canada, London, ON (Canada)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Earth and Environmental Systems Science Division; Agriculture and Agri-Food Canada (AAFC) Crop Genomics Initiative; National Natural Science Foundation of China; USDA National Institute for Food and Agriculture; National Science Foundation (NSF)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1627461

Journal Information:: PLoS ONE, Journal Name: PLoS ONE Journal Issue: 7 Vol. 6; ISSN 1932-6203

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

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