Heterochromatic Genome Stability Requires Regulators of Histone H3 K9 Methylation

Peng, Jamy C.; Karpen, Gary H.

doi:10.1371/journal.pgen.1000435

Heterochromatic Genome Stability Requires Regulators of Histone H3 K9 Methylation

Journal Article · Fri Mar 27 00:00:00 EDT 2009 · PLoS Genetics

DOI:https://doi.org/10.1371/journal.pgen.1000435· OSTI ID:1627274

Peng, Jamy C. ^[1]; Karpen, Gary H. ^[2]

Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Univ. of California, Berkeley, CA (United States). Dept. of Genome and Computational Biology; Univ. of California, Berkeley, CA (United States). Dept. of Molecular and Cell Biology; DOE/OSTI
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Univ. of California, Berkeley, CA (United States). Dept. of Genome and Computational Biology; Univ. of California, Berkeley, CA (United States). Dept. of Molecular and Cell Biology

Heterochromatin contains many repetitive DNA elements and few protein-encoding genes, yet it is essential for chromosome organization and inheritance. Here, we show that Drosophila that lack the Su(var)3-9 H3K9 methyltransferase display significantly elevated frequencies of spontaneous DNA damage in heterochromatin, in both somatic and germ-line cells. Accumulated DNA damage in these mutants correlates with chromosomal defects, such as translocations and loss of heterozygosity. DNA repair and mitotic checkpoints are also activated in mutant animals and are required for their viability. Similar effects of lower magnitude were observed in animals that lack the RNA interference pathway component Dcr2. These results suggest that the H3K9 methylation and RNAi pathways ensure heterochromatin stability.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). National Energy Research Scientific Computing Center (NERSC)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1627274

Journal Information:: PLoS Genetics, Journal Name: PLoS Genetics Journal Issue: 3 Vol. 5; ISSN 1553-7404

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

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