Protein Modularity of Alternatively Spliced Exons Is Associated with Tissue-Specific Regulation of Alternative Splicing

Xing, Yi; Lee, Christopher J.

doi:10.1371/journal.pgen.0010034

Protein Modularity of Alternatively Spliced Exons Is Associated with Tissue-Specific Regulation of Alternative Splicing

Journal Article · Fri Sep 16 00:00:00 EDT 2005 · PLoS Genetics

DOI:https://doi.org/10.1371/journal.pgen.0010034· OSTI ID:1627262

Xing, Yi ^[1]; Lee, Christopher J. ^[2]

Univ. of California, Los Angeles, CA (United States). Dept. of Chemsitry and Biochemistry. Molecular Biology Inst. Inst. for Genomics and Proteomics; DOE/OSTI
Univ. of California, Los Angeles, CA (United States). Dept. of Chemsitry and Biochemistry. Molecular Biology Inst. Inst. for Genomics and Proteomics

Recent comparative genomic analysis of alternative splicing has shown that protein modularity is an important criterion for functional alternative splicing events. Exons that are alternatively spliced in multiple organisms are much more likely to be an exact multiple of 3 nt in length, representing a class of ‘‘modular’’ exons that can be inserted or removed from the transcripts without affecting the rest of the protein. To understand the precise roles of these modular exons, in this paper we have analyzed microarray data for 3,126 alternatively spliced exons across ten mouse tissues generated by Pan and coworkers. We show that modular exons are strongly associated with tissue-specific regulation of alternative splicing. Exons that are alternatively spliced at uniformly high transcript inclusion levels or uniformly low levels show no preference for protein modularity. In contrast, alternatively spliced exons with dramatic changes of inclusion levels across mouse tissues (referred to as ‘‘tissue-switched’’ exons) are both strikingly biased to be modular and are strongly conserved between human and mouse. The analysis of different subsets of tissue switched exons shows that the increased protein modularity cannot be explained by the overall exon inclusion level, but is specifically associated with tissue-switched alternative splicing.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of California, Los Angeles, CA (United States)

Sponsoring Organization:: National Institutes of Health (NIH); USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

Grant/Contract Number:: FC02-02ER63421

OSTI ID:: 1627262

Journal Information:: PLoS Genetics, Journal Name: PLoS Genetics Journal Issue: 3 Vol. 1; ISSN 1553-7404

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

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