Stepwise Translocation of Dpo4 Polymerase during Error-Free Bypass of an oxoG Lesion

Rechkoblit, Olga; Malinina, Lucy; Cheng, Yuan; Kuryavyi, Vitaly; Broyde, Suse; Geacintov, Nicholas E.; Patel, Dinshaw J.

doi:10.1371/journal.pbio.0040011

Stepwise Translocation of Dpo4 Polymerase during Error-Free Bypass of an oxoG Lesion

Journal Article · Mon Jan 02 23:00:00 EST 2006 · PLoS Biology (Online)

DOI:https://doi.org/10.1371/journal.pbio.0040011· OSTI ID:1627144

Rechkoblit, Olga ^[1]; Malinina, Lucy ^[2]; Cheng, Yuan ^[2]; Kuryavyi, Vitaly ^[2]; Broyde, Suse ^[3]; Geacintov, Nicholas E. ^[4]; Patel, Dinshaw J. ^[2]

Memorial Sloan-Kettering Cancer Center, New York, NY (United States); DOE/OSTI
Memorial Sloan-Kettering Cancer Center, New York, NY (United States)
New York University (NYU), NY (United States)
New York University (NYU), NY (United States). Chemistry Dept.

7,8-dihydro-8-oxoguanine (oxoG), the predominant lesion formed following oxidative damage of DNA by reactive oxygen species, is processed differently by replicative and bypass polymerases. Our kinetic primer extension studies demonstrate that the bypass polymerase Dpo4 preferentially inserts C opposite oxoG, and also preferentially extends from the oxoGC base pair, thus achieving error-free bypass of this lesion. We have determined the crystal structures of preinsertion binary, insertion ternary, and postinsertion binary complexes of oxoG-modified template-primer DNA and Dpo4. These structures provide insights into the translocation mechanics of the bypass polymerase during a complete cycle of nucleotide incorporation. Specifically, during noncovalent dCTP insertion opposite oxoG (or G), the little-finger domain–DNA phosphate contacts translocate by one nucleotide step, while the thumb domain–DNA phosphate contacts remain fixed. By contrast, during the nucleotidyl transfer reaction that covalently incorporates C opposite oxoG, the thumb-domain–phosphate contacts are translocated by one nucleotide step, while the little-finger contacts with phosphate groups remain fixed. These stepwise conformational transitions accompanying nucleoside triphosphate binding and covalent nucleobase incorporation during a full replication cycle of Dpo4-catalyzed bypass of the oxoG lesion are distinct from the translocation events in replicative polymerases.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE; National Institutes of Health (NIH)

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1627144

Journal Information:: PLoS Biology (Online), Journal Name: PLoS Biology (Online) Journal Issue: 1 Vol. 4; ISSN 1545-7885

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

References (47)

Switching from high-fidelity replicases to low-fidelity lesion-bypass polymerases Plosky, Brian S.; Woodgate, Roger Current Opinion in Genetics & Development, Vol. 14, Issue 2 https://doi.org/10.1016/j.gde.2004.02.002	journal	April 2004
Crystal Structure of the Catalytic Core of Human DNA Polymerase Kappa Uljon, Sacha N.; Johnson, Robert E.; Edwards, Thomas A. Structure, Vol. 12, Issue 8 https://doi.org/10.1016/j.str.2004.05.011	journal	August 2004
Structures of Mismatch Replication Errors Observed in a DNA Polymerase Johnson, Sean J.; Beese, Lorena S. Cell, Vol. 116, Issue 6 https://doi.org/10.1016/s0092-8674(04)00252-1	journal	March 2004
Damage repair DNA polymerases Y Yang, Wei Current Opinion in Structural Biology, Vol. 13, Issue 1 https://doi.org/10.1016/s0959-440x(02)00003-9	journal	February 2003
In Vitro Nucleotide Misinsertion Opposite the Oxidized Guanosine Lesions Spiroiminodihydantoin and Guanidinohydantoin and DNA Synthesis Past the Lesions Using Escherichia coli DNA Polymerase I (Klenow Fragment) ^† Kornyushyna, Olga; Berges, Aym M.; Muller, James G. Biochemistry, Vol. 41, Issue 51 https://doi.org/10.1021/bi0264925	journal	December 2002
Crystal Structures of Human DNA Polymerase β Complexed with Gapped and Nicked DNA: Evidence for an Induced Fit Mechanism ^† ^, ^‡ Sawaya, Michael R.; Prasad, Rajendra; Wilson, Samuel H. Biochemistry, Vol. 36, Issue 37 https://doi.org/10.1021/bi9703812	journal	September 1997
Steady-State and Pre-Steady-State Kinetic Analysis of 8-Oxo-7,8-dihydroguanosine Triphosphate Incorporation and Extension by Replicative and Repair DNA Polymerases ^† Einolf, Heidi J.; Schnetz-Boutaud, Nathalie; Guengerich, F. Peter Biochemistry, Vol. 37, Issue 38 https://doi.org/10.1021/bi981346d	journal	September 1998
Insertion of specific bases during DNA synthesis past the oxidation-damaged base 8-oxodG Shibutani, Shinya; Takeshita, Masaru; Grollman, Arthur P. Nature, Vol. 349, Issue 6308 https://doi.org/10.1038/349431a0	journal	January 1991
Efficient and accurate replication in the presence of 7,8-dihydro-8-oxoguanine by DNA polymerase η Haracska, Lajos; Yu, Sung-Lim; Johnson, Robert E. Nature Genetics, Vol. 25, Issue 4 https://doi.org/10.1038/78169	journal	August 2000
Replication of a cis–syn thymine dimer at atomic resolution Ling, Hong; Boudsocq, François; Plosky, Brian S. Nature, Vol. 424, Issue 6952 https://doi.org/10.1038/nature01919	journal	August 2003
Nucleotide insertion opposite a cis-syn thymine dimer by a replicative DNA polymerase from bacteriophage T7 Li, Ying; Dutta, Shuchismita; Doublié, Sylvie Nature Structural & Molecular Biology, Vol. 11, Issue 8 https://doi.org/10.1038/nsmb792	journal	July 2004
A closed conformation for the Pol λ catalytic cycle Garcia-Diaz, Miguel; Bebenek, Katarzyna; Krahn, Joseph M. Nature Structural & Molecular Biology, Vol. 12, Issue 1 https://doi.org/10.1038/nsmb876	journal	December 2004
Lesion (in)tolerance reveals insights into DNA replication fidelity Freisinger, Eva; Grollman, Arthur P.; Miller, Holly The EMBO Journal, Vol. 23, Issue 7 https://doi.org/10.1038/sj.emboj.7600158	journal	April 2004
Fidelity of Dpo4: effect of metal ions, nucleotide selection and pyrophosphorolysis Vaisman, Alexandra; Ling, Hong; Woodgate, Roger The EMBO Journal, Vol. 24, Issue 17 https://doi.org/10.1038/sj.emboj.7600786	journal	August 2005
Crystal structure of a benzo[a]pyrene diol epoxide adduct in a ternary complex with a DNA polymerase Ling, H.; Sayer, J. M.; Plosky, B. S. Proceedings of the National Academy of Sciences, Vol. 101, Issue 8 https://doi.org/10.1073/pnas.0308332100	journal	February 2004
Crystal structures of 2-acetylaminofluorene and 2-aminofluorene in complex with T7 DNA polymerase reveal mechanisms of mutagenesis Dutta, S.; Li, Y.; Johnson, D. Proceedings of the National Academy of Sciences, Vol. 101, Issue 46 https://doi.org/10.1073/pnas.0406516101	journal	November 2004
Processive DNA synthesis observed in a polymerase crystal suggests a mechanism for the prevention of frameshift mutations Johnson, S. J.; Taylor, J. S.; Beese, L. S. Proceedings of the National Academy of Sciences, Vol. 100, Issue 7 https://doi.org/10.1073/pnas.0630532100	journal	March 2003
Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4): an archaeal DinB-like DNA polymerase with lesion-bypass properties akin to eukaryotic poleta Boudsocq, F. Nucleic Acids Research, Vol. 29, Issue 22 https://doi.org/10.1093/nar/29.22.4607	journal	November 2001
Accuracy, lesion bypass, strand displacement and translocation by DNA polymerases Steitz, Thomas A.; Yin, Y. Whitney Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences, Vol. 359, Issue 1441 https://doi.org/10.1098/rstb.2003.1374	journal	January 2004
Combined pseudo-merohedral twinning, non-crystallographic symmetry and pseudo-translation in a monoclinic crystal form of the γδ T-cell ligand T10 Rudolph, Markus G.; Wingren, Christer; Crowley, Michael P. Acta Crystallographica Section D Biological Crystallography, Vol. 60, Issue 4 https://doi.org/10.1107/s0907444904002239	journal	March 2004
Conformational Coupling in DNA Polymerase Fidelity Johnson, Kenneth A. Annual Review of Biochemistry, Vol. 62, Issue 1 https://doi.org/10.1146/annurev.bi.62.070193.003345	journal	June 1993
EUKARYOTIC TRANSLESION SYNTHESIS DNA POLYMERASES: Specificity of Structure and Function Prakash, Satya; Johnson, Robert E.; Prakash, Louise Annual Review of Biochemistry, Vol. 74, Issue 1 https://doi.org/10.1146/annurev.biochem.74.082803.133250	journal	June 2005
Systematic analysis of domain motions in proteins from conformational change: New results on citrate synthase and T4 lysozyme Hayward, Steven; Berendsen, Herman J. C. Proteins: Structure, Function, and Genetics, Vol. 30, Issue 2 https://doi.org/10.1002/(SICI)1097-0134(19980201)30:2<144::AID-PROT4>3.0.CO;2-N	journal	February 1998
[19] Gel fidelity assay measuring nucleotide misinsertion, exonucleolytic proofreading, and lesion bypass efficiencies Creighton, Steven; Bloom, Linda B.; Goodman, Myron F. Methods in Enzymology https://doi.org/10.1016/0076-6879(95)62021-4	book	January 1995
Crystal Structure of a Y-Family DNA Polymerase in Action Ling, Hong; Boudsocq, François; Woodgate, Roger Cell, Vol. 107, Issue 1 https://doi.org/10.1016/S0092-8674(01)00515-3	journal	October 2001
Yeast DNA Polymerase η Utilizes an Induced-Fit Mechanism of Nucleotide Incorporation Washington, M. Todd; Prakash, Louise; Prakash, Satya Cell, Vol. 107, Issue 7 https://doi.org/10.1016/S0092-8674(01)00613-4	journal	December 2001
Structures of Mismatch Replication Errors Observed in a DNA Polymerase Johnson, Sean J.; Beese, Lorena S. Cell, Vol. 116, Issue 6 https://doi.org/10.1016/S0092-8674(04)00252-1	journal	March 2004
Damage repair DNA polymerases Y Yang, Wei Current Opinion in Structural Biology, Vol. 13, Issue 1 https://doi.org/10.1016/S0959-440X(02)00003-9	journal	February 2003
Structure of DNA Polymerase β with the Mutagenic DNA Lesion 8-Oxodeoxyguanine Reveals Structural Insights into Its Coding Potential Krahn, Joseph M.; Beard, William A.; Miller, Holly Structure, Vol. 11, Issue 1 https://doi.org/10.1016/S0969-2126(02)00930-9	journal	January 2003
Structure of the Catalytic Core of S. cerevisiae DNA Polymerase η Trincao, Jose; Johnson, Robert E.; Escalante, Carlos R. Molecular Cell, Vol. 8, Issue 2 https://doi.org/10.1016/S1097-2765(01)00306-9	journal	August 2001
Crystal Structure of a DinB Lesion Bypass DNA Polymerase Catalytic Fragment Reveals a Classic Polymerase Catalytic Domain Zhou, Bo-Lu; Pata, Janice D.; Steitz, Thomas A. Molecular Cell, Vol. 8, Issue 2 https://doi.org/10.1016/S1097-2765(01)00310-0	journal	August 2001
Snapshots of Replication through an Abasic Lesion Ling, Hong; Boudsocq, François; Woodgate, Roger Molecular Cell, Vol. 13, Issue 5 https://doi.org/10.1016/S1097-2765(04)00101-7	journal	March 2004
Trading Places: How Do DNA Polymerases Switch during Translesion DNA Synthesis? Friedberg, Errol C.; Lehmann, Alan R.; Fuchs, Robert P. P. Molecular Cell, Vol. 18, Issue 5 https://doi.org/10.1016/j.molcel.2005.03.032	journal	May 2005
Oxidative damage to DNA: formation, measurement and biochemical features Cadet, J. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, Vol. 531, Issue 1-2 https://doi.org/10.1016/j.mrfmmm.2003.09.001	journal	October 2003
Mechanism of DNA Polymerization Catalyzed by Sulfolobus solfataricus P2 DNA Polymerase IV ^† Fiala, Kevin A.; Suo, Zucai Biochemistry, Vol. 43, Issue 7 https://doi.org/10.1021/bi035746z	journal	February 2004
Analysis of Nucleotide Insertion and Extension at 8-Oxo-7,8-dihydroguanine by Replicative T7 Polymerase exo ^- and Human Immunodeficiency Virus-1 Reverse Transcriptase Using Steady-State and Pre-Steady-State Kinetics ^† Furge, Laura Lowe; Guengerich, F. Peter Biochemistry, Vol. 36, Issue 21 https://doi.org/10.1021/bi9627267	journal	May 1997
Replication by human DNA polymerase-ι occurs by Hoogsteen base-pairing Nair, Deepak T.; Johnson, Robert E.; Prakash, Satya Nature, Vol. 430, Issue 6997 https://doi.org/10.1038/nature02692	journal	July 2004
Error-prone replication of oxidatively damaged DNA by a high-fidelity DNA polymerase Hsu, Gerald W.; Ober, Matthias; Carell, Thomas Nature, Vol. 431, Issue 7005 https://doi.org/10.1038/nature02908	journal	August 2004
Dpo4 is hindered in extending a G·T mismatch by a reverse wobble Trincao, Jose; Johnson, Robert E.; Wolfle, William T. Nature Structural & Molecular Biology, Vol. 11, Issue 5 https://doi.org/10.1038/nsmb755	journal	April 2004
Structural basis for the dual coding potential of 8-oxoguanosine by a high-fidelity DNA polymerase Brieba, Luis G.; Eichman, Brandt F.; Kokoska, Robert J. The EMBO Journal, Vol. 23, Issue 17 https://doi.org/10.1038/sj.emboj.7600354	journal	August 2004
Review article: chronic inflammation and reactive oxygen and nitrogen metabolism - implications in DNA damage and mutagenesis: REVIEW: CHRONIC INFLAMMATION and MUTAGENESIS Grisham, M. B.; Jourd'Heuil, D.; Wink, D. A. Alimentary Pharmacology & Therapeutics, Vol. 14 https://doi.org/10.1046/j.1365-2036.2000.014s1003.x	journal	February 2000
“Action-at-a-Distance” Mutagenesis Efrati, Edna; Tocco, Georges; Eritja, Ramon Journal of Biological Chemistry, Vol. 274, Issue 22 https://doi.org/10.1074/jbc.274.22.15920	journal	May 1999
Fidelity of Nucleotide Insertion at 8-Oxo-7,8-dihydroguanine by Mammalian DNA Polymerase δ Einolf, Heidi J.; Guengerich, F. Peter Journal of Biological Chemistry, Vol. 276, Issue 6 https://doi.org/10.1074/jbc.M006696200	journal	February 2001
DNA Adduct Bypass Polymerization by Sulfolobus solfataricus DNA Polymerase Dpo4 : ANALYSIS AND CRYSTAL STRUCTURES OF MULTIPLE BASE PAIR SUBSTITUTION AND FRAMESHIFT PRODUCTS WITH THE ADDUCT 1, Zang, Hong; Goodenough, Angela K.; Choi, Jeong-Yun Journal of Biological Chemistry, Vol. 280, Issue 33 https://doi.org/10.1074/jbc.M504756200	journal	June 2005
AMoRe : an automated package for molecular replacement Navaza, J. Acta Crystallographica Section A Foundations of Crystallography, Vol. 50, Issue 2 https://doi.org/10.1107/S0108767393007597	journal	March 1994
Combined pseudo-merohedral twinning, non-crystallographic symmetry and pseudo-translation in a monoclinic crystal form of the γδ T-cell ligand T10 Rudolph, Markus G.; Wingren, Christer; Crowley, Michael P. Acta Crystallographica Section D Biological Crystallography, Vol. 60, Issue 4 https://doi.org/10.1107/S0907444904002239	journal	March 2004
Refinement of Macromolecular Structures by the Maximum-Likelihood Method Murshudov, G. N.; Vagin, A. A.; Dodson, E. J. Acta Crystallographica Section D Biological Crystallography, Vol. 53, Issue 3 https://doi.org/10.1107/S0907444996012255	journal	May 1997

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