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Quantifying engineered nanomaterial toxicity: comparison of common cytotoxicity and gene expression measurements

Journal Article · · Journal of Nanobiotechnology
 [1];  [2];  [3];  [4];  [5];  [6];  [7];  [6]
  1. National Inst. of Standards and Technology (NIST), Gaithersburg, MD (United States). Biosystems and Biomaterials Division; DOE/OSTI
  2. Los Alamos National Lab. (LANL), Los Alamos, NM (United States). Bioscience Division
  3. Los Alamos National Lab. (LANL), Los Alamos, NM (United States). Center for Integrated Nanotechnologies, Materials Physics & Applications Division
  4. Los Alamos National Lab. (LANL), Los Alamos, NM (United States). Center for Integrated Nanotechnologies, Materials Physics & Applications Division; Boston Univ., MA (United States). Department of Biomedical Engineering and Division of Materials Science and Engineering
  5. Los Alamos National Lab. (LANL), Los Alamos, NM (United States). Center for Integrated Nanotechnologies, Materials Physics & Applications Division
  6. National Inst. of Standards and Technology (NIST), Gaithersburg, MD (United States). Biosystems and Biomaterials Division
  7. Los Alamos National Lab. (LANL), Los Alamos, NM (United States). Defense Systems and Analysis Division
+ Show Author Affiliations
When evaluating the toxicity of engineered nanomaterials (ENMS) it is important to use multiple bioassays based on different mechanisms of action. In this regard we evaluated the use of gene expression and common cytotoxicity measurements using as test materials, two selected nanoparticles with known differences in toxicity, 5 nm mercaptoundecanoic acid (MUA)-capped InP and CdSe quantum dots (QDs). We tested the effects of these QDs at concentrations ranging from 0.5 to 160 µg/mL on cultured normal human bronchial epithelial (NHBE) cells using four common cytotoxicity assays: the dichlorofluorescein assay for reactive oxygen species (ROS), the lactate dehydrogenase assay for membrane viability (LDH), the mitochondrial dehydrogenase assay for mitochondrial function, and the Comet assay for DNA strand breaks.
Research Organization:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Sponsoring Organization:
USDOE Office of Science (SC)
Grant/Contract Number:
AC52-06NA25396
OSTI ID:
1626901
Journal Information:
Journal of Nanobiotechnology, Journal Name: Journal of Nanobiotechnology Journal Issue: 1 Vol. 15; ISSN 1477-3155
Publisher:
BioMed CentralCopyright Statement
Country of Publication:
United States
Language:
English

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Cited By (1)

Targeting and non-targeting effects of nanomaterials on DNA: challenges and perspectives journal August 2019

Figures / Tables (6)

Fig. 1(p. 2)figure Fig. 1
Fig. 2(p. 3)figure Fig. 2
Fig. 3(p. 3)figure Fig. 3
Fig. 4(p. 4)figure Fig. 4
Table 1(p. 5)table Table 1
Table 2(p. 6)table Table 2

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Biomarkers
Comet assay
Cytotoxicity
Gene expression
Genotoxicity
Nanomaterials
Quantum dots