Ostkpr1 functions in anther cuticle development and pollen wall formation in rice

Xu, Dawei; Qu, Shuying; Tucker, Matthew R.; Zhang, Dabing; Liang, Wanqi; Shi, Jianxin

doi:10.1186/s12870-019-1711-4

Title: Ostkpr1 functions in anther cuticle development and pollen wall formation in rice

Journal Article · Mon Mar 18 00:00:00 EDT 2019 · BMC Plant Biology

DOI:https://doi.org/10.1186/s12870-019-1711-4· OSTI ID:1626851

Xu, Dawei ^[1]; Qu, Shuying ^[2]; Tucker, Matthew R. ^[3]; Zhang, Dabing ^[4]; Liang, Wanqi ^[2];

^[2]

Shanghai Jiao Tong Univ. (China). School of Life Sciences and Biotechnology. Shanghai Jiao Tong Univ.-Univ. of Adelaide Joint Centre for Agriculture and Health. Joint International Research Lab. of Metabolic & Developmental Sciences; Shanghai Jiao Tong Univ. (China). School of Life Sciences and Biotechnology. Flow Station of Post-doctoral Scientific Research
Shanghai Jiao Tong Univ. (China). School of Life Sciences and Biotechnology. Shanghai Jiao Tong Univ.-Univ. of Adelaide Joint Centre for Agriculture and Health. Joint International Research Lab. of Metabolic & Developmental Sciences
Univ. of Adelaide, SA (Australia). Food and Wine. School of Agriculture
Shanghai Jiao Tong Univ. (China). School of Life Sciences and Biotechnology. Shanghai Jiao Tong Univ.-Univ. of Adelaide Joint Centre for Agriculture and Health. Joint International Research Lab. of Metabolic & Developmental Sciences; Univ. of Adelaide, SA (Australia). Food and Wine. School of Agriculture

Background: During pollen wall formation in flowering plants, a conserved metabolon consisting of acyl-CoA synthetase (ACOS), polyketide synthase (PKS) and tetraketide α-pyrone reductase (TKPR), is required for sporopollenin synthesis. Despite this, the precise function of each of these components in different species remains unclear. Results: In this study, we characterized the function of OsTKPR1, a rice orthologue of Arabidopsis TKPR1. Loss of function of OsTKPR1 delayed tapetum degradation, reduced the levels of anther cuticular lipids, and impaired Ubisch body and pollen exine formation, resulting in complete male sterility. In addition, the phenylpropanoid pathway in mutant anthers was remarkably altered. Localization studies suggest that OsTKPR1 accumulates in the endoplasmic reticulum, while specific accumulation of OsTKPR1 mRNA in the anther tapetum and microspores is consistent with its function in anther and pollen wall development. Conclusions: Our results show that OsTKPR1 is indispensable for anther cuticle development and pollen wall formation in rice, providing new insights into the biochemical mechanisms of the conserved sporopollenin metabolon in flowering plants.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1626851

Journal Information:: BMC Plant Biology, Vol. 19, Issue 1; ISSN 1471-2229

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Plant Sciences
Anther cuticle
Oryza sativa
OsTKPR1
Pollen exine
Sporopollenin

Additional file 1: of Ostkpr1 functions in anther cuticle development and pollen wall formation in rice Xu, Dawei; Qu, Shuying; Tucker, Matthew https://doi.org/10.6084/m9.figshare.7861523 The current record is supplemented by this text	text	January 2019
Additional file 1: of Ostkpr1 functions in anther cuticle development and pollen wall formation in rice Xu, Dawei; Qu, Shuying; Tucker, Matthew https://doi.org/10.6084/m9.figshare.7861523.v1 The current record is supplemented by this text	text	January 2019
Additional file 2: of Ostkpr1 functions in anther cuticle development and pollen wall formation in rice Xu, Dawei; Qu, Shuying; Tucker, Matthew https://doi.org/10.6084/m9.figshare.7861529 The current record is supplemented by this image	image	January 2019
Additional file 2: of Ostkpr1 functions in anther cuticle development and pollen wall formation in rice Xu, Dawei; Qu, Shuying; Tucker, Matthew https://doi.org/10.6084/m9.figshare.7861529.v1 The current record is supplemented by this image	image	January 2019
Additional file 3: of Ostkpr1 functions in anther cuticle development and pollen wall formation in rice Xu, Dawei; Qu, Shuying; Tucker, Matthew https://doi.org/10.6084/m9.figshare.7861535 The current record is supplemented by this image	image	January 2019
Additional file 3: of Ostkpr1 functions in anther cuticle development and pollen wall formation in rice Xu, Dawei; Qu, Shuying; Tucker, Matthew https://doi.org/10.6084/m9.figshare.7861535.v1 The current record is supplemented by this image	image	January 2019
Additional file 4: of Ostkpr1 functions in anther cuticle development and pollen wall formation in rice Xu, Dawei; Qu, Shuying; Tucker, Matthew https://doi.org/10.6084/m9.figshare.7861541 The current record is supplemented by this text	text	January 2019
Additional file 4: of Ostkpr1 functions in anther cuticle development and pollen wall formation in rice Xu, Dawei; Qu, Shuying; Tucker, Matthew https://doi.org/10.6084/m9.figshare.7861541.v1 The current record is supplemented by this text	text	January 2019
Additional file 5: of Ostkpr1 functions in anther cuticle development and pollen wall formation in rice Xu, Dawei; Qu, Shuying; Tucker, Matthew https://doi.org/10.6084/m9.figshare.7861544 The current record is supplemented by this text	text	January 2019
Additional file 5: of Ostkpr1 functions in anther cuticle development and pollen wall formation in rice Xu, Dawei; Qu, Shuying; Tucker, Matthew https://doi.org/10.6084/m9.figshare.7861544.v1 The current record is supplemented by this text	text	January 2019
Additional file 6: of Ostkpr1 functions in anther cuticle development and pollen wall formation in rice Xu, Dawei; Qu, Shuying; Tucker, Matthew https://doi.org/10.6084/m9.figshare.7861547 The current record is supplemented by this text	text	January 2019
Additional file 6: of Ostkpr1 functions in anther cuticle development and pollen wall formation in rice Xu, Dawei; Qu, Shuying; Tucker, Matthew https://doi.org/10.6084/m9.figshare.7861547.v1 The current record is supplemented by this text	text	January 2019
Additional file 7: of Ostkpr1 functions in anther cuticle development and pollen wall formation in rice Xu, Dawei; Qu, Shuying; Tucker, Matthew https://doi.org/10.6084/m9.figshare.7861556 The current record is supplemented by this text	text	January 2019
Additional file 7: of Ostkpr1 functions in anther cuticle development and pollen wall formation in rice Xu, Dawei; Qu, Shuying; Tucker, Matthew https://doi.org/10.6084/m9.figshare.7861556.v1 The current record is supplemented by this text	text	January 2019
Additional file 8: of Ostkpr1 functions in anther cuticle development and pollen wall formation in rice Xu, Dawei; Qu, Shuying; Tucker, Matthew https://doi.org/10.6084/m9.figshare.7861559 The current record is supplemented by this image	image	January 2019
Additional file 8: of Ostkpr1 functions in anther cuticle development and pollen wall formation in rice Xu, Dawei; Qu, Shuying; Tucker, Matthew https://doi.org/10.6084/m9.figshare.7861559.v1 The current record is supplemented by this image	image	January 2019
Ostkpr1 functions in anther cuticle development and pollen wall formation in rice Xu, Dawei; Qu, Shuying; Tucker, Matthew https://doi.org/10.6084/m9.figshare.c.4438628 The current record is supplemented by this collection	collection	January 2019
Ostkpr1 functions in anther cuticle development and pollen wall formation in rice Xu, Dawei; Qu, Shuying; Tucker, Matthew https://doi.org/10.6084/m9.figshare.c.4438628.v1 The current record is supplemented by this collection	collection	January 2019