Three-dimensional morphology and gene expression in the Drosophila blastoderm at cellular resolution II: dynamics

Keranen, Soile V. E.; Fowlkes, Charless C.; Luengo Hendriks, Cris L.; Sudar, Damir; Knowles, David W.; Malik, Jitendra; Biggin, Mark D.

doi:10.1186/gb-2006-7-12-r124

**Three-dimensional morphology and gene expression in the Drosophila blastoderm at cellular resolution II: dynamics**

Journal Article · Wed Dec 20 23:00:00 EST 2006 · GenomeBiology.com

DOI:https://doi.org/10.1186/gb-2006-7-12-r124· OSTI ID:1626709

Keranen, Soile V. E. ^[1]; Fowlkes, Charless C. ^[2]; Luengo Hendriks, Cris L. ^[3]; Sudar, Damir ^[3]; Knowles, David W. ^[3]; Malik, Jitendra ^[2]; Biggin, Mark D. ^[3]

Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); DOE/OSTI
University of California, Berkeley, CA (United States)
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

To accurately describe gene expression and computationally model animal transcriptional networks, it is essential to determine the changing locations of cells in developing embryos. Using automated image analysis methods, we provide the first quantitative description of temporal changes in morphology and gene expression at cellular resolution in whole embryos, using the Drosophila blastoderm as a model. Analyses based on both fixed and live embryos reveal complex, previously undetected three-dimensional changes in nuclear density patterns caused by nuclear movements prior to gastrulation. Gene expression patterns move, in part, with these changes in morphology, but additional spatial shifts in expression patterns are also seen, supporting a previously proposed model of pattern dynamics based on the induction and inhibition of gene expression. We show that mutations that disrupt either the anterior/posterior (a/p) or the dorsal/ ventral (d/v) transcriptional cascades alter morphology and gene expression along both the a/p and d/v axes in a way suggesting that these two patterning systems interact via both transcriptional and morphological mechanisms. Our work establishes a new strategy for measuring temporal changes in the locations of cells and gene expression patterns that uses fixed cell material and computational modeling. It also provides a coordinate framework for the blastoderm embryo that will allow increasingly accurate spatio-temporal modeling of both the transcriptional control network and morphogenesis.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); Academy of Finland; National Institute of General Medical Sciences (NIGMS); National Human Genome Research Institute (NHGRI)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1626709

Journal Information:: GenomeBiology.com, Journal Name: GenomeBiology.com Journal Issue: 12 Vol. 7; ISSN 1465-6906

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

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**Three-dimensional morphology and gene expression in the Drosophila blastoderm at cellular resolution II: dynamics**