cpRAS: a novel circularly permuted RAS-like GTPase domain with a highly scattered phylogenetic distribution

Elias, Marek; Novotny, Marian

doi:10.1186/1745-6150-3-21

cpRAS: a novel circularly permuted RAS-like GTPase domain with a highly scattered phylogenetic distribution

Journal Article · Thu May 29 00:00:00 EDT 2008 · Biology Direct

DOI:https://doi.org/10.1186/1745-6150-3-21· OSTI ID:1626624

Elias, Marek ^[1]; Novotny, Marian ^[2]

Charles Univ., Prague (Czech Republic). Faculty of Science. Dept. of Botany; DOE/OSTI
Charles Univ., Prague (Czech Republic). Faculty of Science. Dept. of Cell Biology

A recent systematic survey suggested that the YRG (or YawG/YlqF) family with the G4-G5-G1- G2-G3 order of the conserved GTPase motifs represents the only possible circularly permuted variation of the canonical GTPase structure. Here we show that a different circularly permuted GTPase domain actually does exist, conforming to the pattern G3-G4-G5-G1-G2. The domain, dubbed cpRAS, is a variant of RAS family GTPases and occurs in two types of larger proteins, either inserted into a region homologous to a bacterial group of proteins classified as COG2373 and potentially related to the alpha-2-macroglobulin family (so far a single protein in Dictyostelium) or in combination with a von Willebrand factor type A (VWA) domain. For the latter protein type, which was found in a few metazoans and several distantly related protists, existence in the common ancestor of opisthokonts, Amoebozoa and excavates followed by at least eight independent losses may be inferred. Our findings thus bring further evidence for the importance of parallel reduction of ancestral complexity in the eukaryotic evolution.

View Accepted Manuscript (DOE)

Research Organization:: USDOE Joint Genome Institute (JGI), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

OSTI ID:: 1626624

Journal Information:: Biology Direct, Journal Name: Biology Direct Journal Issue: 1 Vol. 3; ISSN 1745-6150

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
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cpRAS: a novel circularly permuted RAS-like GTPase domain with a highly scattered phylogenetic distribution

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References (20)

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