The crystal structure of the catalytic domain of a eukaryotic guanylate cyclase

Winger, Jonathan A.; Derbyshire, Emily R.; Lamers, Meindert H.; Marletta, Michael A.; Kuriyan, John

doi:10.1186/1472-6807-8-42

The crystal structure of the catalytic domain of a eukaryotic guanylate cyclase

Journal Article · Tue Oct 07 00:00:00 EDT 2008 · BMC Structural Biology (Online)

DOI:https://doi.org/10.1186/1472-6807-8-42· OSTI ID:1626558

Winger, Jonathan A. ^[1]; Derbyshire, Emily R. ^[2]; Lamers, Meindert H. ^[2]; Marletta, Michael A. ^[3]; Kuriyan, John ^[4]

Univ. of California, Berkeley, CA (United States). Dept. of Molecular and Cell Biology; DOE/OSTI
Univ. of California, Berkeley, CA (United States). Dept. of Molecular and Cell Biology
Univ. of California, Berkeley, CA (United States). Dept. of Molecular and Cell Biology; Univ. of California, Berkeley, CA (United States). Dept. of Chemistry; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Division of Physical Biosciences
Univ. of California, Berkeley, CA (United States). Dept. of Molecular and Cell Biology; Univ. of California, Berkeley, CA (United States). Dept. of Chemistry; Univ. of California, Berkeley, CA (United States). Howard Hughes Medical Inst.

Background: Soluble guanylate cyclases generate cyclic GMP when bound to nitric oxide, thereby linking nitric oxide levels to the control of processes such as vascular homeostasis and neurotransmission. The guanylate cyclase catalytic module, for which no structure has been determined at present, is a class III nucleotide cyclase domain that is also found in mammalian membrane-bound guanylate and adenylate cyclases. Results: We have determined the crystal structure of the catalytic domain of a soluble guanylate cyclase from the green algae Chlamydomonas reinhardtii at 2.55 Å resolution, and show that it is a dimeric molecule. Conclusion: Comparison of the structure of the guanylate cyclase domain with the known structures of adenylate cyclases confirms the close similarity in architecture between these two enzymes, as expected from their sequence similarity. The comparison also suggests that the crystallized guanylate cyclase is in an inactive conformation, and the structure provides indications as to how activation might occur. We demonstrate that the two active sites in the dimer exhibit positive cooperativity, with a Hill coefficient of ~1.5. Positive cooperativity has also been observed in the homodimeric mammalian membrane-bound guanylate cyclases. The structure described here provides a reliable model for functional analysis of mammalian guanylate cyclases, which are closely related in sequence.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). National Energy Research Scientific Computing Center (NERSC)

Sponsoring Organization:: National Institutes of Health (NIH); USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

Grant/Contract Number:: AC02-05CH11231; AC03-76SF00098

OSTI ID:: 1626558

Journal Information:: BMC Structural Biology (Online), Journal Name: BMC Structural Biology (Online) Journal Issue: 1 Vol. 8; ISSN 1472-6807

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

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Molecular basis for GTP recognition by light‐activated guanylate cyclase Rh GC Butryn, Agata; Raza, Hadeeqa; Rada, Heather The FEBS Journal, Vol. 287, Issue 13 https://doi.org/10.1111/febs.15167	journal	December 2019
The brassinosteroid receptor BRI 1 can generate cGMP enabling cGMP ‐dependent downstream signaling Wheeler, Janet I.; Wong, Aloysius; Marondedze, Claudius The Plant Journal, Vol. 91, Issue 4 https://doi.org/10.1111/tpj.13589	journal	June 2017
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The Molecular Pharmacology of G Protein Signaling Then and Now: A Tribute to Alfred G. Gilman Sunahara, Roger K.; Insel, Paul A. Molecular Pharmacology, Vol. 89, Issue 5 https://doi.org/10.1124/mol.116.104216	journal	March 2016
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