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Genome-wide analysis of the PreA/PreB (QseB/QseC) regulon of Salmonella enterica serovar Typhimurium

Journal Article · · BMC Microbiology
 [1];  [2];  [3];  [4];  [5];  [5];  [6]
  1. The Ohio State Univ., Columbus, OH (United States). Center for Microbial Interface Biology. Dept. of Molecular Virology; Sandia National Lab. (SNL-NM), Albuquerque, NM (United States). Dept. of Biomolecular Materials and Interfaces; DOE/OSTI
  2. The Ohio State Univ., Columbus, OH (United States). Center for Microbial Interface Biology. Dept. of Molecular Virology; Univ. of Georgia, Athens, GA (United States). Dept. of Microbiology
  3. Univ. of Texas Health Science Center at San Antonio,TX (United States)
  4. The Ohio State Univ., Columbus, OH (United States). Center for Microbial Interface Biology. Dept. of Molecular Virology
  5. Sidney Kimmel Cancer Center, La Jolla, CA (United States)
  6. The Ohio State Univ., Columbus, OH (United States). Center for Microbial Interface Biology. Dept. of Molecular Virology; 1006 BRT, Columbus, OH (United States)
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Background: The Salmonella PreA/PreB two-component system (TCS) is an ortholog of the QseBC TCS of Escherichia coli. In both Salmonella and E. coli, this system has been shown to affect motility and virulence in response to quorum-sensing and hormonal signals, and to affect the transcription of the Salmonella enterica serovar Typhimurium (S. Typhimurium) pmrAB operon, which encodes an important virulence-associated TCS. Results: To determine the PreA/PreB regulon in S. Typhimurium, we performed DNA microarrays comparing the wild type strain and various preA and/or preB mutants in the presence of ectopically expressed preA (qseB). These data confirmed our previous findings of the negative effect of PreB on PreA gene regulation and identified candidate PreA-regulated genes. A proportion of the activated loci were previously identified as PmrA-activated genes (yibD, pmrAB, cptA, etc.) or were genes located in the local region around preA, including the preAB operon. The transcriptional units were defined in this local region by RT-PCR, suggesting three PreA activated operons composed of preA-preB, mdaB-ygiN, and ygiW-STM3175. Several putative virulence-related phenotypes were examined for preAB mutants, resulting in the observation of a host cell invasion and slight virulence defect of a preAB mutant. Contrary to previous reports on this TCS, we were unable to show a PreA/PreB-dependent effect of the quorum-sensing signal AI-2 or of epinephrine on S. Typhimurium with regard to bacterial motility. Conclusion: This work further characterizes this unorthadox OmpR/EnvZ class TCS and provides novel candidate regulated genes for further study. This first in-depth study of the PreA/PreB regulatory system phenotypes and regulation suggests significant comparative differences to the reported function of the orthologous QseB/QseC in E. coli.
Research Organization:
Sandia National Laboratories (SNL-NM), Albuquerque, NM (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division; National Institutes of Health (NIH)
Grant/Contract Number:
NA0003525
OSTI ID:
1626495
Journal Information:
BMC Microbiology, Journal Name: BMC Microbiology Journal Issue: 1 Vol. 9; ISSN 1471-2180
Publisher:
BioMed CentralCopyright Statement
Country of Publication:
United States
Language:
English

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Cited By (15)

Cinnamaldehyde, Carvacrol and Organic Acids Affect Gene Expression of Selected Oxidative Stress and Inflammation Markers in IPEC‐J2 Cells Exposed to Salmonella typhimurium journal August 2016
Aggregatibacter actinomycetemcomitans Qse BC is activated by catecholamines and iron and regulates genes encoding proteins associated with anaerobic respiration and metabolism journal June 2015
Qse BC , a two‐component bacterial adrenergic receptor and global regulator of virulence in Enterobacteriaceae and Pasteurellaceae journal November 2015
Signaling by two-component system noncognate partners promotes intrinsic tolerance to polymyxin B in uropathogenic Escherichia coli journal January 2017
A putative Vibrio cholerae two-component system controls a conserved periplasmic protein in response to the antimicrobial peptide polymyxin B journal October 2017
Gram-Negative Bacterial Sensors for Eukaryotic Signal Molecules journal September 2009
Molecular insights into farm animal and zoonotic Salmonella infections journal September 2009
Adrenergic antagonists restrict replication of Legionella journal July 2015
ygiW and qseBC are co-expressed in Aggregatibacter actinomycetemcomitans and regulate biofilm growth journal June 2013
New roles for two-component system response regulators of Salmonella enterica serovar Typhi during host cell interactions posted_content January 2019
A central metabolic circuit controlled by QseC in pathogenic Escherichia coli: Attenuation of qseC mutants is due to metabolic defects journal May 2011
Pathogen espionage: multiple bacterial adrenergic sensors eavesdrop on host communication systems: Pathogen-host communication journal December 2012
Selection of Salmonella enterica Serovar Typhi Genes Involved during Interaction with Human Macrophages by Screening of a Transposon Mutant Library journal May 2012
New Roles for Two-Component System Response Regulators of Salmonella enterica Serovar Typhi during Host Cell Interactions journal May 2020
Interkingdom crosstalk: Host neuroendocrine stress hormones drive the hemolytic behavior of Salmonella typhi journal July 2011

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