The Influence of Repressor DNA Binding Site Architecture on Transcriptional Control

Park, Dan M.; Kiley, Patricia J.

doi:10.1128/mbio.01684-14

The Influence of Repressor DNA Binding Site Architecture on Transcriptional Control

Journal Article · Tue Aug 26 00:00:00 EDT 2014 · mBio (Online)

DOI:https://doi.org/10.1128/mbio.01684-14· OSTI ID:1626136

Park, Dan M. ^[1]; Kiley, Patricia J. ^[2]

Univ. of Wisconsin, Madison, WI (United States). Dept. of Biomolecular Chemistry; DOE/OSTI
Univ. of Wisconsin, Madison, WI (United States). Dept. of Biomolecular Chemistry

How the architecture of DNA binding sites dictates the extent of repression of promoters is not well understood. Here, we addressed the importance of the number and information content of the three direct repeats (DRs) in the binding and repression of the icdA promoter by the phosphorylated form of the global Escherichia coli repressor ArcA (ArcA-P). We show that decreasing the information content of the two sites with the highest information (DR1 and DR2) eliminated ArcA binding to all three DRs and ArcA repression of icdA. Unexpectedly, we also found that DR3 occupancy functions principally in repression, since mutation of this low-information-content site both eliminated DNA binding to DR3 and significantly weakened icdA repression, despite the fact that binding to DR1 and DR2 was intact. In addition, increasing the information content of any one of the three DRs or addition of a fourth DR increased ArcA-dependent repression but perturbed signal-dependent regulation of repression. Thus, our data show that the information content and number of DR elements are critical architectural features for maintaining a balance between high-affinity binding and signal-dependent regulation of icdA promoter function in response to changes in ArcA-P levels. Optimization of such architectural features may be a common strategy to either dampen or enhance the sensitivity of DNA binding among the members of the large OmpR/PhoB family of regulators as well as other transcription factors.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of Wisconsin, Madison, WI (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

Grant/Contract Number:: FG02-04ER25627

OSTI ID:: 1626136

Journal Information:: mBio (Online), Journal Name: mBio (Online) Journal Issue: 5 Vol. 5; ISSN 2150-7511

Publisher:: American Society for Microbiology (ASM)Copyright Statement

Country of Publication:: United States

Language:: English

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