Function of Succinoglycan Polysaccharide in Sinorhizobium meliloti Host Plant Invasion Depends on Succinylation, Not Molecular Weight

Mendis, Hajeewaka C.; Madzima, Thelma F.; Queiroux, Clothilde; Jones, Kathryn M.

doi:10.1128/mbio.00606-16

Function of Succinoglycan Polysaccharide in Sinorhizobium meliloti Host Plant Invasion Depends on Succinylation, Not Molecular Weight

Journal Article · Tue Jun 21 00:00:00 EDT 2016 · mBio (Online)

DOI:https://doi.org/10.1128/mbio.00606-16· OSTI ID:1626114

Mendis, Hajeewaka C. ^[1]; Madzima, Thelma F. ^[2]; Queiroux, Clothilde ^[2]; Jones, Kathryn M. ^[2]

Florida State Univ., Tallahassee, FL (United States). Dept. of Biological Science; DOE/OSTI
Florida State Univ., Tallahassee, FL (United States). Dept. of Biological Science

The acidic polysaccharide succinoglycan produced by the rhizobial symbiont Sinorhizobium meliloti 1021 is required for this bacterium to invade the host plant Medicago truncatula and establish a nitrogen-fixing symbiosis. S. meliloti mutants that cannot make succinoglycan cannot initiate invasion structures called infection threads in plant root hairs. S. meliloti exoH mutants that cannot succinylate succinoglycan are also unable to form infection threads, despite the fact that they make large quantities of succinoglycan. Succinoglycan produced by exoH mutants is refractory to cleavage by the glycanases encoded by exoK and exsH, and thus succinoglycan produced by exoH mutants is made only in the high-molecular-weight (HMW) form. One interpretation of the symbiotic defect of exoH mutants is that the low-molecular-weight (LMW) form of succinoglycan is required for infection thread formation. However, our data demonstrate that production of the HMW form of succinoglycan by S. meliloti 1021 is sufficient for invasion of the host M. truncatula and that the LMW form is not required. Here, we show that S. meliloti strains deficient in the exoK- and exsH-encoded glycanases invade M. truncatula and form a productive symbiosis, although they do this with somewhat less efficiency than the wild type. We have also characterized the polysaccharides produced by these double glycanase mutants and determined that they consist of only HMW succinoglycan and no detectable LMW succinoglycan. This demonstrates that LMW succinoglycan is not required for host invasion. These results suggest succinoglycan function is not dependent upon the presence of a small, readily diffusible form.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of Georgia, Athens, GA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division; USDA

Grant/Contract Number:: FG02-93ER20097

OSTI ID:: 1626114

Journal Information:: mBio (Online), Journal Name: mBio (Online) Journal Issue: 3 Vol. 7; ISSN 2150-7511

Publisher:: American Society for Microbiology (ASM)Copyright Statement

Country of Publication:: United States

Language:: English

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Suppression of the Fix- phenotype of Rhizobium meliloti exoB mutants by lpsZ is correlated to a modified expression of the K polysaccharide. Reuhs, B. L.; Williams, M. N.; Kim, J. S. Journal of bacteriology, Vol. 177, Issue 15 https://doi.org/10.1128/jb.177.15.4289-4296.1995	journal	January 1995
Single-plant, Sterile Microcosms for Nodulation and Growth of the Legume Plant Medicago truncatula with the Rhizobial Symbiont Sinorhizobium meliloti Jones, Kathryn M.; Mendis, Hajeewaka C.; Queiroux, Clothilde Journal of Visualized Experiments, Issue 80 https://doi.org/10.3791/50916	journal	January 2013

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Surface Properties of Wild-Type Rhizobium leguminosarum bv. trifolii Strain 24.2 and Its Derivatives with Different Extracellular Polysaccharide Content Cieśla, Jolanta; Kopycińska, Magdalena; Łukowska, Małgorzata PLOS ONE, Vol. 11, Issue 10 https://doi.org/10.1371/journal.pone.0165080	journal	October 2016
Synthesis of Rhizobial Exopolysaccharides and Their Importance for Symbiosis with Legume Plants Marczak, Małgorzata; Mazur, Andrzej; Koper, Piotr Genes, Vol. 8, Issue 12 https://doi.org/10.3390/genes8120360	journal	December 2017
Sinorhizobium meliloti succinylated high‐molecular‐weight succinoglycan and the Medicago truncatula LysM receptor‐like kinase MtLYK10 participate independently in symbiotic infection Maillet, Fabienne; Fournier, Joëlle; Mendis, Hajeewaka C. The Plant Journal, Vol. 102, Issue 2 https://doi.org/10.1111/tpj.14625	journal	January 2020
Reports from a Healthy Community: the 7th Conference on Beneficial Microbes Mandel, Mark J.; Broderick, Nichole A.; Martens, Eric C. Applied and Environmental Microbiology, Vol. 85, Issue 10 https://doi.org/10.1128/aem.02562-18	journal	December 2018
Important Late-Stage Symbiotic Role of the Sinorhizobium meliloti Exopolysaccharide Succinoglycan Arnold, Markus F. F.; Penterman, Jon; Shabab, Mohammed Journal of Bacteriology, Vol. 200, Issue 13 https://doi.org/10.1128/jb.00665-17	journal	April 2018

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