Aromatic Amino Acids Required for Pili Conductivity and Long-Range Extracellular Electron Transport in Geobacter sulfurreducens

Vargas, Madeline; Malvankar, Nikhil S.; Tremblay, Pier-Luc; Leang, Ching; Smith, Jessica A.; Patel, Pranav; Synoeyenbos-West, Oona; Nevin, Kelly P.; Lovley, Derek R.

doi:10.1128/mbio.00105-13

Aromatic Amino Acids Required for Pili Conductivity and Long-Range Extracellular Electron Transport in Geobacter sulfurreducens

Journal Article · Tue Mar 12 00:00:00 EDT 2013 · mBio (Online)

DOI:https://doi.org/10.1128/mbio.00105-13· OSTI ID:1626085

Vargas, Madeline ^[1]; Malvankar, Nikhil S. ^[2]; Tremblay, Pier-Luc ^[2]; Leang, Ching ^[2]; Smith, Jessica A. ^[2]; Patel, Pranav ^[2]; Synoeyenbos-West, Oona ^[2]; Nevin, Kelly P. ^[2]; Lovley, Derek R. ^[2]

Univ. of Massachusetts, Amherst, MA (United States). Dept. of Microbiology; College of the Holy Cross, Worcester, MA (United States). Dept. of Biology; DOE/OSTI
Univ. of Massachusetts, Amherst, MA (United States). Dept. of Microbiology

It has been proposed that Geobacter sulfurreducens requires conductive pili for long-range electron transport to Fe(III) oxides and for high-density current production in microbial fuel cells. In order to investigate this further, we constructed a strain of G. sulfurreducens, designated Aro-5, which produced pili with diminished conductivity. This was accomplished by modifying the amino acid sequence of PilA, the structural pilin protein. An alanine was substituted for each of the five aromatic amino acids in the carboxyl terminus of PilA, the region in which G. sulfurreducens PilA differs most significantly from the PilAs of microorganisms incapable of long-range extracellular electron transport. Strain Aro-5 produced pili that were properly decorated with the multiheme c-type cytochrome OmcS, which is essential for Fe(III) oxide reduction. However, pili preparations of the Aro-5 strain had greatly diminished conductivity and Aro-5 cultures were severely limited in their capacity to reduce Fe(III) compared to the control strain. Current production of the Aro-5 strain, with a graphite anode serving as the electron acceptor, was less than 10% of that of the control strain. The conductivity of the Aro-5 biofilms was 10-fold lower than the control strain’s. These results demonstrate that the pili of G. sulfurreducens must be conductive in order for the cells to be effective in extracellular long-range electron transport.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of Massachusetts, Amherst, MA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

Grant/Contract Number:: SC0004114

OSTI ID:: 1626085

Journal Information:: mBio (Online), Journal Name: mBio (Online) Journal Issue: 2 Vol. 4; ISSN 2150-7511

Publisher:: American Society for Microbiology (ASM)Copyright Statement

Country of Publication:: United States

Language:: English

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The Low Conductivity of Geobacter uraniireducens Pili Suggests a Diversity of Extracellular Electron Transfer Mechanisms in the Genus Geobacter Tan, Yang; Adhikari, Ramesh Y.; Malvankar, Nikhil S. Frontiers in Microbiology, Vol. 07 https://doi.org/10.3389/fmicb.2016.00980	journal	June 2016
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