Listeria monocytogenes in Stone Fruits Linked to a Multistate Outbreak: Enumeration of Cells and Whole-Genome Sequencing

Chen, Yi; Burall, Laurel S.; Luo, Yan; Timme, Ruth; Melka, David; Muruvanda, Tim; Payne, Justin; Wang, Charles; Kastanis, George; Maounounen-Laasri, Anna; De Jesus, Antonio J.; Curry, Phillip E.; Stones, Robert; K'Aluoch, Okumu; Liu, Eileen; Salter, Monique; Hammack, Thomas S.; Evans, Peter S.; Parish, Mickey; Allard, Marc W.; Datta, Atin; Strain, Errol A.; Brown, Eric W.

doi:10.1128/aem.01486-16

Title: Listeria monocytogenes in Stone Fruits Linked to a Multistate Outbreak: Enumeration of Cells and Whole-Genome Sequencing

Journal Article · Mon Nov 21 00:00:00 EST 2016 · Applied and Environmental Microbiology

DOI:https://doi.org/10.1128/aem.01486-16· OSTI ID:1626065

Chen, Yi ^[1]; Burall, Laurel S. ^[2]; Luo, Yan ^[1]; Timme, Ruth ^[1]; Melka, David ^[1]; Muruvanda, Tim ^[1]; Payne, Justin ^[1]; Wang, Charles ^[1]; Kastanis, George ^[1]; Maounounen-Laasri, Anna ^[1]; De Jesus, Antonio J. ^[1]; Curry, Phillip E. ^[1]; Stones, Robert ^[3]; K'Aluoch, Okumu ^[4]; Liu, Eileen ^[4]; Salter, Monique ^[1]; Hammack, Thomas S. ^[1]; Evans, Peter S. ^[1]; Parish, Mickey ^[1]; Allard, Marc W. ^[1] more »

U.S. Food and Drug Administration (FDA), College Park, MD (United States). Center for Food Safety and Applied Nutrition
U.S. Food and Drug Administration (FDA), Laurel, MD (United States). Center for Food Safety and Applied Nutrition
Newcastle Univ., Newcastle upon Tyne (United Kingdom)
U.S. Food and Drug Administration (FDA), Alameda, CA (United States). Office of Regulatory Affairs

In 2014, the identification of stone fruits contaminated with led to the subsequent identification of a multistate outbreak. Simultaneous detection and enumeration of L. monocytogenes were performed on 105 fruits, each weighing 127 to 145 g, collected from 7 contaminated lots. The results showed that 53.3% of the fruits yielded L. monocytogenes (lower limit of detection, 5 CFU/fruit), and the levels ranged from 5 to 2,850 CFU/fruit, with a geometric mean of 11.3 CFU/fruit (0.1 CFU/g of fruit). Two serotypes, IVb-v1 and 1/2b, were identified by a combination of PCR- and antiserum-based serotyping among isolates from fruits and their packing environment; certain fruits contained a mixture of both serotypes. Single nucleotide polymorphism (SNP)-based whole-genome sequencing (WGS) analysis clustered isolates from two case-patients with the serotype IVb-v1 isolates and distinguished outbreak-associated isolates from pulsed-field gel electrophoresis (PFGE)-matched, but epidemiologically unrelated, clinical isolates. The outbreak-associated isolates differed by up to 42 SNPs. All but one serotype 1/2b isolate formed another WGS cluster and differed by up to 17 SNPs. Fully closed genomes of isolates from the stone fruits were used as references to maximize the resolution and to increase our confidence in prophage analysis. Putative prophages were conserved among isolates of each WGS cluster. All serotype IVb-v1 isolates belonged to singleton sequence type 382 (ST382); all but one serotype 1/2b isolate belonged to clonal complex 5.

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Research Organization:: Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: SC0014664

OSTI ID:: 1626065

Journal Information:: Applied and Environmental Microbiology, Vol. 82, Issue 24; ISSN 0099-2240

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

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An outbreak of multiple genotypes of Listeria monocytogenes in New Zealand linked to contaminated ready‐to‐eat meats—a retrospective analysis using whole‐genome sequencing Rivas, L.; Dupont, P. ‐Y.; Wilson, M. Letters in Applied Microbiology, Vol. 69, Issue 6 https://doi.org/10.1111/lam.13227	journal	October 2019
An Assessment of Different Genomic Approaches for Inferring Phylogeny of Listeria monocytogenes Henri, Clémentine; Leekitcharoenphon, Pimlapas; Carleton, Heather A. Frontiers in Microbiology, Vol. 8 https://doi.org/10.3389/fmicb.2017.02351	journal	November 2017
Assessing the genome level diversity of Listeria monocytogenes from contaminated ice cream and environmental samples linked to a listeriosis outbreak in the United States Chen, Yi; Luo, Yan; Curry, Phillip PLOS ONE, Vol. 12, Issue 2 https://doi.org/10.1371/journal.pone.0171389	journal	February 2017
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Survival of a serotype 4b strain and a serotype 1/2a strain of Listeria monocytogenes, isolated from a stone fruit outbreak investigation, on whole stone fruit at 4 °C De Jesus, Antonio J.; Sheth, Ishani; Kwon, Hee Jin International Journal of Food Microbiology, Vol. 334 https://doi.org/10.1016/j.ijfoodmicro.2020.108801	journal	December 2020
Whole genome sequencing analyses of Listeria monocytogenes that persisted in a milkshake machine for a year and caused illnesses in Washington State Li, Zhen; Pérez-Osorio, Ailyn; Wang, Yu BMC Microbiology, Vol. 17, Issue 1 https://doi.org/10.1186/s12866-017-1043-1	journal	June 2017
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Genetic Diversity of Listeria monocytogenes Isolated From Three Commercial Tree Fruit Packinghouses and Evidence of Persistent and Transient Contamination Chen, Yi; Simonetti, Tobin; Peter, Kari Frontiers in Microbiology, Vol. 12 https://doi.org/10.3389/fmicb.2021.756688	journal	January 2022
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