Provitamin A biofortification of cassava enhances shelf life but reduces dry matter content of storage roots due to altered carbon partitioning into starch

Beyene, Getu; Solomon, Felix R.; Chauhan, Raj D.; Gaitán-Solis, Eliana; Narayanan, Narayanan; Gehan, Jackson; Siritunga, Dimuth; Stevens, Robyn L.; Jifon, John; Van Eck, Joyce; Linsler, Edward; Gehan, Malia; Ilyas, Muhammad; Fregene, Martin; Sayre, Richard T.; Anderson, Paul; Taylor, Nigel J.; Cahoon, Edgar B.

doi:10.1111/pbi.12862

Provitamin A biofortification of cassava enhances shelf life but reduces dry matter content of storage roots due to altered carbon partitioning into starch

Journal Article · Wed Dec 27 00:00:00 EST 2017 · Plant Biotechnology Journal

DOI:https://doi.org/10.1111/pbi.12862· OSTI ID:1625924

Beyene, Getu ^[1]; Solomon, Felix R. ^[2]; Chauhan, Raj D. ^[3]; Gaitán-Solis, Eliana ^[3]; Narayanan, Narayanan ^[3]; Gehan, Jackson ^[3]; Siritunga, Dimuth ^[4]; Stevens, Robyn L. ^[3]; Jifon, John ^[5]; Van Eck, Joyce ^[6]; Linsler, Edward ^[6]; Gehan, Malia ^[3]; Ilyas, Muhammad ^[3]; Fregene, Martin ^[3]; Sayre, Richard T. ^[7]; Anderson, Paul ^[3]; ^[3]; ^[8]

Donald Danforth Plant Science Center, St. Louis, MO (United States); DOE/OSTI
Donald Danforth Plant Science Center, St. Louis, MO (United States); Univ. of Nebraska, Lincoln, NE (United States). Center for Plant Science Innovation. Dept. of Biochemistry
Donald Danforth Plant Science Center, St. Louis, MO (United States)
Univ. of Puerto Rico, Mayaguez (Puerto Rico). Dept. of Biology
Texas A & M Univ., College Station, TX (United States). Texas A & M AgriLife Research. Dept. of Horticultural Sciences
Boyce Thompson Inst., Ithaca, NY (United States)
Los Alamos National Lab. (LANL), Los Alamos, NM (United States). New Mexico Consortium
Donald Danforth Plant Science Center, St. Louis, MO (United States); Univ. of Nebraska, Lincoln, NE (United States). Center for Plant Science Innovation. Dept. of Biochemistry

Storage roots of cassava (Manihot esculenta Crantz), a major subsistence crop of sub-Saharan Africa, are calorie rich but deficient in essential micronutrients, including provitamin A bcarotene. In this study, b-carotene concentrations in cassava storage roots were enhanced by coexpression of transgenes for deoxy-D-xylulose-5-phosphate synthase (DXS) and bacterial phytoene synthase (crtB), mediated by the patatin-type 1 promoter. Storage roots harvested from field-grown plants accumulated carotenoids to ≤50 lg/g DW, 15- to 20-fold increases relative to roots from nontransgenic plants. Approximately 85%–90% of these carotenoids accumulated as all-trans-b-carotene, the most nutritionally efficacious carotenoid. b-Caroteneaccumulating storage roots displayed delayed onset of postharvest physiological deterioration, a major constraint limiting utilization of cassava products. Large metabolite changes were detected in b-carotene-enhanced storage roots. Most significantly, an inverse correlation was observed between b-carotene and dry matter content, with reductions of 50%–60% of dry matter content in the highest carotenoid-accumulating storage roots of different cultivars. Further analysis confirmed a concomitant reduction in starch content and increased levels of total fatty acids, triacylglycerols, soluble sugars and abscisic acid. Potato engineered to co-express DXS and crtB displayed a similar correlation between b-carotene accumulation, reduced dry matter and starch content and elevated oil and soluble sugars in tubers. Transcriptome analyses revealed a reduced expression of genes involved in starch biosynthesis including ADP-glucose pyrophosphorylase genes in transgenic, carotene-accumulating cassava roots relative to nontransgenic roots. These findings highlight unintended metabolic consequences of provitamin A biofortification of starch-rich organs and point to strategies for redirecting metabolic flux to restore starch production.

View Accepted Manuscript (DOE)

Research Organization:: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

Grant/Contract Number:: AC52-06NA25396

OSTI ID:: 1625924

Journal Information:: Plant Biotechnology Journal, Journal Name: Plant Biotechnology Journal Journal Issue: 6 Vol. 16; ISSN 1467-7644

Publisher:: Society for Experimental Biology; Association of Applied BiologyCopyright Statement

Country of Publication:: United States

Language:: English

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54 ENVIRONMENTAL SCIENCES
59 BASIC BIOLOGICAL SCIENCES
Biotechnology & Applied Microbiology
Plant Sciences
b-carotene
cassava
dry matter
fatty acid
provitamin A
starch

Provitamin A biofortification of cassava enhances shelf life but reduces dry matter content of storage roots due to altered carbon partitioning into starch

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References (71)

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