From model to crop: functional characterization of SPL8 in M.&nbsp;truncatula led to genetic improvement of biomass yield and abiotic stress tolerance in alfalfa

Gou, Jiqing; Debnath, Smriti; Sun, Liang; Flanagan, Amy; Tang, Yuhong; Jiang, Qingzhen; Wen, Jiangqi; Wang, Zeng-Yu

doi:10.1111/pbi.12841

From model to crop: functional characterization of SPL8 in M. truncatula led to genetic improvement of biomass yield and abiotic stress tolerance in alfalfa

Journal Article · Thu Sep 21 00:00:00 EDT 2017 · Plant Biotechnology Journal

DOI:https://doi.org/10.1111/pbi.12841· OSTI ID:1625923

Gou, Jiqing ^[1]; Debnath, Smriti ^[2]; Sun, Liang ^[2]; Flanagan, Amy ^[3]; Tang, Yuhong ^[3]; Jiang, Qingzhen ^[2]; Wen, Jiangqi ^[2]; ^[3]

Noble Research Inst., Ardmore OK (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); DOE/OSTI
Noble Research Inst., Ardmore OK (United States)
Noble Research Inst., Ardmore OK (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)

Biomass yield, salt tolerance and drought tolerance are important targets for alfalfa (Medicago sativa L.) improvement. Medicago truncatula has been developed into a model plant for alfalfa and other legumes. By screening a Tnt1 retrotransposon-tagged M. truncatula mutant population, we identified three mutants with enhanced branching. Branch development determines shoot architecture which affects important plant functions such as light acquisition, resource use and ultimately impacts biomass production. Molecular analyses revealed that the mutations were caused by Tnt1 insertions in the SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 8 (SPL8) gene. The M. truncatula spl8 mutants had increased biomass yield, while overexpression of SPL8 in M. truncatula suppressed branching and reduced biomass yield. Scanning electron microscopy (SEM) analysis showed that SPL8 inhibited branching by directly suppressing axillary bud formation. Based on the M. truncatula SPL8 sequence, alfalfa SPL8 (MsSPL8) was cloned and transgenic alfalfa plants were produced. MsSPL8 down-regulated or up-regulated alfalfa plants exhibited similar phenotypes to the M. truncatula mutants or overexpression lines, respectively. Specifically, the MsSPL8 down-regulated alfalfa plants showed up to 43% increase in biomass yield in the first harvest. The impact was even more prominent in the second harvest, with up to 86% increase in biomass production compared to the control. Furthermore, downregulation of MsSPL8 led to enhanced salt and drought tolerance in transgenic alfalfa. Results from this research offer a valuable approach to simultaneously improve biomass production and abiotic stress tolerance in legumes.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)

Sponsoring Organization:: Noble Research Institute, LLC; USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1625923

Journal Information:: Plant Biotechnology Journal, Journal Name: Plant Biotechnology Journal Journal Issue: 4 Vol. 16; ISSN 1467-7644

Publisher:: Society for Experimental Biology; Association of Applied BiologyCopyright Statement

Country of Publication:: United States

Language:: English

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Additional file 6 of Genome-wide identification, phylogeny and expression analysis of the SPL gene family and its important role in salt stress in Medicago sativa L. He, Fei; Long, Ruicai; Wei, Chunxue figshare https://doi.org/10.6084/m9.figshare.20079165.v1	image	January 2022
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From model to crop: functional characterization of SPL8 in M. truncatula led to genetic improvement of biomass yield and abiotic stress tolerance in alfalfa

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