Stabilization of an archaeal DNA-sliding clamp protein, PCNA, by proteasome-activating nucleotidase gene knockout in Haloferax volcanii

Kirkland, P. Aaron; Maupin-Furlow, Julie A.

doi:10.1111/j.1574-6968.2009.01547.x

Stabilization of an archaeal DNA-sliding clamp protein, PCNA, by proteasome-activating nucleotidase gene knockout in Haloferax volcanii

Journal Article · Fri May 01 00:00:00 EDT 2009 · FEMS Microbiology Letters

DOI:https://doi.org/10.1111/j.1574-6968.2009.01547.x· OSTI ID:1625903

Kirkland, P. Aaron ^[1]; Maupin-Furlow, Julie A. ^[2]

Univ. of Florida, Gainesville, FL (United States). Dept. of Microbiology and Cell Science; DOE/OSTI
Univ. of Florida, Gainesville, FL (United States). Dept. of Microbiology and Cell Science

Many details of structure, function and substrate specificity of eukaryotic proteasomal systems have been elucidated. This information far-exceeds that available for the archaeal and bacterial counterparts. While structural and functional studies have provided some insight into the workings of prokaryotic proteasomes, the question of substrate targeting and global cellular influence remain largely unaddressed. In this communication, we report an over 720-fold increase in the half-life of the DNA-sliding clamp protein proliferating cell nuclear antigen after knockout of the panA gene, encoding a proteasome-activating nucleotidase A, on the chromosome of the halophilic archaeon Haloferax volcanii. This discovery marks the first identification of a protein stabilized by an archaeal proteasome mutation and provides a starting point for investigations into substrate recognition mechanisms. The findings also begin to address the functional role of proteasomal systems within the scope of the archaeal cell.

Research Organization:: Univ. of Florida, Gainesville, FL (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: FG02-05ER15650

OSTI ID:: 1625903

Journal Information:: FEMS Microbiology Letters, Journal Name: FEMS Microbiology Letters Journal Issue: 1 Vol. 294; ISSN 0378-1097

Publisher:: Federation of European Microbiological SocietiesCopyright Statement

Country of Publication:: United States

Language:: English

References (13)

Degradation of proliferating cell nuclear antigen by 26S proteasome in rice ( Oryza sativa L.) Yamamoto, Taichi; Kimura, Seisuke; Mori, Yoko Planta, Vol. 218, Issue 4 https://doi.org/10.1007/s00425-003-1140-2	journal	February 2004
Mechanism of Gate Opening in the 20S Proteasome by the Proteasomal ATPases Rabl, Julius; Smith, David M.; Yu, Yadong Molecular Cell, Vol. 30, Issue 3 https://doi.org/10.1016/j.molcel.2008.03.004	journal	May 2008
Ubiquitin receptors and ERAD: A network of pathways to the proteasome Raasi, Shahri; Wolf, Dieter H. Seminars in Cell & Developmental Biology, Vol. 18, Issue 6 https://doi.org/10.1016/j.semcdb.2007.09.008	journal	December 2007
Ubiquitylation of Proliferating Cell Nuclear Antigen and Recruitment of Human DNA Polymerase η ^† Nikolaishvili-Feinberg, Nana; Jenkins, G. Scott; Nevis, Kathleen R. Biochemistry, Vol. 47, Issue 13 https://doi.org/10.1021/bi702329h	journal	April 2008
Genetic and Proteomic Analyses of a Proteasome-Activating Nucleotidase A Mutant of the Haloarchaeon Haloferax volcanii Kirkland, P. Aaron; Gil, Malgorzata A.; Karadzic, Ivanka M. Journal of Bacteriology, Vol. 190, Issue 1 https://doi.org/10.1128/jb.01196-07	journal	October 2007
Differential Regulation of the PanA and PanB Proteasome-Activating Nucleotidase and 20S Proteasomal Proteins of the Haloarchaeon Haloferax volcanii Reuter, Christopher J.; Kaczowka, Steven J.; Maupin-Furlow, Julie A. Journal of Bacteriology, Vol. 186, Issue 22 https://doi.org/10.1128/jb.186.22.7763-7772.2004	journal	November 2004
Transcriptome changes and cAMP oscillations in an archaeal cell cycle Baumann, Anke; Lange, Christian; Soppa, Jörg BMC Cell Biology, Vol. 8, Issue 1 https://doi.org/10.1186/1471-2121-8-21	journal	January 2007
Proteasomes from Structure to Function: Perspectives from Archaea Maupin‐Furlow, Julie A.; Humbard, Matthew A.; Kirkland, P. Aaron Current Topics in Developmental Biology https://doi.org/10.1016/S0070-2153(06)75005-0	book	January 2006
PCNA, the Maestro of the Replication Fork Moldovan, George-Lucian; Pfander, Boris; Jentsch, Stefan Cell, Vol. 129, Issue 4 https://doi.org/10.1016/j.cell.2007.05.003	journal	May 2007
Tyrosine phosphorylation controls PCNA function through protein stability Wang, Shao-Chun; Nakajima, Yusuke; Yu, Yung-Luen Nature Cell Biology, Vol. 8, Issue 12 https://doi.org/10.1038/ncb1501	journal	November 2006
A new simvastatin (mevinolin)-resistance marker from Haloarcula hispanica and a new Haloferax volcanii strain cured of plasmid pHV2 Dyall-Smith, M. L.; Wendoloski, D.; Ferrer, C. Microbiology, Vol. 147, Issue 4 https://doi.org/10.1099/00221287-147-4-959	journal	April 2001
Genetic and Proteomic Analyses of a Proteasome-Activating Nucleotidase A Mutant of the Haloarchaeon Haloferax volcanii Kirkland, P. Aaron; Gil, Malgorzata A.; Karadzic, Ivanka M. Journal of Bacteriology, Vol. 190, Issue 1 https://doi.org/10.1128/JB.01196-07	journal	October 2007
Differential Regulation of the PanA and PanB Proteasome-Activating Nucleotidase and 20S Proteasomal Proteins of the Haloarchaeon Haloferax volcanii Reuter, Christopher J.; Kaczowka, Steven J.; Maupin-Furlow, Julie A. Journal of Bacteriology, Vol. 186, Issue 22 https://doi.org/10.1128/JB.186.22.7763-7772.2004	journal	November 2004

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59 BASIC BIOLOGICAL SCIENCES
DNA polymerase
Microbiology
archaea
halophile
proteasome
proteolysis
sliding clamp

Stabilization of an archaeal DNA-sliding clamp protein, PCNA, by proteasome-activating nucleotidase gene knockout in Haloferax volcanii

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References (13)

Cited By (2)

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