Structure of aldose reductase from Giardia lamblia

Ferrell, M.; Abendroth, J.; Zhang, Y.; Sankaran, B.; Edwards, T. E.; Staker, B. L.; Van Voorhis, W. C.; Stewart, L. J.; Myler, P. J.

doi:10.1107/s1744309111030879

Structure of aldose reductase from Giardia lamblia

Journal Article · Tue Aug 16 00:00:00 EDT 2011 · Acta Crystallographica. Section F

DOI:https://doi.org/10.1107/s1744309111030879· OSTI ID:1625814

Ferrell, M. ^[1]; Abendroth, J. ^[2]; Zhang, Y. ^[3]; Sankaran, B. ^[4]; Edwards, T. E. ^[2]; Staker, B. L. ^[2]; Van Voorhis, W. C. ^[5]; Stewart, L. J. ^[2]; Myler, P. J. ^[3]

Seattle Structural Genomics Center for Infectious Disease, Seattle, WA (United States); Seattle Biomedical Research Inst., Seattle, WA (United States); DOE/OSTI
Seattle Structural Genomics Center for Infectious Disease, Seattle, WA (United States); Emerald BioStructures Inc., Bainbridge Island, WA (United States)
Seattle Structural Genomics Center for Infectious Disease, Seattle, WA (United States); Seattle Biomedical Research Inst., Seattle, WA (United States)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Berkeley Center For Structural Biology
Seattle Structural Genomics Center for Infectious Disease, Seattle, WA (United States); Univ. of Washington, Seattle, WA (United States). School of Medicine

Giardia lamblia is an anaerobic aerotolerant eukaryotic parasite of the intestines. It is believed to have diverged early from eukarya during evolution and is thus lacking in many of the typical eukaryotic organelles and biochemical pathways. Most conspicuously, mitochondria and the associated machinery of oxidative phosphorylation are absent; instead, energy is derived from substratelevel phosphorylation. Here, the 1.75 Å resolution crystal structure of G. lamblia aldose reductase heterologously expressed in Escherichia coli is reported. As in other oxidoreductases, G. lamblia aldose reductase adopts a TIM-barrel conformation with the NADP+ -binding site located within the eight -strands of the interior.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1625814

Journal Information:: Acta Crystallographica. Section F, Journal Name: Acta Crystallographica. Section F Journal Issue: 9 Vol. 67; ISSN ACSFCL; ISSN 1744-3091

Publisher:: International Union of CrystallographyCopyright Statement

Country of Publication:: United States

Language:: English

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