Structure of a tryptophanyl-tRNA synthetase containing an iron–sulfur cluster

Journal Article · Thu Sep 23 00:00:00 EDT 2010 · Acta Crystallographica. Section F

DOI:https://doi.org/10.1107/s1744309110037619· OSTI ID:1625806

Han, Gye Won ^[1]; Yang, Xiang-Lei ^[2]; McMullan, Daniel ^[3]; Chong, Yeeting E. ^[2]; Krishna, S. Sri ^[4]; Rife, Christopher L. ^[5]; Weekes, Dana ^[6]; Brittain, Scott M. ^[7]; Abdubek, Polat ^[3]; Ambing, Eileen ^[3]; Astakhova, Tamara ^[8]; Axelrod, Herbert L. ^[5]; Carlton, Dennis ^[2]; Caruthers, Jonathan ^[5]; Chiu, Hsiu-Ju ^[5]; Clayton, Thomas ^[2]; Duan, Lian ^[8]; Feuerhelm, Julie ^[3]; Grant, Joanna C. ^[3]; Grzechnik, Slawomir K. ^[8] ; Jaroszewski, Lukasz ^[4]; Jin, Kevin K. ^[5]; Klock, Heath E. ^[3]; Knuth, Mark W. ^[3]; Kumar, Abhinav ^[5]; Marciano, David ^[2]; Miller, Mitchell D. ^[5]; Morse, Andrew T. ^[8]; Nigoghossian, Edward ^[3]; Okach, Linda ^[3]; Paulsen, Jessica ^[3]; Reyes, Ron ^[5]; van den Bedem, Henry ^[5]; White, Aprilfawn ^[3]; Wolf, Guenter ^[5]; Xu, Qingping ^[5]; Hodgson, Keith O. ^[9]; Wooley, John ^[8]; Deacon, Ashley M. ^[5]; Godzik, Adam ^[4]; Lesley, Scott A. ^[3]; Elsliger, Marc-André ^[2]; Schimmel, Paul ^[2]; Wilson, Ian A. ^[2]

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1. The Scripps Research Inst., La Jolla, CA (United States); DOE/OSTI
2. The Scripps Research Inst., La Jolla, CA (United States)
3. The Scripps Research Inst., La Jolla, CA (United States); Genomics Inst. of the Novartis Research Foundation, San Diego, CA (United States)
4. The Scripps Research Inst., La Jolla, CA (United States); Univ. of California, San Diego, La Jolla, CA (United States); Sanford–Burnham Medical Research Inst., La Jolla, CA (United States)
5. The Scripps Research Inst., La Jolla, CA (United States); SLAC National Accelerator Lab., Menlo Park, CA (United States). Stanford Synchrotron Radiation Lightsource (SSRL)
6. The Scripps Research Inst., La Jolla, CA (United States); Sanford–Burnham Medical Research Inst., La Jolla, CA (United States)
7. Genomics Inst. of the Novartis Research Foundation, San Diego, CA (United States)
8. The Scripps Research Inst., La Jolla, CA (United States); Univ. of California, San Diego, La Jolla, CA (United States)
9. The Scripps Research Inst., La Jolla, CA (United States); SLAC National Accelerator Lab., Menlo Park, CA (United States)

A novel aminoacyl-tRNA synthetase that contains an iron–sulfur cluster in the tRNA anticodon-binding region and efficiently charges tRNA with tryptophan has been found in Thermotoga maritima. The crystal structure of TmTrpRS (tryptophanyl-tRNA synthetase; TrpRS; EC 6.1.1.2) reveals an iron–sulfur [4Fe–4S] cluster bound to the tRNA anticodon-binding (TAB) domain and an l-tryptophan ligand in the active site. None of the other T. maritima aminoacyltRNA synthetases (AARSs) contain this [4Fe–4S] cluster-binding motif (C-x22- C-x6-C-x2-C). It is speculated that the iron–sulfur cluster contributes to the stability of TmTrpRS and could play a role in the recognition of the anticodon.

• Research Organization: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States). Advanced Light Source (ALS)
• Sponsoring Organization: National Center for Research Resources (NCRR); National Institute of General Medical Sciences (NIGMS); National Institutes of Health (NIH); USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
• Grant/Contract Number: AC02-05CH11231
• OSTI ID: 1625806
• Journal Information: Acta Crystallographica. Section F, Journal Name: Acta Crystallographica. Section F Journal Issue: 10 Vol. 66; ISSN ACSFCL; ISSN 1744-3091
• Publisher: International Union of Crystallography
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
TM0492
biochemistry & molecular biology
biophysics
crystallography
iron–sulfur clusters
structural genomics.
tryptophanyl-tRNA ligase
tryptophanyl-tRNA synthetase class I