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The structure of the first representative of Pfam family PF06475 reveals a new fold with possible involvement in glycolipid metabolism

Journal Article · · Acta Crystallographica. Section F
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  1. Joint Center for Structural Genomics, http://www.jcsg.org (United States); Burnham Inst. for Medical Research, La Jolla, CA (United States). Program on Bioinformatics and Systems Biology; DOE/OSTI
  2. Joint Center for Structural Genomics, http://www.jcsg.org (United States); SLAC National Accelerator Lab., Menlo Park, CA (United States). Stanford Synchrotron Radiation Lightsource (SSRL)
  3. Joint Center for Structural Genomics, http://www.jcsg.org (United States); Genomics Institute of the Novartis Research Foundation, San Diego, CA (United States). Protein Sciences Dept.
  4. Joint Center for Structural Genomics, http://www.jcsg.org (United States); Burnham Inst. for Medical Research, La Jolla, CA (United States). Program on Bioinformatics and Systems Biology; Univ. of California, San Diego, La Jolla, CA (United States). Center for Research in Biological Systems
  5. Joint Center for Structural Genomics, http://www.jcsg.org (United States); The Scripps Research Inst., La Jolla, CA (United States). Dept. of Molecular Biology
  6. Universite de Sherbrooke, Quebec (Canada). Dept. de Biochimie
  7. Joint Center for Structural Genomics, http://www.jcsg.org (United States); Univ. of California, San Diego, La Jolla, CA (United States). Center for Research in Biological Systems
  8. Joint Center for Structural Genomics (United States). http://www.jcsg.org; Burnham Inst. for Medical Research, La Jolla, CA (United States). Program on Bioinformatics and Systems Biology; Univ. of California, San Diego, La Jolla, CA (United States). Center for Research in Biological Systems
  9. Joint Center for Structural Genomics, http://www.jcsg.org (United States); Burnham Inst. for Medical Research, La Jolla, CA (United States). Program on Bioinformatics and Systems Biology
  10. Joint Center for Structural Genomics, http://www.jcsg.org (United States; SLAC National Accelerator Lab., Menlo Park, CA (United States). Photon Science
  11. Joint Center for Structural Genomics, http://www.jcsg.org (United States); Genomics Institute of the Novartis Research Foundation, San Diego, CA (United States). Protein Sciences Dept.; The Scripps Research Inst., La Jolla, CA (United States). Dept. of Molecular Biology
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The crystal structure of PA1994 from Pseudomonas aeruginosa, a member of the Pfam PF06475 family classified as a domain of unknown function (DUF1089), reveals a novel fold comprising a 15-stranded -sheet wrapped around a single -helix that assembles into a tight dimeric arrangement. The remote structural similarity to lipoprotein localization factors, in addition to the presence of an acidic pocket that is conserved in DUF1089 homologs, phospholipid-binding and sugar-binding proteins, indicate a role for PA1994 and the DUF1089 family in glycolipid metabolism. Genome-context analysis lends further support to the involvement of this family of proteins in glycolipid metabolism and indicates possible activation of DUF1089 homologs under conditions of bacterial cell-wall stress or host–pathogen interactions.
Research Organization:
SLAC National Accelerator Laboratory, Menlo Park, CA (United States). Stanford Synchrotron Radiation Lightsource (SSRL)
Sponsoring Organization:
USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE Office of Science (SC), Biological and Environmental Research (BER)
Grant/Contract Number:
AC02-76SF00515
OSTI ID:
1625786
Journal Information:
Acta Crystallographica. Section F, Journal Name: Acta Crystallographica. Section F Journal Issue: 10 Vol. 66; ISSN ACSFCL; ISSN 1744-3091
Publisher:
International Union of CrystallographyCopyright Statement
Country of Publication:
United States
Language:
English

Cited By (4)

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Brucella Periplasmic Protein EipB Is a Molecular Determinant of Cell Envelope Integrity and Virulence journal April 2019
Large-scale detection of drug off-targets: hypotheses for drug repurposing and understanding side-effects journal April 2017
IsoCleft Finder – a web-based tool for the detection and analysis of protein binding-site geometric and chemical similarities journal January 2013

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DUFs
biochemistry & molecular biology
biophysics
crystallography
glycolipids
host–pathogen interactions
osmotic stress
structural genomics