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Title: Regulation and Levels of the Thylakoid K + /H + Antiporter KEA3 Shape the Dynamic Response of Photosynthesis in Fluctuating Light

Journal Article · · Plant and Cell Physiology
DOI:https://doi.org/10.1093/pcp/pcw085· OSTI ID:1625582
 [1];  [2];  [3];  [3];  [2];  [4];  [5]
  1. Univ. of California, Berkeley, CA (United States). Dept. of Plant and Microbial Biology. Howard Hughes Medical Inst.; Carnegie Inst. for Science, Stanford, CA (United States). Dept. of Plant Biology; Max Planck Inst. of Molecular Plant Physiology, Potsdam (Germany)
  2. Univ. of California, Berkeley, CA (United States). Dept. of Plant and Microbial Biology. Howard Hughes Medical Inst.
  3. Max Planck Inst. of Molecular Plant Physiology, Potsdam (Germany)
  4. Carnegie Inst. for Science, Stanford, CA (United States). Dept. of Plant Biology
  5. Univ. of California, Berkeley, CA (United States). Dept. of Plant and Microbial Biology. Howard Hughes Medical Inst.; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Physical Biosciences Division
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Crop canopies create environments of highly fluctuating light intensities. In such environments, photoprotective mechanisms and their relaxation kinetics have been hypothesized to limit photosynthetic efficiency and therefore crop yield potential. Here, we show that overexpression of the Arabidopsis thylakoid K+/H+ antiporter KEA3 accelerates the relaxation of photoprotective energy-dependent quenching after transitions from high to low light in Arabidopsis and tobacco. This, in turn, enhances PSII quantum efficiency in both organisms, supporting that in wild-type plants, residual light energy quenching following a high to low light transition represents a limitation to photosynthetic efficiency in fluctuating light. This finding underscores the potential of accelerating quenching relaxation as a building block for improving photosynthetic efficiency in the field. Additionally, by overexpressing natural KEA3 variants with modification to the C-terminus, we show that KEA3 activity is regulated by a mechanism involving its lumen-localized C-terminus, which lowers KEA3 activity in high light. This regulatory mechanism finetunes the balance between photoprotective energy dissipation in high light and maximum quantum yield in low light, likely to be critical for efficient photosynthesis in fluctuating light conditions.

Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
Grant/Contract Number:
AC02-05CH11231
OSTI ID:
1625582
Journal Information:
Plant and Cell Physiology, Vol. 57, Issue 7; ISSN 0032-0781
Publisher:
Japanese Society of Plant PhysiologistsCopyright Statement
Country of Publication:
United States
Language:
English

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Cited By (10)

Fluctuating light experiments and semi-automated plant phenotyping enabled by self-built growth racks and simple upgrades to the IMAGING-PAM journal December 2019
Maintenance of pre-existing DNA methylation states through recurring excess-light stress: A stable methylome through recurring light stress journal May 2018
Salt-Enhanced Reproductive Development of Suaeda salsa L. Coincided With Ion Transporter Gene Upregulation in Flowers and Increased Pollen K+ Content journal March 2019
K+ and Cl− channels/transporters independently fine-tune photosynthesis in plants journal June 2019
A New Light on Photosystem II Maintenance in Oxygenic Photosynthesis journal July 2019
Evidence for potassium transport activity of Arabidopsis KEA1-KEA6 journal July 2019
Less photoprotection can be good in some genetic and environmental contexts journal July 2019
Genetic characterization of a flap1 null mutation in Arabidopsis npq4 and pgr5 plants suggests that the regulatory role of FLAP1 involves the control of proton homeostasis in chloroplasts journal November 2018
Efficient photosynthesis in dynamic light environments: a chloroplast's perspective journal October 2019
Optimization of Photosynthetic Productivity in Contrasting Environments by Regulons Controlling Plant Form and Function journal March 2018

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Plant Sciences
Cell Biology
Arabidopsis
KEA3
Non-photochemical quenching
PSII quantum efficiency
Thylakoid membrane