The RRM of the kRNA-editing protein TbRGG2 uses multiple surfaces to bind and remodel RNA

Travis, Brady; Shaw, Porsha L. R.; Liu, Bei; Ravindra, Krishna; Iliff, Hadley; Al-Hashimi, Hashim M; Schumacher, Maria A.

doi:10.1093/nar/gky1259

Title: The RRM of the kRNA-editing protein TbRGG2 uses multiple surfaces to bind and remodel RNA

Journal Article · Fri Dec 14 00:00:00 EST 2018 · Nucleic Acids Research

DOI:https://doi.org/10.1093/nar/gky1259· OSTI ID:1625573

Travis, Brady ^[1]; Shaw, Porsha L. R. ^[1]; Liu, Bei ^[1]; Ravindra, Krishna ^[1]; Iliff, Hadley ^[1]; Al-Hashimi, Hashim M ^[1]; Schumacher, Maria A. ^[1]

Duke Univ., Durham, NC (United States)

Kinetoplastid RNA (kRNA) editing takes place in the mitochondria of kinetoplastid protists and creates translatable mRNAs by uridine insertion/deletion. Extensively edited (pan-edited) transcripts contain quadruplex forming guanine stretches, which must be remodeled to promote uridine insertion/deletion. Here we show that the RRM domain of the essential kRNA-editing factor TbRGG2 binds poly(G) and poly(U) RNA and can unfold both. A region C-terminal to the RRM mediates TbRGG2 dimerization, enhancing RNA binding. A RRM-U4 RNA structure reveals a unique RNA-binding mechanism in which the two RRMs of the dimer employ aromatic residues outside the canonical RRM RNA-binding motifs to encase and wrench open the RNA, while backbone atoms specify the uridine bases. Notably, poly(G) RNA is bound via a different binding surface. Thus, these data indicate that TbRGG2 RRM can bind and remodel several RNA substrates suggesting how it might play multiple roles in the kRNA editing process.

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Research Organization:: Duke Univ., Durham, NC (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Scientific User Facilities Division

Grant/Contract Number:: AC02-05CH11231; P01GM0066275

OSTI ID:: 1625573

Journal Information:: Nucleic Acids Research, Vol. 47, Issue 4; ISSN 0305-1048

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 5 works

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