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The Streptomyces master regulator BldD binds c-di-GMP sequentially to create a functional BldD2-(c-di-GMP)4 complex

Journal Article · · Nucleic Acids Research
DOI:https://doi.org/10.1093/nar/gkx287· OSTI ID:1625563
 [1];  [2];  [3];  [3];  [2];  [4]
  1. Duke Univ., Durham, NC (United States). School of Medicine. Dept. of Biochemistry; DOE/OSTI
  2. Duke Univ., Durham, NC (United States). School of Medicine. Dept. of Biochemistry
  3. John Innes Centre, Norwich (United Kingdom). Norwich Research Park. Dept. of Molecular Microbiology
  4. Humboldt Univ. of Berlin (Germany). Institut fur Biologie/Mikrobiologie
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Streptomyces are ubiquitous soil bacteria that undergo a complex developmental transition coinciding with their production of antibiotics. This transition is controlled by binding of a novel tetrameric form of the second messenger, 3 -5 cyclic diguanylic acid (c-diGMP) to the master repressor, BldD. In all domains of life, nucleotide-based second messengers allow a rapid integration of external and internal signals into regulatory pathways that control cellular responses to changing conditions. c-di-GMP can assume alternative oligomeric states to effect different functions, binding to effector proteins as monomers, intercalated dimers or, uniquely in the case of BldD, as a tetramer. However, at physiological concentrations c-di-GMP is a monomer and little is known about how higher oligomeric complexes assemble on effector proteins and if intermediates in assembly pathways have regulatory significance. Here, we show that c-di-GMP binds BldD using an ordered, sequential mechanism and that BldD function necessitates the assembly of the BldD2-(c-di-GMP)4 complex.
Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
Grant/Contract Number:
AC02-05CH11231
OSTI ID:
1625563
Journal Information:
Nucleic Acids Research, Journal Name: Nucleic Acids Research Journal Issue: 11 Vol. 45; ISSN 0305-1048
Publisher:
Oxford University PressCopyright Statement
Country of Publication:
United States
Language:
English

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Cited By (10)

The MerR-like protein BldC binds DNA direct repeats as cooperative multimers to regulate Streptomyces development journal March 2018
BrlR from Pseudomonas aeruginosa is a receptor for both cyclic di-GMP and pyocyanin journal July 2018
The crystal structure of the RsbN–σBldN complex from Streptomyces venezuelae defines a new structural class of anti-σ factor journal July 2018
Expression Patterns, Genomic Conservation and Input Into Developmental Regulation of the GGDEF/EAL/HD-GYP Domain Proteins in Streptomyces journal October 2018
Hypertension, Hyperreninemia, and Secondary Hyperaldosteronism in Systemic Necrotizing Vasculitis journal February 1980
Overexpression of the diguanylate cyclase CdgD blocks developmental transitions and antibiotic biosynthesis in Streptomyces coelicolor journal June 2019
VpsR and cyclic di-GMP together drive transcription initiation to activate biofilm formation in Vibrio cholerae journal July 2018
Nucleotide second messengers in Streptomyces journal November 2019
Regulation of sporangium formation by the orphan response regulator TcrA in the rare actinomycete Actinoplanes missouriensis: Regulation of sporangium formation in Actinoplanes journal February 2018
BldC Delays Entry into Development To Produce a Sustained Period of Vegetative Growth in Streptomyces venezuelae journal February 2019

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