One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome
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December 2008 |
Resolution of ColE1 dimers requires a DNA sequence implicated in the three-dimensional organization of the cer site.
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March 1988 |
Two-step site selection for serine-integrase-mediated excision: DNA-directed integrase conformation and central dinucleotide proofreading
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February 2008 |
Turbo cloning: a fast, efficient method for cloning PCR products and other blunt-ended DNA fragments into plasmids
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January 1993 |
In vitro site-specific integration of bacteriophage DNA catalyzed by a recombinase of the resolvase/invertase family
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May 1998 |
Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction Enzymes
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May 2009 |
Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC
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journal
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February 2007 |
Circular Polymerase Extension Cloning of Complex Gene Libraries and Pathways
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July 2009 |
Tandem assembly of the epothilone biosynthetic gene cluster by in vitro site-specific recombination
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November 2011 |
Evidence for high specificity and efficiency of multiple recombination signals in mixed DNA cloning by the Multisite Gateway system
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February 2004 |
Metabolic engineering of carotenoid accumulation in Escherichia coli by modulation of the isoprenoid precursor pool with expression of deoxyxylulose phosphate synthase
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April 2000 |
Engineering the lycopene synthetic pathway in E. coli by comparison of the carotenoid genes of Pantoea agglomerans and Pantoea ananatis
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November 2006 |
SLiCE: a novel bacterial cell extract-based DNA cloning method
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January 2012 |
Synthesis of DNA fragments in yeast by one-step assembly of overlapping oligonucleotides
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September 2009 |
DNA Cloning Using In Vitro Site-Specific Recombination
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November 2000 |
Enzymatic assembly of DNA molecules up to several hundred kilobases
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April 2009 |
A new family of mobilizable suicide plasmids based on broad host range R388 plasmid (IncW) and RP4 plasmid (IncPα) conjugative machineries and their cognate Escherichia coli host strains
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March 2005 |
Production of zeaxanthin in Escherichia coli transformed with different carotenogenic plasmids
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August 1997 |
Switching the polarity of a bacteriophage integration system: Polarity of phage integration
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February 2004 |
Concerted Assembly and Cloning of Multiple DNA Segments Using In Vitro Site-Specific Recombination: Functional Analysis of Multi-Segment Expression Clones
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October 2004 |
Identification of genes affecting lycopene accumulation inEscherichia coli using a shot-gun method
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journal
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January 2005 |
DNA assembler, an in vivo genetic method for rapid construction of biochemical pathways
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journal
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December 2008 |
Automated design of synthetic ribosome binding sites to control protein expression
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journal
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October 2009 |
Metabolic engineering of the nonmevalonate isopentenyl diphosphate synthesis pathway inEscherichia coli enhances lycopene production
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journal
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January 2001 |
A phage protein that binds φC31 integrase to switch its directionality: Excision by φC31 integrase
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May 2011 |
Cell killing by the F plasmid CcdB protein involves poisoning of DNA-topoisomerase II complexes
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August 1992 |
BglBricks: A flexible standard for biological part assembly
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January 2010 |
Gated rotation mechanism of site-specific recombination by C31 integrase
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November 2012 |
Engineering BioBrick vectors from BioBrick parts
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January 2008 |
Metabolic Engineering of Carotenoid Biosynthesis in Escherichia coli by Ordered Gene Assembly in Bacillus subtilis
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December 2006 |
Reevaluation of the Violacein Biosynthetic Pathway and its Relationship to Indolocarbazole Biosynthesis
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July 2006 |
Investigation of factors influencing production of the monocyclic carotenoid torulene in metabolically engineered Escherichia coli
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May 2004 |
A One Pot, One Step, Precision Cloning Method with High Throughput Capability
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November 2008 |
General Method for Plasmid Construction Using Homologous Recombination
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January 1999 |
Synapsis and DNA cleavage in C31 integrase-mediated site-specific recombination
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April 2004 |
Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements
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March 1997 |
Control of directionality in the site-specific recombination system of the Streptomyces phage phiC31
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journal
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October 2000 |
Expression of an exogenous isopentenyl diphosphate isomerase gene enhances isoprenoid biosynthesis in Escherichia coli
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June 1997 |
DNA assembly for synthetic biology: from parts to pathways and beyond
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January 2011 |
Site-specific recombination by φC31 integrase and other large serine recombinases
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March 2010 |
Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli.
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January 1990 |