Mitotic centromeric targeting of HP1 and its binding to Sgo1 are dispensable for sister-chromatid cohesion in human cells

Kang, Jungseog; Chaudhary, Jaideep; Dong, Hui; Kim, Soonjoung; Brautigam, Chad A.; Yu, Hongtao

doi:10.1091/mbc.e11-01-0009

Mitotic centromeric targeting of HP1 and its binding to Sgo1 are dispensable for sister-chromatid cohesion in human cells

Journal Article · Wed Feb 23 00:00:00 EST 2011 · Molecular Biology of the Cell

DOI:https://doi.org/10.1091/mbc.e11-01-0009· OSTI ID:1625225

Kang, Jungseog ^[1]; Chaudhary, Jaideep ^[2]; Dong, Hui ^[2]; Kim, Soonjoung ^[2]; Brautigam, Chad A. ^[3]; Yu, Hongtao ^[2]

Univ. of Texas Southwestern Medical Center, Dallas, TX (United States). Howard Hughes Medical Inst. Dept. of Pharmacology; DOE/OSTI
Univ. of Texas Southwestern Medical Center, Dallas, TX (United States). Howard Hughes Medical Inst. Dept. of Pharmacology
Univ. of Texas Southwestern Medical Center, Dallas, TX (United States). Dept. of Biochemistry

Human Shugoshin 1 (Sgo1) protects centromeric sister-chromatid cohesion during prophase and prevents premature sister-chromatid separation. Heterochromatin protein 1 (HP1) has been proposed to protect centromeric sister-chromatid cohesion by directly targeting Sgo1 to centromeres in mitosis. Here we show that HP1α is targeted to mitotic centromeres by INCENP, a subunit of the chromosome passenger complex (CPC). Biochemical and structural studies show that both HP1–INCENP and HP1–Sgo1 interactions require the binding of the HP1 chromo shadow domain to PXVXL/I motifs in INCENP or Sgo1, suggesting that the INCENP-bound, centromeric HP1α is incapable of recruiting Sgo1. Consistently, a Sgo1 mutant deficient in HP1 binding is functional in centromeric cohesion protection and localizes normally to centromeres in mitosis. By contrast, INCENP or Sgo1 mutants deficient in HP1 binding fail to localize to centromeres in interphase. Therefore, our results suggest that HP1 binding by INCENP or Sgo1 is dispensable for centromeric cohesion protection during mitosis of human cells, but might regulate yet uncharacterized interphase functions of CPC or Sgo1 at the centromeres.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Lab. (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1625225

Alternate ID(s):: OSTI ID: 1869837
OSTI ID: 1872635

Journal Information:: Molecular Biology of the Cell, Journal Name: Molecular Biology of the Cell Journal Issue: 8 Vol. 22; ISSN 1059-1524

Publisher:: American Society for Cell BiologyCopyright Statement

Country of Publication:: United States

Language:: English

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