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Rho-GTPase–dependent filamentous actin dynamics coordinate vesicle targeting and exocytosis during tip growth

Journal Article · · Journal of Cell Biology
 [1];  [2];  [2];  [3]
  1. Univ. of California, Riverside, CA (United States). Inst. for Integrative Genome Biology. Center for Plant Cell Biology. Dept. of Botany and Plant Sciences; DOE/OSTI
  2. Univ. of Michigan, Ann Arbor, MI (United States). Dept. of Molecular, Cellular and Developmental Biology
  3. Univ. of California, Riverside, CA (United States). Inst. for Integrative Genome Biology. Center for Plant Cell Biology. Dept. of Botany and Plant Sciences
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The dynamic activity of tip-localized filamentous actin (F-actin) in pollen tubes is controlled by counteracting RIC4 and RIC3 pathways downstream of the ROP1 guanosine triphosphatase promoting actin assembly and disassembly, respectively. We show here that ROP1 activation is required for both the polar accumulation and the exocytosis of vesicles at the plasma membrane apex. The apical accumulation of exocytic vesicles oscillated in phase with, but slightly behind, apical actin assembly and was enhanced by overexpression of RIC4. However, RIC4 overexpression inhibited exocytosis, and this inhibition could be suppressed by latrunculin B treatment or RIC3 overexpression. We conclude that RIC4-dependent actin assembly is required for polar vesicle accumulation, whereas RIC3-mediated actin disassembly is required for exocytosis. Thus ROP1-dependent F-actin dynamics control tip growth through spatiotemporal coordination of vesicle targeting and exocytosis.
Research Organization:
Donald Danforth Plant Science Center, St. Louis, MO (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division; National Science Foundation (NSF)
Grant/Contract Number:
FG02-03ER15412
OSTI ID:
1625148
Journal Information:
Journal of Cell Biology, Journal Name: Journal of Cell Biology Journal Issue: 7 Vol. 181; ISSN 0021-9525
Publisher:
Rockefeller University PressCopyright Statement
Country of Publication:
United States
Language:
English

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Arabidopsis FIM5 decorates apical actin filaments and regulates their organization in the pollen tube journal April 2016
Small GTPase patterning: How to stabilise cluster coexistence posted_content January 2018
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Polar Expansion Dynamics in the Plant Kingdom: A Diverse and Multifunctional Journey on the Path to Pollen Tubes journal March 2013
Calcium: The Missing Link in Auxin Action journal October 2013
Signaling in Pollen Tube Growth: Beyond the Tip of the Polarity Iceberg journal June 2019
ANXUR Receptor-Like Kinases Coordinate Cell Wall Integrity with Growth at the Pollen Tube Tip Via NADPH Oxidases text January 2013
Constrained Nonlinear and Mixed Effects Differential Equation Models for Dynamic Cell Polarity Signaling preprint January 2016
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The pollen tube clear zone: Clues to the mechanism of polarized growth: Differential organelle movement creates a clear zone journal January 2015
At the intersection of exocytosis and endocytosis in plants journal August 2019
ROP GTPase-Dependent Actin Microfilaments Promote PIN1 Polarization by Localized Inhibition of Clathrin-Dependent Endocytosis journal April 2012
ANXUR Receptor-Like Kinases Coordinate Cell Wall Integrity with Growth at the Pollen Tube Tip Via NADPH Oxidases journal November 2013
Glycolysis regulates pollen tube polarity via Rho GTPase signaling journal April 2018
Small GTPase patterning: How to stabilise cluster coexistence journal March 2019
ARF-GTPase activating protein mediates auxin influx carrier AUX1 early endosome trafficking to regulate auxin dependent plant development journal November 2011
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ANXUR receptor-like kinases coordinate cell wall integrity with growth at the pollen tube tip via NADPH oxidases text January 2013

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