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Evidence for a stem cell hierarchy in the adult human breast

Journal Article · · Journal of Cell Biology
 [1];  [2];  [3];  [4];  [5];  [6];  [6];  [2]
  1. Univ. of Copenhagen (Denmark). Faculty of Health Sciences. Dept. of Cellular and Molecular Medicine; DOE/OSTI
  2. Univ. of Copenhagen (Denmark). Faculty of Health Sciences. Dept. of Cellular and Molecular Medicine
  3. Univ. of Copenhagen (Denmark). Dept. of Molecular Biology. Zoophysiological Lab
  4. Univ. of Iceland, Reykjavik, (Iceland). Icelandic Cancer Society. Faculty of Medicine. Molecular and Cell Biology Research Lab.
  5. Univ. of Copenhagen (Denmark). State Univ. Hospital. Dept. of Pathology
  6. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Life Sciences Division
+ Show Author Affiliations
Cellular pathways that contribute to adult human mammary gland architecture and lineages have not been previously described. In this study, we identify a candidate stem cell niche in ducts and zones containing progenitor cells in lobules. Putative stem cells residing in ducts were essentially quiescent, whereas the progenitor cells in the lobules were more likely to be actively dividing. Cells from ducts and lobules collected under the microscope were functionally characterized by colony formation on tissue culture plastic, mammosphere formation in suspension culture, and morphogenesis in laminin-rich extracellular matrix gels. Staining for the lineage markers keratins K14 and K19 further revealed multipotent cells in the stem cell zone and three lineage-restricted cell types outside this zone. Multiparameter cell sorting and functional characterization with reference to anatomical sites in situ confirmed this pattern. The proposal that the four cell types are indeed constituents of an as of yet undescribed stem cell hierarchy was assessed in long-term cultures in which senescence was bypassed. These findings identify an adult human breast ductal stem cell activity and its earliest descendants.
Research Organization:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). National Energy Research Scientific Computing Center (NERSC)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
Grant/Contract Number:
AC02-05CH11231
OSTI ID:
1625142
Journal Information:
Journal of Cell Biology, Journal Name: Journal of Cell Biology Journal Issue: 1 Vol. 177; ISSN 0021-9525
Publisher:
Rockefeller University PressCopyright Statement
Country of Publication:
United States
Language:
English

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Additional file 1 of Prospective isolation and characterization of committed and multipotent progenitors from immortalized mouse mammary epithelial cells with morphogenic potential image January 2020
Additional file 2 of Prospective isolation and characterization of committed and multipotent progenitors from immortalized mouse mammary epithelial cells with morphogenic potential image January 2020
Additional file 3 of Prospective isolation and characterization of committed and multipotent progenitors from immortalized mouse mammary epithelial cells with morphogenic potential image January 2020
Additional file 4 of Prospective isolation and characterization of committed and multipotent progenitors from immortalized mouse mammary epithelial cells with morphogenic potential image January 2020
Additional file 5 of Prospective isolation and characterization of committed and multipotent progenitors from immortalized mouse mammary epithelial cells with morphogenic potential image January 2020
Additional file 6 of Prospective isolation and characterization of committed and multipotent progenitors from immortalized mouse mammary epithelial cells with morphogenic potential image January 2020
Additional file 7 of Prospective isolation and characterization of committed and multipotent progenitors from immortalized mouse mammary epithelial cells with morphogenic potential image January 2020
Additional file 2 of Impact of media compositions and culture systems on the immunophenotypes of patient-derived breast cancer cells image January 2023
Additional file 3 of Impact of media compositions and culture systems on the immunophenotypes of patient-derived breast cancer cells image January 2023
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