DaPKC-dependent phosphorylation of Crumbs is required for epithelial cell polarity in Drosophila

Sotillos, Sol; Díaz-Meco, María Teresa; Caminero, Eva; Moscat, Jorge; Campuzano, Sonsoles

doi:10.1083/jcb.200311031

DaPKC-dependent phosphorylation of Crumbs is required for epithelial cell polarity in Drosophila

Journal Article · Mon Aug 09 00:00:00 EDT 2004 · Journal of Cell Biology

DOI:https://doi.org/10.1083/jcb.200311031· OSTI ID:1625133

Sotillos, Sol ^[1]; Díaz-Meco, María Teresa ^[2]; Caminero, Eva ^[2]; Moscat, Jorge ^[2]; Campuzano, Sonsoles ^[2]

Consejo Superior de Investigaciones Científicas, Madrid (Spain). Centro de Biología Molecular Severo Ochoa; Universidad Autonóma de Madrid, Cantoblanco, Madrid (Spain); DOE/OSTI
Consejo Superior de Investigaciones Científicas, Madrid (Spain). Centro de Biología Molecular Severo Ochoa; Universidad Autonóma de Madrid, Cantoblanco, Madrid (Spain)

Both in Drosophila and vertebrate epithelial cells, the establishment of apicobasal polarity requires the apically localized, membrane-associated Par-3–Par-6–aPKC protein complex. In Drosophila, this complex colocalizes with the Crumbs–Stardust (Sdt)–Pals1-associated TJ protein (Patj) complex. Genetic and molecular analyses suggest a functional relationship between them. In this work we show, by overexpression of a kinase-dead Drosophila atypical PKC (DaPKC), the requirement for the kinase activity of DaPKC to maintain the position of apical determinants and to restrict the localization of basolateral ones. We demonstrate a novel physical interaction between the apical complexes, via direct binding of DaPKC to both Crb and Patj, and identify Crumbs as a phosphorylation target of DaPKC. This phosphorylation of Crumbs is functionally significant. Thus, a nonphosphorylatable Crumbs protein behaves in vivo as a dominant negative. Moreover, the phenotypic effect of overexpressing wild-type Crumbs is suppressed by reducing DaPKC activity. These results provide a mechanistic framework for the functional interaction between the Par-3–Par-6–aPKC and Crumbs–Sdt–Patj complexes based in the posttranslational modification of Crb by DaPKC.

View Accepted Manuscript (DOE)

Research Organization:: Consejo Superior de Investigaciones Cientificas (CSIC), Madrid (Spain); Universidad Autonóma de Madrid, Cantoblanco, Madrid (Spain)

Sponsoring Organization:: Comunidad Autónoma de Madrid (CAM); Dirección General de Investigación Científica y Técnica; Fundación Ramón Areces

OSTI ID:: 1625133

Journal Information:: Journal of Cell Biology, Journal Name: Journal of Cell Biology Journal Issue: 4 Vol. 166; ISSN 0021-9525

Publisher:: Rockefeller University PressCopyright Statement

Country of Publication:: United States

Language:: English

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Yorkie and JNK revert syncytial muscles into myoblasts during Org-1 dependent lineage reprogramming Schaub, Christoph; Rose, Marcel; Frasch, Manfred https://doi.org/10.1101/607820	posted_content	January 2019
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Atf3 links loss of epithelial polarity to defects in cell differentiation and cytoarchitecture Donohoe, Colin D.; Csordás, Gábor; Correia, Andreia PLOS Genetics, Vol. 14, Issue 3 https://doi.org/10.1371/journal.pgen.1007241	journal	March 2018
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Casein kinase 1 family proteins promote Slimb-dependent Expanded degradation. Fulford, Alexander D.; Holder, Maxine V.; Frith, David The Francis Crick Institute https://doi.org/10.25418/crick.11637006.v1	text	January 2020
Drosophila models of cell polarity and cell competition in tumourigenesis Fahey-Lozano, Natalia; La-Marca, John; Portela-Esteban, Marta La Trobe https://doi.org/10.26181/19305878.v3	other	January 2022
Enteropathogenic Escherichia coli (EPEC) Recruitment of PAR Polarity Protein Atypical PKCζ to Pedestals and Cell–Cell Contacts Precedes Disruption of Tight Junctions in Intestinal Epithelial Cells Tapia, Rocio; Kralicek, Sarah E.; Hecht, Gail A. International Journal of Molecular Sciences, Vol. 21, Issue 2 https://doi.org/10.3390/ijms21020527	journal	January 2020
Casein kinase 1 family proteins promote Slimb-dependent Expanded degradation Fulford, Alexander D.; Holder, Maxine V.; Frith, David eLife, Vol. 8 https://doi.org/10.7554/elife.46592	journal	September 2019
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59 BASIC BIOLOGICAL SCIENCES
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signal transduction

DaPKC-dependent phosphorylation of Crumbs is required for epithelial cell polarity in Drosophila

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