Drosophila aPKC regulates cell polarity and cell proliferation in neuroblasts and epithelia

Rolls, Melissa M.; Albertson, Roger; Shih, Hsin-Pei; Lee, Cheng-Yu; Doe, Chris Q.

doi:10.1083/jcb.200306079

Drosophila aPKC regulates cell polarity and cell proliferation in neuroblasts and epithelia

Journal Article · Mon Dec 01 00:00:00 EST 2003 · Journal of Cell Biology

DOI:https://doi.org/10.1083/jcb.200306079· OSTI ID:1625129

Rolls, Melissa M. ^[1]; Albertson, Roger ^[2]; Shih, Hsin-Pei ^[2]; Lee, Cheng-Yu ^[2]; Doe, Chris Q. ^[2]

Univ. of Oregon, Eugene, OR (United States). Howard Hughes Medical Institute, Institutes of Neuroscience and Molecular Biology; DOE/OSTI
Univ. of Oregon, Eugene, OR (United States). Howard Hughes Medical Institute, Institutes of Neuroscience and Molecular Biology

Cell polarity is essential for generating cell diversity and for the proper function of most differentiated cell types. In many organisms, cell polarity is regulated by the atypical protein kinase C (aPKC), Bazooka (Baz/Par3), and Par6 proteins. Here, we show that Drosophila aPKC zygotic null mutants survive to mid-larval stages, where they exhibit defects in neuroblast and epithelial cell polarity. Mutant neuroblasts lack apical localization of Par6 and Lgl, and fail to exclude Miranda from the apical cortex; yet, they show normal apical crescents of Baz/Par3, Pins, Inscuteable, and Discs large and normal spindle orientation. Mutant imaginal disc epithelia have defects in apical/basal cell polarity and tissue morphology. In addition, we show that aPKC mutants demonstrate reduced cell proliferation in both neuroblasts and epithelia, the opposite of the lethal giant larvae (lgl) tumor suppressor phenotype, and that reduced aPKC levels strongly suppress most lgl cell polarity and overproliferation phenotypes.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of Oregon, Eugene, OR (United States)

Sponsoring Organization:: Howard Hughes Medical Institute; National Institutes of Health (NIH); USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

OSTI ID:: 1625129

Journal Information:: Journal of Cell Biology, Journal Name: Journal of Cell Biology Journal Issue: 5 Vol. 163; ISSN 0021-9525

Publisher:: Rockefeller University PressCopyright Statement

Country of Publication:: United States

Language:: English

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A polybasic domain in aPKC mediates Par6-dependent control of membrane targeting and kinase activity Dong, Wei; Lu, Juan; Zhang, Xuejing Journal of Cell Biology, Vol. 219, Issue 7 https://doi.org/10.1083/jcb.201903031	journal	June 2020
Troponin-I localizes selected apico-basal cell polarity signals Casas-Tinto, Sergio; Ferrus, Alberto Journal of Cell Science https://doi.org/10.1101/404863	posted_content	August 2018
A Polybasic Domain in aPKC Mediates Par-6-Dependent Control of Plasma Membrane Targeting and Kinase Activity Dong, Wei; Lu, Juan; Zhang, Xuejing https://doi.org/10.1101/588624	posted_content	January 2019
AKT activation by N-cadherin regulates beta-catenin signaling and neuronal differentiation during cortical development Zhang, Jianing; Shemezis, Julie R.; McQuinn, Erin R. Neural Development, Vol. 8, Issue 1 https://doi.org/10.1186/1749-8104-8-7	journal	January 2013
A chemical-genetics approach to study the role of atypical protein kinase C in Drosophila Hannaford, Matthew; Loyer, Nicolas; Tonelli, Francesca Development https://doi.org/10.1242/dev.170589	journal	January 2019
Troponin-I localizes selected apico-basal cell polarity signals Casas-Tintó, Sergio; Ferrús, Alberto Journal of Cell Science https://doi.org/10.1242/jcs.225243	journal	January 2019
Myc and the Tip60 chromatin remodeling complex control neuroblast maintenance and polarity in Drosophila Rust, Katja; Tiwari, Manu D.; Mishra, Vivek Kumar The EMBO Journal, Vol. 37, Issue 16 https://doi.org/10.15252/embj.201798659	journal	July 2018
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Molecular and cell biology of brain tumor stem cells: lessons from neural progenitor/stem cells Xie, Zhigang; Chin, Lawrence S. Neurosurgical Focus, Vol. 24, Issue 3-4 https://doi.org/10.3171/foc/2008/24/3-4/e24	journal	March 2008
Cell Fate Decision Making through Oriented Cell Division Dewey, Evan; Taylor, Danielle; Johnston, Christopher Journal of Developmental Biology, Vol. 3, Issue 4 https://doi.org/10.3390/jdb3040129	journal	December 2015
Par6 is phosphorylated by aPKC to facilitate EMT Gunaratne, Adrian; Di Guglielmo, Gianni M. Cell Adhesion & Migration, Vol. 7, Issue 4 https://doi.org/10.4161/cam.25651	journal	July 2013
aPKC-mediated displacement and actomyosin-mediated retention polarize Miranda in Drosophila neuroblasts Hannaford, Matthew Robert; Ramat, Anne; Loyer, Nicolas eLife, Vol. 7 https://doi.org/10.7554/elife.29939	journal	January 2018

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59 BASIC BIOLOGICAL SCIENCES
Lgl
Miranda
Par complex
apical/basal polarity
asymmetric cell division
cell biology

Drosophila aPKC regulates cell polarity and cell proliferation in neuroblasts and epithelia

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