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The protein storage vacuole: a unique compound organelle

Journal Article · · Journal of Cell Biology
 [1];  [2];  [3];  [4];  [4];  [5];  [3];  [3]
  1. The Chinese Univ. of Hong Kong, Shatin, New Territories, Hong Kong (China). Dept. of Biology; DOE/OSTI
  2. Univ. of Missouri, Columbia, MO (United States). Division of Biological Sciences
  3. Washington State Univ., Pullman, WA (United States). Inst. of Biological Chemistry
  4. Univ. of Pennsylvania, Philadelphia, PA (United States). Plant Science Institute, Department of Biology
  5. Nagoya Univ., Chikusa-ku, Nagoya (Japan). Graduate School of Bioagricultural Science
+ Show Author Affiliations
Storage proteins are deposited into protein storage vacuoles (PSVs) during plant seed development and maturation and stably accumulate to high levels; subsequently, during germination the storage proteins are rapidly degraded to provide nutrients for use by the embryo. Here, we show that a PSV has within it a membrane-bound compartment containing crystals of phytic acid and proteins that are characteristic of a lytic vacuole. This compound organization, a vacuole within a vacuole whereby storage functions are separated from lytic functions, has not been described previously for organelles within the secretory pathway of eukaryotic cells. The partitioning of storage and lytic functions within the same vacuole may reflect the need to keep the functions separate during seed development and maturation and yet provide a ready source of digestive enzymes to initiate degradative processes early in germination.
Research Organization:
Washington State Univ., Pullman, WA (United States)
Sponsoring Organization:
Human Frontier Science Program; National Science Foundation (NSF); Research Grants Council (RGC); USDA; USDOE
Grant/Contract Number:
FG02-91ER20055; FG03-97ER20277
OSTI ID:
1625125
Journal Information:
Journal of Cell Biology, Journal Name: Journal of Cell Biology Journal Issue: 6 Vol. 155; ISSN 0021-9525
Publisher:
Rockefeller University PressCopyright Statement
Country of Publication:
United States
Language:
English

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Cited By (19)

Evolution of vacuolar proton pyrophosphatase domains and volutin granules: clues into the early evolutionary origin of the acidocalcisome journal January 2011
Reprogramming cells to study vacuolar development journal January 2013
Molecular Advances on Phytases in Barley and Wheat journal May 2019
Live Cell Imaging During Germination Reveals Dynamic Tubular Structures Derived from Protein Storage Vacuoles of Barley Aleurone Cells journal September 2014
Ultrastructural changes of pistachio (Pistacia vera L.) mature seeds and pollen in relation to desiccation journal August 2017
The formation, function and fate of protein storage compartments in seeds journal May 2011
Proteomic Profiling of the Aleurone Layer of Mature Arabidopsis thaliana Seed journal August 2012
Acidocalcisomes ? conserved from bacteria to man journal March 2005
Programmed cell death eliminates all but one embryo in a polyembryonic plant seed journal October 2002
Mobilization of vacuolar iron by AtNRAMP3 and AtNRAMP4 is essential for seed germination on low iron: Iron mobilization during seed germination journal November 2005
Canola/rapeseed protein-functionality and nutrition journal July 2016
Proteins of Brassicaceae Oilseeds and their Potential as a Plant Protein Source journal August 2011
Stubborn GFPs in Nicotiana tabacum vacuoles journal March 2004
New insights into globoids of protein storage vacuoles in wheat aleurone using synchrotron soft X-ray microscopy journal March 2011
ARA7(Q69L) expression in transgenic Arabidopsis cells induces the formation of enlarged multivesicular bodies journal May 2013
Trafficking routes to the plant vacuole: connecting alternative and classical pathways journal October 2017
Characterization of a novel organelle in Toxoplasma gondii with similar composition and function to the plant vacuole: Toxoplasma gondii plant-like vacuole journal April 2010
A role for caleosin in degradation of oil-body storage lipid during seed germination journal September 2006
Mobilization of seed protein reserves journal December 2011

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