Establishment of the Dorsal–Ventral Axis inXenopus Embryos Coincides with the Dorsal Enrichment of Dishevelled That Is Dependent on Cortical Rotation

Miller, Jeffrey R.; Rowning, Brian A.; Larabell, Carolyn A.; Yang-Snyder, Julia A.; Bates, Rebecca L.; Moon, Randall T.

doi:10.1083/jcb.146.2.427

Establishment of the Dorsal–Ventral Axis inXenopus Embryos Coincides with the Dorsal Enrichment of Dishevelled That Is Dependent on Cortical Rotation

Journal Article · Mon Jul 26 00:00:00 EDT 1999 · Journal of Cell Biology

DOI:https://doi.org/10.1083/jcb.146.2.427· OSTI ID:1625112

Miller, Jeffrey R. ^[1]; Rowning, Brian A. ^[2]; Larabell, Carolyn A. ^[3]; Yang-Snyder, Julia A. ^[4]; Bates, Rebecca L. ^[4]; Moon, Randall T. ^[4]

Univ. of Washington, Seattle, WA (United States). Howard Hughes Medical Inst. Center for Developmental Biology. Dept. of Pharmacology; DOE/OSTI
Univ. of Washington, Seattle, WA (United States). Howard Hughes Medical Inst. Center for Developmental Biology. Dept. of Pharmacology; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Univ. of Washington, Seattle, WA (United States). Howard Hughes Medical Inst. Center for Developmental Biology. Dept. of Pharmacology

Examination of the subcellular localization of Dishevelled (Dsh) in fertilized Xenopus eggs revealed that Dsh is associated with vesicle-like organelles that are enriched on the prospective dorsal side of the embryo after cortical rotation. Dorsal enrichment of Dsh is blocked by UV irradiation of the vegetal pole, a treatment that inhibits development of dorsal cell fates, linking accumulation of Dsh and specification of dorsal cell fates. Investigation of the dynamics of Dsh localization using Dsh tagged with green fluorescent protein (Dsh-GFP) demonstrated that Dsh-GFP associates with small vesicle-like organelles that are directionally transported along the parallel array of microtubules towards the prospective dorsal side of the embryo during cortical rotation. Perturbing the assembly of the microtubule array with D2O, a treatment that promotes the random assembly of the array and the dorsalization of embryos, randomizes translocation of Dsh-GFP. Conversely, UV irradiation of the vegetal pole abolishes movement of Dsh-GFP. Finally, we demonstrate that overexpression of Dsh can stabilize β-catenin in Xenopus. These data suggest that the directional translocation of Dsh along microtubules during cortical rotation and its subsequent enrichment on the prospective dorsal side of the embryo play a role in locally activating a maternal Wnt pathway responsible for establishing dorsal cell fates in Xenopus.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). National Energy Research Scientific Computing Center (NERSC)

Sponsoring Organization:: National Institutes of Health (NIH); USDOD; USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1625112

Journal Information:: Journal of Cell Biology, Journal Name: Journal of Cell Biology Journal Issue: 2 Vol. 146; ISSN 0021-9525

Publisher:: Rockefeller University PressCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
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Establishment of the Dorsal–Ventral Axis inXenopus Embryos Coincides with the Dorsal Enrichment of Dishevelled That Is Dependent on Cortical Rotation

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