Structural and functional differences between porcine brain and budding yeast microtubules

Howes, Stuart C.; Geyer, Elisabeth A.; LaFrance, Benjamin; Zhang, Rui; Kellogg, Elizabeth H.; Westermann, Stefan; Rice, Luke M.; Nogales, Eva

doi:10.1080/15384101.2017.1415680

Structural and functional differences between porcine brain and budding yeast microtubules

Journal Article · Tue Jan 30 00:00:00 EST 2018 · Cell Cycle (Georgetown, Tex)

DOI:https://doi.org/10.1080/15384101.2017.1415680· OSTI ID:1625099

^[1]; Geyer, Elisabeth A. ^[2]; LaFrance, Benjamin ^[3]; Zhang, Rui ^[4]; Kellogg, Elizabeth H. ^[5]; Westermann, Stefan ^[6]; Rice, Luke M. ^[2]; ^[7]

Univ. of California, Berkeley, CA (United States). Biophysics Graduate Group and Leiden Univ.Medical Center (Netherlands), Department of Molecular Cell Biology; DOE/OSTI
UT Southwestern Medical Center, Departments of Biophysics and Biochemistry, Dallas, TX 75390, USA
Molecular and Cell Biology Graduate Program, UC Berkeley, CA 94720, USA
Molecular Biophysics and Integrated Bioimaging, Lawrence Berkeley National Laboratory, CA 94720, USA; Howard Hughes Medical Institute, UC Berkeley, CA 94720-3220, USA; Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, MO 63110, USA
Molecular Biophysics and Integrated Bioimaging, Lawrence Berkeley National Laboratory, CA 94720, USA; Howard Hughes Medical Institute, UC Berkeley, CA 94720-3220, USA
Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, 1030 Vienna, Austria
Molecular Biophysics and Integrated Bioimaging, Lawrence Berkeley National Laboratory, CA 94720, USA; Howard Hughes Medical Institute, UC Berkeley, CA 94720-3220, USA; Molecular and Cell Biology Department and QB3 Institute, UC Berkeley, CA 94720, USA

The cytoskeleton of eukaryotic cells relies on microtubules to perform many essential functions. We have previously shown that, in spite of the overall conservation in sequence and structure of tubulin subunits across species, there are differences between mammalian and budding yeast microtubules with likely functional consequences for the cell. Here we expand our structural and function comparison of yeast and porcine microtubules to show different distribution of protofilament number in microtubules assembled in vitro from these two species. The different geometry at lateral contacts between protofilaments is likely due to a more polar interface in yeast. We also find that yeast tubulin forms longer and less curved oligomers in solution, suggesting stronger tubulin:tubulin interactions along the protofilament. Finally, we observed species-specific plus-end tracking activity for EB proteins: yeast Bim1 tracked yeast but not mammalian MTs, and human EB1 tracked mammalian but not yeast MTs. These findings further demonstrate that subtle sequence differences in tubulin sequence can have significant structural and functional consequences in microtubule structure and behavior.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1625099

Journal Information:: Cell Cycle (Georgetown, Tex), Journal Name: Cell Cycle (Georgetown, Tex) Journal Issue: 3 Vol. 17; ISSN 1538-4101

Publisher:: Taylor and FrancisCopyright Statement

Country of Publication:: United States

Language:: English

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