Increased Binding of Stroke-Induced Long Non-Coding RNAs to the Transcriptional Corepressors Sin3A and coREST

Dharap, Ashutosh; Pokrzywa, Courtney; Vemuganti, Raghu

doi:10.1042/an20130029

Title: Increased Binding of Stroke-Induced Long Non-Coding RNAs to the Transcriptional Corepressors Sin3A and coREST

Journal Article · Wed Aug 07 00:00:00 EDT 2013 · ASN Neuro

DOI:https://doi.org/10.1042/an20130029· OSTI ID:1624987

Dharap, Ashutosh ^[1]; Pokrzywa, Courtney ^[2]; Vemuganti, Raghu ^[2]

Univ. of Wisconsin, Madison, WI (United States). Dept. of Neurological Surgery; Los Alamos National Lab. (LANL), Los Alamos, NM (United States). Theoretical Biology and Biophysics (T-6)
Univ. of Wisconsin, Madison, WI (United States). Dept. of Neurological Surgery

LncRNAs (long non-coding RNAs) are thought to play a significant role in cellular homeostasis during development and disease by interacting with CMPs (chromatin-modifying proteins). We recently showed that following transient focal ischemia, the expression of many lncRNAs was altered significantly in rat brain. We currently analyzed whether focal ischemia also alters the association of lncRNAs with the CMPs Sin3A and coREST (corepressors of the RE-1 silencing transcription factor). RIP (RNA immunoprecipitation) combined with lncRNA microarray analysis showed that 177 of the 2497 lncRNAs expressed in rat cerebral cortex showed significantly increased binding to either Sin3A or coREST following ischemia compared with sham. Of these, 26 lncRNAs enriched with Sin3A and 11 lncRNAs enriched with coREST were also up-regulated in their expressions after ischemia. A majority of the lncRNAs enriched with these CMPs were intergenic in origin. Evaluation of the expression profiles of corresponding protein-coding genes showed that their expression levels correlate with those of the lncRNAs with which they shared a common locus. This is the first study to show that stroke-induced lncRNAs might associate with CMPs to modulate the post-ischemic epigenetic landscape.

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Research Organization:: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

Grant/Contract Number:: AC52-06NA25396

OSTI ID:: 1624987

Journal Information:: ASN Neuro, Vol. 5, Issue 4; ISSN 1759-0914

Publisher:: SAGECopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
Neurosciences & Neurology
cerebral ischemia
corepressor
genomics
long non-coding RNA
REST
Sin3A
transcription factor

Title: Increased Binding of Stroke-Induced Long Non-Coding RNAs to the Transcriptional Corepressors Sin3A and coREST

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