Green to red photoconversion of GFP for protein tracking in vivo

Sattarzadeh, Amirali; Saberianfar, Reza; Zipfel, Warren R.; Menassa, Rima; Hanson, Maureen R.

doi:10.1038/srep11771

Green to red photoconversion of GFP for protein tracking in vivo

Journal Article · Tue Jul 07 00:00:00 EDT 2015 · Scientific Reports

DOI:https://doi.org/10.1038/srep11771· OSTI ID:1624778

Sattarzadeh, Amirali ^[1]; Saberianfar, Reza ^[2]; Zipfel, Warren R. ^[3]; Menassa, Rima ^[4]; Hanson, Maureen R. ^[5]

Cornell University, Ithaca, 14853, NY (United States). Department of Molecular Biology and Genetics; DOE/OSTI
University of Western Ontario, London, ON (Canada). Dept. of Biology
Cornell University, Ithaca, 14853, NY (United States). Department of Biomedical Engineering
University of Western Ontario, London, ON (Canada). Department of Biology
Cornell University, Ithaca, 14853, NY (United States). Department of Molecular Biology and Genetics

A variety of fluorescent proteins have been identified that undergo shifts in spectral emission properties over time or once they are irradiated by ultraviolet or blue light. Such proteins are finding application in following the dynamics of particular proteins or labelled organelles within the cell. However, before genes encoding these fluorescent proteins were available, many proteins have already been labelled with GFP in transgenic cells; a number of model organisms feature collections of GFP-tagged lines and organisms. Here we describe a fast, localized and non-invasive method for GFP photoconversion from green to red. We demonstrate its use in transgenic plant, Drosophila and mammalian cells in vivo. While genes encoding fluorescent proteins specifically designed for photoconversion will usually be advantageous when creating new transgenic lines, our method for photoconversion of GFP allows the use of existing GFP-tagged transgenic lines for studies of dynamic processes in living cells.

View Accepted Manuscript (DOE)

Research Organization:: Cornell Univ., Ithaca, NY (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: SC0002628

OSTI ID:: 1624778

Journal Information:: Scientific Reports, Journal Name: Scientific Reports Journal Issue: 1 Vol. 5; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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