Pathogenic diversity amongst serotype C VGIII and VGIV Cryptococcus gattii isolates

Rodrigues, Jéssica; Fonseca, Fernanda L.; Schneider, Rafael O.; Godinho, Rodrigo M.  da C.; Firacative, Carolina; Maszewska, Krystyna; Meyer, Wieland; Schrank, Augusto; Staats, Charley; Kmetzsch, Livia; Vainstein, Marilene H.; Rodrigues, Marcio L.

doi:10.1038/srep11717

Pathogenic diversity amongst serotype C VGIII and VGIV Cryptococcus gattii isolates

Journal Article · Wed Jul 08 00:00:00 EDT 2015 · Scientific Reports

DOI:https://doi.org/10.1038/srep11717· OSTI ID:1624776

Rodrigues, Jéssica ^[1]; Fonseca, Fernanda L. ^[2]; Schneider, Rafael O. ^[3]; Godinho, Rodrigo M. da C. ^[4]; Firacative, Carolina ^[5]; Maszewska, Krystyna ^[6]; Meyer, Wieland ^[6]; Schrank, Augusto ^[3]; Staats, Charley ^[3]; Kmetzsch, Livia ^[3]; Vainstein, Marilene H. ^[3]; Rodrigues, Marcio L. ^[7]

Federal Univ. of Rio de Janeiro (Brazil). Inst. de Microbiologia de Goes; DOE/OSTI
Centro de Desenvolvimento Tecnológico em Saúde (CDTS), Rio de Janeiro (Brazil). Fundação Oswaldo Cruz – Fiocruz
Federal Univ. of Rio Grande do Sul, Porto Alegre (Brazil). Centro de Biotecnologia
Federal Univ. of Rio de Janeiro (Brazil). Inst. de Microbiologia de Goes
Univ. of Sydney, NSW (Australia). Sydney Medical School Westmead Hospital. Marie Bashir Inst. for Infectious Diseases and Biosecurity. Centre for Infectious Diseases and Microbiology. Molecular Mycology Research Lab.; Instituto Nacional de Salud, Bogotá (Colombia). Grupo de Microbiología
Univ. of Sydney, NSW (Australia). Sydney Medical School Westmead Hospital. Marie Bashir Inst. for Infectious Diseases and Biosecurity. Centre for Infectious Diseases and Microbiology. Molecular Mycology Research Lab.
Federal Univ. of Rio de Janeiro (Brazil). Inst. de Microbiologia de Goes; Centro de Desenvolvimento Tecnológico em Saúde (CDTS), Rio de Janeiro (Brazil). Fundação Oswaldo Cruz – Fiocruz

Cryptococcus gattii is one of the causative agents of human cryptococcosis. Highly virulent strains of serotype B C. gattii have been studied in detail, but little information is available on the pathogenic properties of serotype C isolates. In this study, we analyzed pathogenic determinants in three serotype C C. gattii isolates (106.97, ATCC 24066 and WM 779). Isolate ATCC 24066 (molecular type VGIII) differed from isolates WM 779 and 106.97 (both VGIV) in capsule dimensions, expression of CAP genes, chitooligomer distribution, and induction of host chitinase activity. Isolate WM 779 was more efficient than the others in producing pigments and all three isolates had distinct patterns of reactivity with antibodies to glucuronoxylomannan. This great phenotypic diversity reflected in differential pathogenicity. VGIV isolates WM 779 and 106.97 were similar in their ability to cause lethality and produced higher pulmonary fungal burden in a murine model of cryptococcosis, while isolate ATCC 24066 (VGIII) was unable to reach the brain and caused reduced lethality in intranasally infected mice. These results demonstrate a high diversity in the pathogenic potential of isolates of C. gattii belonging to the molecular types VGIII and VGIV.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of Sydney, NSW (Australia)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: SC0010687

OSTI ID:: 1624776

Journal Information:: Scientific Reports, Journal Name: Scientific Reports Journal Issue: 1 Vol. 5; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Highly recombinant VGII Cryptococcus gattii population develops clonal outbreak clusters through both sexual macroevolution and asexual microevolution Billmyre, R. Blake; Croll, Daniel; Li, Wenjun ETH Zurich https://doi.org/10.3929/ethz-b-000097185	text	January 2014

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The Anti-helminthic Compound Mebendazole Has Multiple Antifungal Effects against Cryptococcus neoformans Joffe, Luna S.; Schneider, Rafael; Lopes, William Frontiers in Microbiology, Vol. 8 https://doi.org/10.3389/fmicb.2017.00535	journal	March 2017
Differential In Vitro Cytokine Induction by the Species of Cryptococcus gattii Complex Herkert, Patricia F.; dos Santos, Jessica C.; Hagen, Ferry Infection and Immunity, Vol. 86, Issue 4 https://doi.org/10.1128/iai.00958-17	journal	January 2018

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