Formation of Pseudomonas aeruginosa Biofilms in Full-thickness Scald Burn Wounds in Rats

Brandenburg, Kenneth S.; Weaver, Alan J.; Karna, S. L.  Rajasekhar; You, Tao; Chen, Ping; Stryk, Shaina Van; Qian, Liwu; Pineda, Uzziel; Abercrombie, Johnathan J.; Leung, Kai P.

doi:10.1038/s41598-019-50003-8

Title: Formation of Pseudomonas aeruginosa Biofilms in Full-thickness Scald Burn Wounds in Rats

Journal Article · Fri Sep 20 00:00:00 EDT 2019 · Scientific Reports

DOI:https://doi.org/10.1038/s41598-019-50003-8· OSTI ID:1624491

Brandenburg, Kenneth S. ^[1]; Weaver, Alan J. ^[1]; Karna, S. L. Rajasekhar ^[1]; You, Tao ^[1]; Chen, Ping ^[1]; Stryk, Shaina Van ^[1]; Qian, Liwu ^[1]; Pineda, Uzziel ^[1]; Abercrombie, Johnathan J. ^[1]; Leung, Kai P. ^[1]

United States Army Institute of Surgical Research, JBSA Fort Sam, Houston, Texas, (United States). Dental and Craniofacial Trauma Research and Tissue Regeneration Dept.

Using Sprague-Dawley rats (350–450 g; n = 61) and the recently updated Walker-Mason rat scald burn model, we demonstrated that Pseudomonas aeruginosa readily formed biofilms within full-thickness burn wounds. Following the burn, wounds were surface-inoculated with P. aeruginosa in phosphate-buffered saline (PBS), while sterile PBS was used for controls. On post-burn days 1, 3, 7, and 11, animals were euthanized and samples collected for quantitative bacteriology, bacterial gene expression, complete blood cell counts, histology, and myeloperoxidase activity. Robust biofilm infections developed in the full-thickness burn wounds inoculated with 1 × 10⁴ CFU of P. aeruginosa. Both histology and scanning electron microscopy showed the pathogen throughout the histologic cross-sections of burned skin. Quantigene analysis revealed significant upregulation of alginate and pellicle biofilm matrix genes of P. aeruginosa within the burn eschar. Additionally, expression of P. aeruginosa proteases and siderophores increased significantly in the burn wound environment. Interestingly, the host’s neutrophil response to the pathogen was not elevated in either the eschar or circulating blood when compared to the control burn. This new full-thickness burn biofilm infection model will be used to test new anti-biofilm therapies that may be deployed with soldiers in combat for immediate use at the site of burn injury on the battlefield.

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Research Organization:: Oak Ridge Institute of Science and Education (ORISE), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC); Naval Medical Research Center’s Advanced Medical Development Program; Combat Casualty Care Research Directorate, US Army Medical Research and Development Command (USAMRDC); U.S. Army Institute of Surgical Research

Grant/Contract Number:: SC0014664; N3239815MHX040

OSTI ID:: 1624491

Journal Information:: Scientific Reports, Vol. 9, Issue 1; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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