Lignin Valorization: Two Hybrid Biochemical Routes for the Conversion of Polymeric Lignin into Value-added Chemicals

Wu, Weihua; Dutta, Tanmoy; Varman, Arul M.; Eudes, Aymerick; Manalansan, Bianca; Loqué, Dominique; Singh, Seema

doi:10.1038/s41598-017-07895-1

Lignin Valorization: Two Hybrid Biochemical Routes for the Conversion of Polymeric Lignin into Value-added Chemicals

Journal Article · Mon Aug 21 00:00:00 EDT 2017 · Scientific Reports

DOI:https://doi.org/10.1038/s41598-017-07895-1· OSTI ID:1624321

Wu, Weihua ^[1]; ^[2]; Varman, Arul M. ^[2]; Eudes, Aymerick ^[3]; Manalansan, Bianca ^[3]; Loqué, Dominique ^[3]; Singh, Seema ^[4]

Sandia National Lab. (SNL-CA), Livermore, CA (United States). Dept. of Biomass Science & Conversion Technologies; DOE/OSTI
Sandia National Lab. (SNL-CA), Livermore, CA (United States). Dept. of Biomass Science & Conversion Technologies
Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States)
Sandia National Lab. (SNL-CA), Livermore, CA (United States). Dept. of Biomass Science & Conversion Technologies; Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Univ. of Minnesota, Minneapolis, MN (United States). Dept. of Bioproducts and Biosystems Engineering

Naturally, many aerobic organisms degrade lignin-derived aromatics through conserved intermediates including protocatechuate and catechol. Employing this microbial approach offers a potential solution for valorizing lignin into valuable chemicals for a potential lignocellulosic biorefinery and enabling bioeconomy. In this study, two hybrid biochemical routes combining lignin chemical depolymerization, plant metabolic engineering, and synthetic pathway reconstruction were demonstrated for valorizing lignin into value-added products. In the biochemical route 1, alkali lignin was chemically depolymerized into vanillin and syringate as major products, which were further bio-converted into cis, cis-muconic acid (ccMA) and pyrogallol, respectively, using engineered Escherichia coli strains. In the second biochemical route, the shikimate pathway of Tobacco plant was engineered to accumulate protocatechuate (PCA) as a soluble intermediate compound. The PCA extracted from the engineered Tobacco was further converted into ccMA using the engineered E. coli strain. This study reports a direct process for converting lignin into ccMA and pyrogallol as value-added chemicals, and more importantly demonstrates benign methods for valorization of polymeric lignin that is inherently heterogeneous and recalcitrant. Our approach also validates the promising combination of plant engineering with microbial chassis development for the production of value added and speciality chemicals.

View Accepted Manuscript (DOE)

Research Organization:: Sandia National Laboratories (SNL-NM), Albuquerque, NM (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC04-94AL85000

OSTI ID:: 1624321

Journal Information:: Scientific Reports, Journal Name: Scientific Reports Journal Issue: 1 Vol. 7; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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