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A common assembly module in injectisome and flagellar type III secretion sorting platforms

Journal Article · · Nature Communications
DOI:https://doi.org/10.1038/ncomms8125· OSTI ID:1623975
 [1];  [2];  [3];  [3]
  1. Laboratory of Structural Microbiology, Rockefeller University, New York, NY (United States); Tri-Institutional Medical Scientist Training Program, Weill Cornell Medical College, New York, NY (United States); DOE/OSTI
  2. New York Structural Biology Center, New York, NY, (United States)
  3. Laboratory of Structural Microbiology, Rockefeller University, New York, NY (United States)
+ Show Author Affiliations
Translocating proteins across the double membrane of Gram-negative bacteria, type III secretion systems (T3SS) occur in two evolutionarily related forms: injectisomes, delivering virulence factors into host cells, and the flagellar system, secreting the polymeric filament used for motility. While both systems share related elements of a cytoplasmic sorting platform that facilitates the hierarchical secretion of protein substrates, its assembly and regulation remain unclear. Here we describe a module mediating the assembly of the sorting platform in both secretion systems, and elucidate the structural basis for segregation of homologous components among these divergent T3SS subtypes sharing a common cytoplasmic milieu. These results provide a foundation for the subtype-specific assembly of T3SS sorting platforms and will support further mechanistic analysis and anti-virulence drug design.
Research Organization:
Brookhaven National Lab (BNL) Brookhaven, NY (United States)
Sponsoring Organization:
National Center for Research Resources; National Institute of General Medical Sciences (NIGMS); National Institutes of Health (NIH); Rockefeller University Structural Biology Resource Center; The Keck Foundation, New York State Assembly; U.S. Department of Defense; USDOE Office of Science (SC)
Grant/Contract Number:
SC0012704
OSTI ID:
1623975
Journal Information:
Nature Communications, Journal Name: Nature Communications Journal Issue: 1 Vol. 6; ISSN 2041-1723
Publisher:
Nature Publishing GroupCopyright Statement
Country of Publication:
United States
Language:
English

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Cited By (19)

Directional Switching Mechanism of the Bacterial Flagellar Motor journal January 2019
The Architecture of the Cytoplasmic Region of Type III Secretion Systems journal September 2016
The flagellar motor of Vibrio alginolyticus undergoes major structural remodeling during rotational switching preprint April 2020
Role of SpaO in the assembly of the sorting platform of a Salmonella type III secretion system journal January 2019
Structural differences in the bacterial flagellar motor among bacterial species journal January 2017
Type Three Secretion System in Attaching and Effacing Pathogens journal October 2016
Super-Resolution Imaging of Protein Secretion Systems and the Cell Surface of Gram-Negative Bacteria journal May 2017
The Type III Secretion System of Pathogenic Escherichia coli book August 2018
Phonon dispersion evolution in uniaxially strained aluminum crystal journal April 2018
Three SpoA-domain proteins interact in the creation of the flagellar type III secretion system in Helicobacter pylori journal July 2018
Determination of the Stoichiometry of the Complete Bacterial Type III Secretion Needle Complex Using a Combined Quantitative Proteomic Approach journal February 2016
Bacterial type III secretion systems: a complex device for the delivery of bacterial effector proteins into eukaryotic host cells journal August 2018
Characterization of C-ring component assembly in flagellar motors from amino acid coevolution journal May 2018
Type III secretion systems: the bacterial flagellum and the injectisome journal October 2015
Structural modeling of the flagellum MS ring protein FliF reveals similarities to the type III secretion system and sporulation complex posted_content November 2015
ZomB is essential for flagellar motor reversals in Shewanella putrefaciens and Vibrio parahaemolyticus journal August 2018
A dual role for proline iminopeptidase in the regulation of bacterial motility and host immunity: A dual role for proline iminopeptidase journal April 2018
Diversification of Campylobacter jejuni Flagellar C-Ring Composition Impacts Its Structure and Function in Motility, Flagellar Assembly, and Cellular Processes journal January 2020
Structural modeling of the flagellum MS ring protein FliF reveals similarities to the type III secretion system and sporulation complex journal January 2016

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