Genome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica
- Max-Planck Inst. for Molecular Genetics, Berlin-Dahlem (Germany); Humboldt Univ. of Berlin (Germany). Dept. of Crop and Animal Science. Section Phytomedicine
- HZI—Helmholtz Centre for Infection Research, Braunschweig (Germany). Environmental Microbiology Group; Bangor Univ., Wales (United Kingdom). School of Biological Sciences
- Consejo Superior de Investigaciones Cientificas (CSIC), Madrid (Spain). Inst. of Catalysis
- Consejo Superior de Investigaciones Cientificas (CSIC), Madrid (Spain). Inst. of Catalysis; Univ. of Sao Paulo (Brazil). Dept. de Quimica
- Helmholtz Centre for Environmental Research-UFZ, Leipzig (Germany). Dept. of Environmental Biotechnology
- Max-Planck Inst. for Molecular Genetics, Berlin-Dahlem (Germany)
- HZI—Helmholtz Centre for Infection Research, Braunschweig (Germany). Environmental Microbiology Group
- Consejo Superior de Investigaciones Cientificas (CSIC), Madrid (Spain). National Centre for Biotechnology. Proteomic Facility
- Univ. of Toronto, ON (Canada). The Banting and Best Dept. of Medical Research; Argonne National Lab. (ANL), Argonne, IL (United States). Midwest Center for Structural Genomics. Biosciences Division
- Argonne National Lab. (ANL), Argonne, IL (United States). Midwest Center for Structural Genomics. Biosciences Division; Univ. of Toronto, ON (Canada). C. H. Best Inst. Dept. of Chemical Engineering and Applied Chemistry
- Univ. of Toronto, ON (Canada). C. H. Best Inst. Dept. of Chemical Engineering and Applied Chemistry
- Argonne National Lab. (ANL), Argonne, IL (United States). Midwest Center for Structural Genomics. Biosciences Division; Argonne National Lab. (ANL), Argonne, IL (United States). Structural Biology Center. Biosciences Division
- Univ. of Toronto, ON (Canada). The Banting and Best Dept. of Medical Research
- Inst. for Coastal Marine Environment (IAMC), CNR, Messina (Italy). Lab. of Marine Molecular Microbiology
- Univ. of Pretoria (South Africa). Dept. of Biochemsitry
- UCM—Complutense University Madrid (Spain). Proteomics Unit
- Bangor Univ., Wales (United Kingdom). School of Biological Sciences
- Univ. of Toronto, ON (Canada). The Banting and Best Dept. of Medical Research; Argonne National Lab. (ANL), Argonne, IL (United States). Midwest Center for Structural Genomics. Biosciences Division; Univ. of Toronto, ON (Canada). C.H. Best Inst. Dept. of Chemical Engineering and Applied Chemistry
- Univ. of Toronto, ON (Canada). C.H. Best Inst. Dept. of Chemical Engineering and Applied Chemistry
Ubiquitous bacteria from the genus Oleispira drive oil degradation in the largest environment on Earth, the cold and deep sea. Here we report the genome sequence of Oleispira antarctica and show that compared with Alcanivorax borkumensis—the paradigm of mesophilic hydrocarbonoclastic bacteria—O. antarctica has a larger genome that has witnessed massive gene-transfer events. We identify an array of alkane monooxygenases, osmoprotectants, siderophores and micronutrient-scavenging pathways. We also show that at low temperatures, the main protein-folding machine Cpn60 functions as a single heptameric barrel that uses larger proteins as substrates compared with the classical double-barrel structure observed at higher temperatures. With 11 protein crystal structures, we further report the largest set of structures from one psychrotolerant organism. The most common structural feature is an increased content of surface-exposed negatively charged residues compared to their mesophilic counterparts. Our findings are relevant in the context of microbial cold-adaptation mechanisms and the development of strategies for oil-spill mitigation in cold environments.
- Research Organization:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- Sponsoring Organization:
- USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division; European Union (EU); Genome Canada; National Institutes of Health (NIH); German Research Foundation (DFG)
- Grant/Contract Number:
- AC02-06CH11357; 226977; 266473; 245226; 287589; 2009-OGI-ABC-1405; GM074942; GM094585
- OSTI ID:
- 1623920
- Journal Information:
- Nature Communications, Vol. 4, Issue 1; ISSN 2041-1723
- Publisher:
- Nature Publishing GroupCopyright Statement
- Country of Publication:
- United States
- Language:
- English
Similar Records
Geochemical, metagenomic and metaproteomic insights into trace metal utilization by methane-oxidizing microbial consortia in sulfidic marine sediments
Corexit 9500 Enhances Oil Biodegradation and Changes Active Bacterial Community Structure of Oil-Enriched Microcosms