Classical non-homologous end-joining pathway utilizes nascent RNA for error-free double-strand break repair of transcribed genes

Chakraborty, Anirban; Tapryal, Nisha; Venkova, Tatiana; Horikoshi, Nobuo; Pandita, Raj K.; Sarker, Altaf H.; Sarkar, Partha S.; Pandita, Tej K.; Hazra, Tapas K.

doi:10.1038/ncomms13049

Classical non-homologous end-joining pathway utilizes nascent RNA for error-free double-strand break repair of transcribed genes

Journal Article · Wed Oct 05 00:00:00 EDT 2016 · Nature Communications

DOI:https://doi.org/10.1038/ncomms13049· OSTI ID:1623855

Chakraborty, Anirban ^[1]; Tapryal, Nisha ^[2]; Venkova, Tatiana ^[2]; Horikoshi, Nobuo ^[3]; Pandita, Raj K. ^[3]; Sarker, Altaf H. ^[4]; Sarkar, Partha S. ^[5]; Pandita, Tej K. ^[3]; Hazra, Tapas K. ^[2]

University of Texas Medical Branch, Galveston, Texas (United States). Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Sealy Center for Molecular Medicine; DOE/OSTI
University of Texas Medical Branch, Galveston, Texas (United States). Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Sealy Center for Molecular Medicine
The Houston Methodist Research Institute, Houston, Texas (United States). Department of Radiation Oncology, The Houston Methodist Research Institute
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Division of Life Sciences, Department of Cancer and DNA Damage Responses
University of Texas Medical Branch, Galveston, Texas (United States). Department of Neurology and Neuroscience and Cell Biology

DNA double-strand breaks (DSBs) leading to loss of nucleotides in the transcribed region can be lethal. Classical non-homologous end-joining (C-NHEJ) is the dominant pathway for DSB repair (DSBR) in adult mammalian cells. Here we report that during such DSBR, mammalian C-NHEJ proteins form a multiprotein complex with RNA polymerase II and preferentially associate with the transcribed genes after DSB induction. Depletion of C-NHEJ factors significantly abrogates DSBR in transcribed but not in non-transcribed genes. We hypothesized that nascent RNA can serve as a template for restoring the missing sequences, thus allowing error-free DSBR. We indeed found pre-mRNA in the C-NHEJ complex. Finally, when a DSB-containing plasmid with several nucleotides deleted within the E. coli lacZ gene was allowed time to repair in lacZ expressing mammalian cells, a functional lacZ plasmid could be recovered from control but not C-NHEJ factor-depleted cells, providing important mechanistic insights into C-NHEJ-mediated error-free DSBR of the transcribed genome.

View Accepted Manuscript (DOE)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1623855

Journal Information:: Nature Communications, Journal Name: Nature Communications Journal Issue: 1 Vol. 7; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Classical non-homologous end-joining pathway utilizes nascent RNA for error-free double-strand break repair of transcribed genes

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