A mutation in the AdhE alcohol dehydrogenase of Clostridium thermocellum increases tolerance to several primary alcohols, including isobutanol, n-butanol and ethanol

Tian, Liang; Cervenka, Nicholas D.; Low, Aidan M.; Olson, Daniel G.; Lynd, Lee R.

doi:10.1038/s41598-018-37979-5

A mutation in the AdhE alcohol dehydrogenase of Clostridium thermocellum increases tolerance to several primary alcohols, including isobutanol, n-butanol and ethanol

Journal Article · Mon Feb 11 00:00:00 EST 2019 · Scientific Reports

DOI:https://doi.org/10.1038/s41598-018-37979-5· OSTI ID:1619494

; Cervenka, Nicholas D.; Low, Aidan M.; ; Lynd, Lee R.

Abstract

Clostridium thermocellum is a good candidate organism for producing cellulosic biofuels due to its native ability to ferment cellulose, however its maximum biofuel titer is limited by tolerance. Wild type C . thermocellum is inhibited by 5 g/L n-butanol. Using growth adaptation in a chemostat, we increased n-butanol tolerance to 15 g/L. We discovered that several tolerant strains had acquired a D494G mutation in the adhE gene. Re-introducing this mutation recapitulated the n-butanol tolerance phenotype. In addition, it increased tolerance to several other primary alcohols including isobutanol and ethanol. To confirm that adhE is the cause of inhibition by primary alcohols, we showed that deleting adhE also increases tolerance to several primary alcohols.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE; USDOE Office of Science (SC), Biological and Environmental Research (BER), Joint Genome Institute

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1619494

Alternate ID(s):: OSTI ID: 1624446

Journal Information:: Scientific Reports, Journal Name: Scientific Reports Journal Issue: 1 Vol. 9; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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