Characterizing virus-induced gene silencing at the cellular level with in situ multimodal imaging

Burkhow, Sadie J.; Stephens, Nicole M.; Mei, Yu; Dueñas, Maria Emilia; Freppon, Daniel J.; Ding, Geng; Smith, Shea C.; Lee, Young-Jin; Nikolau, Basil J.; Whitham, Steven A.; Smith, Emily A.

doi:10.1186/s13007-018-0306-7

Title: Characterizing virus-induced gene silencing at the cellular level with in situ multimodal imaging

Journal Article · Fri May 25 00:00:00 EDT 2018 · Plant Methods

DOI:https://doi.org/10.1186/s13007-018-0306-7· OSTI ID:1618908

Burkhow, Sadie J.; Stephens, Nicole M.; Mei, Yu; Dueñas, Maria Emilia; Freppon, Daniel J.; Ding, Geng; Smith, Shea C.; Lee, Young-Jin; Nikolau, Basil J.; Whitham, Steven A.;

Reverse genetic strategies, such as virus-induced gene silencing, are powerful techniques to study gene function. Currently, there are few tools to study the spatial dependence of the consequences of gene silencing at the cellular level. Here, we report the use of multimodal Raman and mass spectrometry imaging to study the cellular-level biochemical changes that occur from silencing the phytoene desaturase (pds) gene using a Foxtail mosaic virus (FoMV) vector in maize leaves. The multimodal imaging method allows the localized carotenoid distribution to be measured and reveals differences lost in the spatial average when analyzing a carotenoid extraction of the whole leaf. The nature of the Raman and mass spectrometry signals are complementary: silencing pds reduces the downstream carotenoid Raman signal and increases the phytoene mass spectrometry signal.

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Research Organization:: Ames Laboratory (AMES), Ames, IA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: Contract No. DE-AC02-07CH11358; DESC0014038; AC02-07CH11358; SC0014038; Hatch Project No. 3808

OSTI ID:: 1618908

Alternate ID(s):: OSTI ID: 1440994

Report Number(s):: IS-J-9659; 37; PII: 306

Journal Information:: Plant Methods, Journal Name: Plant Methods Vol. 14 Journal Issue: 1; ISSN 1746-4811

Publisher:: Springer Science + Business MediaCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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Cited by: 9 works

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
RNA silencing
Foxtail mosaic virus
Phytoene desaturase
Subcellular Raman imaging
Mass spectrometry imaging
Whole-plant analysis
Biochemical characterization
Mosaic spatial pattern
Carotenoids

MOESM1 of Characterizing virus-induced gene silencing at the cellular level with in situ multimodal imaging Burkhow, Sadie; Stephens, Nicole; Mei, Yu https://doi.org/10.6084/m9.figshare.6362894 The current record is supplemented by this dataset	dataset	May 2018
MOESM1 of Characterizing virus-induced gene silencing at the cellular level with in situ multimodal imaging Burkhow, Sadie; Stephens, Nicole; Mei, Yu https://doi.org/10.6084/m9.figshare.6362894.v1 The current record is supplemented by this dataset	dataset	May 2018
Characterizing virus-induced gene silencing at the cellular level with in situ multimodal imaging Burkhow, Sadie; Stephens, Nicole; Mei, Yu https://doi.org/10.6084/m9.figshare.c.4113797 The current record is supplemented by this dataset	dataset	May 2018
Characterizing virus-induced gene silencing at the cellular level with in situ multimodal imaging [Supplemental Data] Burkhow, Sadie; Stephens, Nicole; Mei, Yu https://doi.org/10.6084/m9.figshare.c.4113797.v1 The current record is supplemented by this figure	figure	May 2018