Simultaneous knockdown of six non-family genes using a single synthetic RNAi fragment in Arabidopsis thaliana

Czarnecki, Olaf; Bryan, Anthony C.; Jawdy, Sara S.; Yang, Xiaohan; Cheng, Zong-Ming; Chen, Jin-Gui; Tuskan, Gerald A.

doi:10.1186/s13007-016-0116-8

Title: Simultaneous knockdown of six non-family genes using a single synthetic RNAi fragment in Arabidopsis thaliana

Journal Article · Wed Feb 17 00:00:00 EST 2016 · Plant Methods

DOI:https://doi.org/10.1186/s13007-016-0116-8· OSTI ID:1618904

Czarnecki, Olaf; Bryan, Anthony C.; Jawdy, Sara S.; Yang, Xiaohan; Cheng, Zong-Ming; Chen, Jin-Gui; Tuskan, Gerald A.

Genetic engineering of plants that results in successful establishment of new biochemical or regulatory pathways requires stable introduction of one or more genes into the plant genome. It might also be necessary to down-regulate or turn off expression of endogenous genes in order to reduce activity of competing pathways. An established way to knockdown gene expression in plants is expressing a hairpin-(hp)-RNAi construct, eventually leading to degradation of a specifically targeted mRNA. Ultimately, knockdown of multiple genes that do not share homologous sequences is still challenging and involves either sophisticated cloning strategies to create vectors with different serial expression constructs or multiple transformation events that is often restricted by a lack of available transformation markers.

View Journal Article

Cite

Export

Save

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: SC0008834; AC05-00OR22725

OSTI ID:: 1618904

Alternate ID(s):: OSTI ID: 1294077; OSTI ID: 1311232

Journal Information:: Plant Methods, Journal Name: Plant Methods Vol. 12 Journal Issue: 1; ISSN 1746-4811

Publisher:: Springer Science + Business MediaCopyright Statement

Country of Publication:: United Kingdom

Language:: English

Citation Metrics:

Cited by: 12 works

Citation information provided by
Web of Science

References (73)

Integrating C4 photosynthesis into C3 crops to increase yield potential Covshoff, Sarah; Hibberd, Julian M. Current Opinion in Biotechnology, Vol. 23, Issue 2 https://doi.org/10.1016/j.copbio.2011.12.011	journal	April 2012
Creation and validation of a widely applicable multiple gene transfer vector system for stable transformation in plant Sun, Quanxi; Liu, Jiang; Li, Yaxiao Plant Molecular Biology, Vol. 83, Issue 4-5 https://doi.org/10.1007/s11103-013-0096-2	journal	July 2013
RNA silencing in plants Baulcombe, David Nature, Vol. 431, Issue 7006 https://doi.org/10.1038/nature02874	journal	September 2004
Simultaneous knockdown of the expression of two genes using multiple shRNAs and subsequent knock-in of their expression Xu, Xue-Ming; Yoo, Min-Hyuk; Carlson, Bradley A. Nature Protocols, Vol. 4, Issue 9 https://doi.org/10.1038/nprot.2009.145	journal	August 2009
The Arabidopsis sex1 Mutant Is Defective in the R1 Protein, a General Regulator of Starch Degradation in Plants, and Not in the Chloroplast Hexose Transporter Yu, T. -S. THE PLANT CELL ONLINE, Vol. 13, Issue 8 https://doi.org/10.1105/tpc.13.8.1907	journal	August 2001
A high-throughput inducible RNAi vector for plants Wielopolska, Anna; Townley, Helen; Moore, Ian Plant Biotechnology Journal, Vol. 3, Issue 6 https://doi.org/10.1111/j.1467-7652.2005.00149.x	journal	November 2005
One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome Gibson, D. G.; Benders, G. A.; Axelrod, K. C. Proceedings of the National Academy of Sciences, Vol. 105, Issue 51 https://doi.org/10.1073/pnas.0811011106	journal	December 2008
GUN4, a Regulator of Chlorophyll Synthesis and Intracellular Signaling Larkin, R. M. Science, Vol. 299, Issue 5608 https://doi.org/10.1126/science.1079978	journal	February 2003
ChromDB: The Chromatin Database Gendler, Karla; Paulsen, Tara; Napoli, Carolyn Nucleic Acids Research, Vol. 36, Issue suppl_1 https://doi.org/10.1093/nar/gkm768	journal	October 2007
A Comprehensive Dataset of Genes with a Loss-of-Function Mutant Phenotype in Arabidopsis Lloyd, Johnny; Meinke, David Plant Physiology, Vol. 158, Issue 3 https://doi.org/10.1104/pp.111.192393	journal	January 2012
RNA Silencing in Plants: Yesterday, Today, and Tomorrow Eamens, Andrew; Wang, Ming-Bo; Smith, Neil A. Plant Physiology, Vol. 147, Issue 2 https://doi.org/10.1104/pp.108.117275	journal	June 2008
Analyzing real-time PCR data by the comparative CT method Schmittgen, Thomas D.; Livak, Kenneth J. Nature Protocols, Vol. 3, Issue 6 https://doi.org/10.1038/nprot.2008.73	journal	June 2008
A Modified MultiSite Gateway Cloning Strategy for Consolidation of Genes in Plants Vemanna, Ramu S.; Chandrashekar, Babitha K.; Hanumantha Rao, H. M. Molecular Biotechnology, Vol. 53, Issue 2 https://doi.org/10.1007/s12033-012-9499-6	journal	January 2012
Endogenous small interfering RNAs in animals Okamura, Katsutomo; Lai, Eric C. Nature Reviews Molecular Cell Biology, Vol. 9, Issue 9 https://doi.org/10.1038/nrm2479	journal	September 2008
Antiviral Immunity Directed by Small RNAs Ding, Shou-Wei; Voinnet, Olivier Cell, Vol. 130, Issue 3 https://doi.org/10.1016/j.cell.2007.07.039	journal	August 2007
Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans Fire, Andrew; Xu, SiQun; Montgomery, Mary K. Nature, Vol. 391, Issue 6669 https://doi.org/10.1038/35888	journal	February 1998
Roles of the different components of magnesium chelatase in abscisic acid signal transduction Du, Shu-Yuan; Zhang, Xiao-Feng; Lu, Zekuan Plant Molecular Biology, Vol. 80, Issue 4-5 https://doi.org/10.1007/s11103-012-9965-3	journal	September 2012
RNA interference Hannon, Gregory J. Nature, Vol. 418, Issue 6894 https://doi.org/10.1038/418244a	journal	July 2002
RNA interference-inducing hairpin RNAs in plants act through the viral defence pathway Fusaro, Adriana F.; Matthew, Louisa; Smith, Neil A. EMBO reports, Vol. 7, Issue 11 https://doi.org/10.1038/sj.embor.7400837	journal	October 2006
Yeast transformation: a model system for the study of recombination. Orr-Weaver, T. L.; Szostak, J. W.; Rothstein, R. J. Proceedings of the National Academy of Sciences, Vol. 78, Issue 10 https://doi.org/10.1073/pnas.78.10.6354	journal	October 1981
Complete Chemical Synthesis, Assembly, and Cloning of a Mycoplasma genitalium Genome Gibson, D. G.; Benders, G. A.; Andrews-Pfannkoch, C. Science, Vol. 319, Issue 5867 https://doi.org/10.1126/science.1151721	journal	February 2008
The evolution of RNAi as a defence against viruses and transposable elements Obbard, Darren J.; Gordon, Karl H. J.; Buck, Amy H. Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 364, Issue 1513 https://doi.org/10.1098/rstb.2008.0168	journal	October 2008
RNA-guided transcriptional regulation in planta via synthetic dCas9-based transcription factors Piatek, Agnieszka; Ali, Zahir; Baazim, Hatoon Plant Biotechnology Journal, Vol. 13, Issue 4 https://doi.org/10.1111/pbi.12284	journal	November 2014
RNA-Guided Genome Editing in Plants Using a CRISPR–Cas System Xie, Kabin; Yang, Yinong Molecular Plant, Vol. 6, Issue 6 https://doi.org/10.1093/mp/sst119	journal	November 2013
GUN4 Is Required for Posttranslational Control of Plant Tetrapyrrole Biosynthesis Peter, Enrico; Grimm, Bernhard Molecular Plant, Vol. 2, Issue 6 https://doi.org/10.1093/mp/ssp072	journal	November 2009
CATMA: a complete Arabidopsis GST database Crowe, M. L. Nucleic Acids Research, Vol. 31, Issue 1 https://doi.org/10.1093/nar/gkg071	journal	January 2003
RNA Silencing Pathways in Plants Herr, A. J.; Baulcombe, D. C. Cold Spring Harbor Symposia on Quantitative Biology, Vol. 69, Issue 0 https://doi.org/10.1101/sqb.2004.69.363	journal	January 2004
When more is better: multigene engineering in plants Naqvi, Shaista; Farré, Gemma; Sanahuja, Georgina Trends in Plant Science, Vol. 15, Issue 1 https://doi.org/10.1016/j.tplants.2009.09.010	journal	January 2010
Storage of competent cells for Agrobacterium transformation Hofgen, Rainer; Willmitzer, Lothar Nucleic Acids Research, Vol. 16, Issue 20 https://doi.org/10.1093/nar/16.20.9877	journal	January 1988
Synthesis of DNA fragments in yeast by one-step assembly of overlapping oligonucleotides Gibson, Daniel G. Nucleic Acids Research, Vol. 37, Issue 20 https://doi.org/10.1093/nar/gkp687	journal	September 2009
CRISPR-Cas systems for editing, regulating and targeting genomes Sander, Jeffry D.; Joung, J. Keith Nature Biotechnology, Vol. 32, Issue 4 https://doi.org/10.1038/nbt.2842	journal	March 2014
Artificial microRNA mediated gene silencing in plants: progress and perspectives Tiwari, Manish; Sharma, Deepika; Trivedi, Prabodh Kumar Plant Molecular Biology, Vol. 86, Issue 1-2 https://doi.org/10.1007/s11103-014-0224-7	journal	July 2014
A versatile approach to multiple gene RNA interference using microRNA-based short hairpin RNAs Zhu, Xiaocui; Santat, Leah A.; Chang, Mi BMC Molecular Biology, Vol. 8, Issue 1 https://doi.org/10.1186/1471-2199-8-98	journal	January 2007
Highly Specific Gene Silencing by Artificial MicroRNAs in Arabidopsis Schwab, Rebecca; Ossowski, Stephan; Riester, Markus The Plant Cell, Vol. 18, Issue 5 https://doi.org/10.1105/tpc.105.039834	journal	March 2006
Multi-miRNA hairpin method that improves gene knockdown efficiency and provides linked multi-gene knockdown Sun, Daqian; Melegari, Margherita; Sridhar, Sunandini BioTechniques, Vol. 41, Issue 1 https://doi.org/10.2144/000112203	journal	July 2006
DNA Cloning Using In Vitro Site-Specific Recombination Hartley, J. L. Genome Research, Vol. 10, Issue 11 https://doi.org/10.1101/gr.143000	journal	November 2000
Gene silencing in plants using artificial microRNAs and other small RNAs: Engineering small RNA-mediated gene silencing Ossowski, Stephan; Schwab, Rebecca; Weigel, Detlef The Plant Journal, Vol. 53, Issue 4 https://doi.org/10.1111/j.1365-313X.2007.03328.x	journal	February 2008
Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method Livak, Kenneth J.; Schmittgen, Thomas D. Methods, Vol. 25, Issue 4 https://doi.org/10.1006/meth.2001.1262	journal	December 2001
An effective gene-knockdown using multiple shRNA-expressing adenovirus vectors Motegi, Yukari; Katayama, Kazufumi; Sakurai, Fuminori Journal of Controlled Release, Vol. 153, Issue 2 https://doi.org/10.1016/j.jconrel.2011.04.009	journal	July 2011
A roadmap for research on crassulacean acid metabolism (CAM) to enhance sustainable food and bioenergy production in a hotter, drier world Yang, Xiaohan; Cushman, John C.; Borland, Anne M. New Phytologist, Vol. 207, Issue 3 https://doi.org/10.1111/nph.13393	journal	July 2015
Gene stacking in transgenic plants - the challenge for 21st century plant biotechnology: The challenge of transgene and trait stacking Halpin, Claire Plant Biotechnology Journal, Vol. 3, Issue 2 https://doi.org/10.1111/j.1467-7652.2004.00113.x	journal	February 2005
Construct design for efficient, effective and high-throughput gene silencing in plants: Construct design for gene silencing Wesley, S. Varsha; Helliwell, Christopher A.; Smith, Neil A. The Plant Journal, Vol. 27, Issue 6 https://doi.org/10.1046/j.1365-313X.2001.01105.x	journal	September 2001
Trial and Error: How the Unclonable Human Mitochondrial Genome was Cloned in Yeast Bigger, Brian W.; Liao, Ai-Yin; Sergijenko, Ana Pharmaceutical Research, Vol. 28, Issue 11 https://doi.org/10.1007/s11095-011-0527-1	journal	July 2011
The gun4 gene is essential for cyanobacterial porphyrin metabolism Wilde, Annegret; Mikolajczyk, Sandra; Alawady, Ali FEBS Letters, Vol. 571, Issue 1-3 https://doi.org/10.1016/j.febslet.2004.06.063	journal	July 2004
Small RNAs and regulation of transposons in plants Ito, Hidetaka Genes & Genetic Systems, Vol. 88, Issue 1 https://doi.org/10.1266/ggs.88.3	journal	January 2013
Automatic design of gene-specific sequence tags for genome-wide functional studies Thareau, V.; Dehais, P.; Serizet, C. Bioinformatics, Vol. 19, Issue 17 https://doi.org/10.1093/bioinformatics/btg286	journal	November 2003
Induced Deactivation of Genes Encoding Chlorophyll Biosynthesis Enzymes Disentangles Tetrapyrrole-Mediated Retrograde Signaling Schlicke, Hagen; Hartwig, Annabel Salinas; Firtzlaff, Vivien Molecular Plant, Vol. 7, Issue 7 https://doi.org/10.1093/mp/ssu034	journal	July 2014
RNAi: a defensive RNA-silencing against viruses and transposable elements Buchon, N.; Vaury, C. Heredity, Vol. 96, Issue 2 https://doi.org/10.1038/sj.hdy.6800789	journal	December 2005
A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures Murashige, Toshio; Skoog, Folke Physiologia Plantarum, Vol. 15, Issue 3, p. 473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x	journal	July 1962
An endogenous, systemic RNAi pathway in plants Dunoyer, Patrice; Brosnan, Christopher A.; Schott, Gregory The EMBO Journal, Vol. 29, Issue 10 https://doi.org/10.1038/emboj.2010.65	journal	April 2010
Arabidopsis Mutants Define a Central Role for the Xanthophyll Cycle in the Regulation of Photosynthetic Energy Conversion Niyogi, Krishna K.; Grossman, Arthur R.; Björkman, Olle The Plant Cell, Vol. 10, Issue 7 https://doi.org/10.1105/tpc.10.7.1121	journal	July 1998
MAX3/CCD7 Is a Carotenoid Cleavage Dioxygenase Required for the Synthesis of a Novel Plant Signaling Molecule Booker, Jonathan; Auldridge, Michele; Wills, Sarah Current Biology, Vol. 14, Issue 14 https://doi.org/10.1016/j.cub.2004.06.061	journal	July 2004
Specific cloning of human DNA as yeast artificial chromosomes by transformation-associated recombination. Larionov, V.; Kouprina, N.; Graves, J. Proceedings of the National Academy of Sciences, Vol. 93, Issue 1 https://doi.org/10.1073/pnas.93.1.491	journal	January 1996
Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana Clough, Steven J.; Bent, Andrew F. The Plant Journal, Vol. 16, Issue 6, p. 735-743 https://doi.org/10.1046/j.1365-313x.1998.00343.x	journal	December 1998
Molecular Mechanisms of RNA Interference Wilson, Ross C.; Doudna, Jennifer A. Annual Review of Biophysics, Vol. 42, Issue 1 https://doi.org/10.1146/annurev-biophys-083012-130404	journal	May 2013
Synthetic biology as it relates to CAM photosynthesis: challenges and opportunities DePaoli, Henrique C.; Borland, Anne M.; Tuskan, Gerald A. Journal of Experimental Botany, Vol. 65, Issue 13, p. 3381-3393 https://doi.org/10.1093/jxb/eru038	journal	February 2014
Efficient genome editing in plants using a CRISPR/Cas system Feng, Zhengyan; Zhang, Botao; Ding, Wona Cell Research, Vol. 23, Issue 10 https://doi.org/10.1038/cr.2013.114	journal	August 2013
Recombinase technology: applications and possibilities Wang, Yueju; Yau, Yuan-Yeu; Perkins-Balding, Donna Plant Cell Reports, Vol. 30, Issue 3 https://doi.org/10.1007/s00299-010-0938-1	journal	October 2010
RNA Interference: Biology, Mechanism, and Applications Agrawal, N.; Dasaradhi, P. V. N.; Mohmmed, A. Microbiology and Molecular Biology Reviews, Vol. 67, Issue 4 https://doi.org/10.1128/MMBR.67.4.657-685.2003	journal	December 2003
Endogenous siRNAs from naturally formed dsRNAs regulate transcripts in mouse oocytes Watanabe, Toshiaki; Totoki, Yasushi; Toyoda, Atsushi Nature, Vol. 453, Issue 7194 https://doi.org/10.1038/nature06908	journal	April 2008
Delivery of multiple transgenes to plant cells by an improved version of MultiRound Gateway technology Buntru, Matthias; Gärtner, Stefanie; Staib, Lena Transgenic Research, Vol. 22, Issue 1 https://doi.org/10.1007/s11248-012-9640-0	journal	September 2012
LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis Lee, Y. K.; Kim, G. -T.; Kim, I. -J. Development, Vol. 133, Issue 21 https://doi.org/10.1242/dev.02604	journal	October 2006
Engineering the Provitamin A (-Carotene) Biosynthetic Pathway into (Carotenoid-Free) Rice Endosperm Ye, Xudong; Al-Babili, Salim; Kloti, Andreas Science, Vol. 287, Issue 5451, p. 303-305 https://doi.org/10.1126/science.287.5451.303	journal	January 2000
Linker-Mediated Recombinational Subcloning of Large DNA Fragments Using Yeast Raymond, C. K. Genome Research, Vol. 12, Issue 1 https://doi.org/10.1101/gr.205201	journal	January 2002
The promoter of TL-DNA gene 5 controls the tissue-specific expression of chimaeric genes carried by a novel type of Agrobacterium binary vector Koncz, Csaba; Schell, Jeff MGG Molecular & General Genetics, Vol. 204, Issue 3 https://doi.org/10.1007/BF00331014	journal	September 1986
Versatile Gene-Specific Sequence Tags for Arabidopsis Functional Genomics: Transcript Profiling and Reverse Genetics Applications Hilson, P. Genome Research, Vol. 14, Issue 10b https://doi.org/10.1101/gr.2544504	journal	October 2004
Efficient downregulation of multiple mRNA targets with a single shRNA-expressing lentiviral vector Chumakov, Stepan P.; Kravchenko, Julia E.; Prassolov, Vladimir S. Plasmid, Vol. 63, Issue 3 https://doi.org/10.1016/j.plasmid.2009.12.003	journal	May 2010
QuantPrime - a flexible tool for reliable high-throughput primer design for quantitative PCR Arvidsson, Samuel; Kwasniewski, Miroslaw; Riano-Pachon, Diego M. BMC Bioinformatics, Vol. 9, Issue 1 https://doi.org/10.1186/1471-2105-9-465	journal	January 2008
RNA silencing platforms in plants Watson, John M.; Fusaro, Adriana F.; Wang, MingBo FEBS Letters, Vol. 579, Issue 26 https://doi.org/10.1016/j.febslet.2005.08.014	journal	August 2005
Reverse genetics techniques: engineering loss and gain of gene function in plants Gilchrist, E.; Haughn, G. Briefings in Functional Genomics, Vol. 9, Issue 2 https://doi.org/10.1093/bfgp/elp059	journal	January 2010
pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation Hellens, Roger P.; Edwards, E. Anne; Leyland, Nicola R. Plant Molecular Biology, Vol. 42, Issue 6, p. 819-832 https://doi.org/10.1023/A:1006496308160	journal	January 2000
The Development of C ₄ Rice: Current Progress and Future Challenges von Caemmerer, Susanne; Quick, W. Paul; Furbank, Robert T. Science, Vol. 336, Issue 6089 https://doi.org/10.1126/science.1220177	journal	June 2012
Arabidopsis Mutants Define a Central Role for the Xanthophyll Cycle in the Regulation of Photosynthetic Energy Conversion Niyogi, Krishna K.; Grossman, Arthur R.; Bjorkman, Olle The Plant Cell, Vol. 10, Issue 7 https://doi.org/10.2307/3870716	journal	July 1998

Cited By (4)

Systems models, phenomics and genomics: three pillars for developing high-yielding photosynthetically efficient crops Chang, Tian-Gen; Chang, Shuoqi; Song, Qing-Feng in silico Plants, Vol. 1, Issue 1 https://doi.org/10.1093/insilicoplants/diy003	journal	January 2019
REM34 and REM35 Control Female and Male Gametophyte Development in Arabidopsis thaliana Caselli, Francesca; Beretta, Veronica Maria; Mantegazza, Otho Frontiers in Plant Science, Vol. 10 https://doi.org/10.3389/fpls.2019.01351	journal	October 2019
A copper-controlled RNA interference system for reversible silencing of target genes in Trichoderma reesei Wang, Lei; Zheng, Fanglin; Zhang, Weixin Biotechnology for Biofuels, Vol. 11, Issue 1 https://doi.org/10.1186/s13068-018-1038-7	journal	February 2018
A novel hairpin library-based approach to identify NBS–LRR genes required for effector-triggered hypersensitive response in Nicotiana benthamiana Brendolise, Cyril; Montefiori, Mirco; Dinis, Romain Plant Methods, Vol. 13, Issue 1 https://doi.org/10.1186/s13007-017-0181-7	journal	April 2017

Linked Research (6)

Similar Records

Interference RNA (RNAi)-based silencing of endogenous thrombopoietin receptor (Mpl) in Dami cells resulted in decreased hNUDC-mediated megakaryocyte proliferation and differentiation

Journal Article · Thu Dec 10 00:00:00 EST 2009 · Experimental Cell Research · OSTI ID:1618904

Pang, Shi-Feng; Li, Xiao-Kun; Zhang, Qiang; +2 more

The RNAi machinery controls distinct responses to environmental signals in the basal fungus Mucor circinelloides

Journal Article · Wed Mar 25 00:00:00 EDT 2015 · BMC Genomics · OSTI ID:1618904

Nicolas, Francisco E.; Vila, Ana; Moxon, Simon; +4 more

Evaluation of synthetic promoters in Physcomitrella patens

Journal Article · Fri Jun 15 00:00:00 EDT 2018 · Biochemical and Biophysical Research Communications · OSTI ID:1618904

Peramuna, Anantha; Bae, Hansol; Mosspiration Biotech IVS, Hørsholm; +7 more

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
58 GEOSCIENCES

Simultaneous knockdown of six non-family genes using a single synthetic RNAi fragment in Arabidopsis thaliana [Supplemental Data] Czarnecki, Olaf; Bryan, Anthony; Jawdy, Sara https://doi.org/10.6084/m9.figshare.c.3645857 The current record is supplemented by this collection	collection	February 2016
Simultaneous knockdown of six non-family genes using a single synthetic RNAi fragment in Arabidopsis thaliana [Supplemental Data] Czarnecki, Olaf; Bryan, Anthony; Jawdy, Sara https://doi.org/10.6084/m9.figshare.c.3645857.v1 The current record is supplemented by this collection	collection	February 2016
MOESM1 of Simultaneous knockdown of six non-family genes using a single synthetic RNAi fragment in Arabidopsis thaliana Czarnecki, Olaf; Bryan, Anthony; Jawdy, Sara https://doi.org/10.6084/m9.figshare.c.3645857_d1 The current record is supplemented by this dataset	dataset	February 2016
MOESM2 of Simultaneous knockdown of six non-family genes using a single synthetic RNAi fragment in Arabidopsis thaliana Czarnecki, Olaf; Bryan, Anthony; Jawdy, Sara https://doi.org/10.6084/m9.figshare.c.3645857_d2 The current record is supplemented by this dataset	dataset	February 2016
MOESM1 of Simultaneous knockdown of six non-family genes using a single synthetic RNAi fragment in Arabidopsis thaliana Czarnecki, Olaf; Bryan, Anthony; Jawdy, Sara https://doi.org/10.6084/m9.figshare.c.3645857_d1.v1 The current record is supplemented by this dataset	dataset	February 2016
MOESM2 of Simultaneous knockdown of six non-family genes using a single synthetic RNAi fragment in Arabidopsis thaliana Czarnecki, Olaf; Bryan, Anthony; Jawdy, Sara https://doi.org/10.6084/m9.figshare.c.3645857_d2.v1 The current record is supplemented by this dataset	dataset	February 2016